Bacterial Genetics and Biotechnology

The paper uses Deep Mutational Scanning and Growth-based Quantitative Sequencing to analyze CymR variants and identify amino acids essential for repressor function and mutations that improve ligand sensitivity.

This study identifies the role of conserved lysine residues in ParB's C-terminal domain in DNA sliding and in vivo function.

This study shows that positioning RNAP-encoding genes near the replication origin in fast-growing bacteria is essential for optimal growth and gene dosage effects.

This paper uses molecular dynamics simulations to study how E. coli TatA oligomers interact with Tat translocon substrates, showing structural changes and lipid interactions during protein transport.

The paper identifies novel origins of transfer (oriTs) in Genomic Islands, revealing their role as hitchhikers on conjugative elements and their contribution to bacterial genetic adaptation.

This paper shows that DNA replication initiation in E. coli sets a fixed mass increment for cell division, resolving conflicting models of division timing.

The paper identifies the functionally asymmetric TraI homodimer structure and elucidates how strand nicking and helicase loading are coordinated during bacterial conjugation.

The paper shows that basal ppGpp levels in E. coli are actively regulated by SpoT and are essential for metabolic homeostasis and acid resistance.

This paper shows that loss of DNA mismatch repair genes in E. coli and Salmonella increases antibiotic resistance independently of secondary mutations through increased homoeologous recombination and cell lysis.

This study compares transcriptomic responses of Chlamydia trachomatis to different stressors, revealing distinct reprogramming and shared features across conditions.

This study measures the biophysical properties of the bacterial nucleoid and shows they are dynamic, heterogeneous, and responsive to cellular processes.

The paper examines how bacterial cell volume changes over the growth curve in response to nutrient conditions using microscopy and mathematical modeling, finding non-monotonic dynamics in biomass-to-cell-number ratios.

The paper shows that pilin cyclisation and pilus formation are robust to amino acid substitutions, but electrostatic changes impair conjugation efficiency.

The paper identifies a conserved transporter-repressor module in streptococci that directly couples bacteriocin detection to transcriptional activation, revealing a minimalist and rapid mechanism for antimicrobial peptide resistance.

This study investigates the transcription initiation mechanism in spirochetes, revealing unique structural and functional features of their RNA polymerase.

The paper shows how transcriptional heterogeneity and redundancy in stress responses shape antibiotic persistence in Klebsiella pneumoniae using single-cell RNA sequencing and functional assays.

This study defines the substrate binding and activation mechanism of FtsW using simulations, mutagenesis, and assays, revealing conserved residues and structural constraints involved in lipid II binding and catalysis.

This study identifies an aromatic patch in Mtb Wbl proteins that mediates interaction with the primary sigma 70 factor, revealing a conserved binding mechanism and evolutionary relationship with actinobacterial phages.

The paper introduces NBx CyClone, an engineered Vibrio natriegens strain, as a faster and more efficient molecular cloning host compared to traditional E. coli strains.

The paper identifies and characterizes a fourth type VI secretion system (H4-T6SS) in Pseudomonas aeruginosa, showing it is repressor-gated and contributes to inter- and intraspecific antagonism.

This paper introduces an automated method to estimate the DNA replication cycle duration in E. coli using flow cytometry data without requiring pharmacological treatments.

This study shows that chlamydial histones HctA and HctB are essential for transgenerational developmental fitness, as their deficiency during EB maturation impairs subsequent infection cycles.

This study investigates how H-NS and StpA regulate gene expression in E. coli under acid and osmotic stress by analyzing transcription, DNA binding, and chromatin structure at specific operons.

The paper studies bacterial iron acquisition under low carbon conditions and finds that amino acids facilitate iron uptake by E. coli.

This paper identifies a metal-responsive UMPylation mechanism in Salmonella that enables intracellular biphasic growth and antibiotic tolerance by modulating protein aggregation and translational arrest.

The paper shows conjugative plasmids eliminate non-permissive bacterial recipients through lethal zygosis, enabling their own spread and improving gene editing efficiency with shielded transposon vectors.

This paper investigates the role of TlpD's C-terminus in its polar localization and chemotaxis function in H. pylori, showing that loss of the C-terminus disrupts localization and chemotactic ability.

This study examines the role of spoVF operon subunits A and B in regulating cell morphology, biofilm formation, and biocontrol ability in Bacillus subtilis, finding that their knockout reduces DPA biosynthesis and biocontrol efficacy.

The paper identifies DedD as an interacting partner of YhdP and shows that AsmA-superfamily proteins are enriched at Escherichia coli cell poles, suggesting spatial restriction of phospholipid transport.