Protein Kinase Regulation and GTPase Signaling

The paper identifies Smog as a GPCR that transduces Fog signals to regulate Rho kinase and myosin activation during Drosophila salivary gland invagination, revealing its role in apical constriction and epithelial integrity.

The paper uses optogenetic actuator and ERK biosensor circuits to study MAPK network dynamics and identifies RSK2-mediated feedback as critical for ERK regulation and drug response.

The paper develops a kinase translocation reporter to monitor ERK activity in real-time in Drosophila and identifies novel dynamics in ERK signaling during development.

The paper classifies over 700 RAS structures into conformations based on SW1 and SW2 loops, identifies mutation impacts, and creates a database for analysis.

The paper analyzes LZTR1 loss-of-function mutants in fruit flies and mice, showing dependency on RIT1 orthologs and haplosufficiency in mice.

This paper designs a CDC42 effector interaction inhibitor (ARN22089) that shows anti-cancer activity by blocking tumor invasion and angiogenesis.

The paper shows that PKA-R translocation to the plasma membrane can both enhance and inhibit kinase activity depending on concentration, affecting cell migration directionality.

This study compares the structure, function, and high-order assembly of neuronal CaMKII and β-isoforms using single-particle EM and biochemical methods, revealing differences in their extended states and clustering behavior.

The paper shows KRAS mutations disrupt its interaction with LZTR1, affecting protein stability via the CUL3 ubiquitin ligase complex, contributing to oncogenesis.

The paper investigates how the GTPase Ran/Gsp1 achieves functional multi-specificity through biophysical and genetic perturbations, revealing that its effects are linked to kinetic parameters of the switch cycle rather than direct interaction sites.

The paper proposes an atomistic structural model of a Ras-Raf signalosome based on molecular dynamics simulations, mutagenesis, electron microscopy, and cell-based experiments.

The paper uses CRISPR to tag ERK-1 in C. elegans and shows nuclear translocation as a marker of activation events during development.

This paper identifies two RAS-binding Affimer proteins that inhibit RAS function and reveal a novel druggable SII/3 pocket.

The paper shows that phosphorylation of the yeast Gγ subunit Ste18 at multiple sites modulates its structure and affects MAPK activation.

This study identifies zDHHC3 as a critical regulator of cardiac hypertrophy by enhancing Rac1 S-palmitoylation and RhoGTPase signaling in cardiomyocytes.

The paper identifies the roles of RalGEF>Ral, Rap1, and Ras signaling in C. elegans nervous system development, showing Ral's permissive role and Ras's instructive role in cell migration.

The paper investigates how serotonin signaling through Gq, PLCβ, and Trio RhoGEF promotes egg-laying behavior in C. elegans by modulating DAG levels and distinct effector pathways.

The paper identifies a GTPase circuit that enables secretion-coupled cellular autonomy in cancer cells through feedback loops and dose response alignment of growth factor sensing and secretion.

The paper develops optogenetic tools to control Rac1 and Rap1 activity in living cells, showing increased membrane localization and effects on cell morphology.

The paper identifies RpS21 as a signal strength-dependent regulator of the Ras/MAPK pathway using codon usage manipulation in Drosophila.

This study identifies and characterizes a novel 44-mer peptide from DISC1 that inhibits GSK3β in a non-competitive manner, offering a new approach for developing selective GSK3β inhibitors.

The paper identifies genes with biphasic expression in response to increasing cAMP levels and shows PKA and ERK1/2 exhibit opposite biphasic responses, revealing complex signaling integration.

The paper quantifies ERK activity in RAS-mutant cells and finds that mutant KRAS isoforms produce similar ERK output ranges despite differing biochemical activity, due to pathway-level rescaling mechanisms.

The paper shows that the regulatory segment of CaMKII can break the oligomeric hub into smaller assemblies through binding and structural destabilization.

The paper shows how periodic GTPase waves coordinate leading and trailing edge dynamics during cell migration through RhoA-Rac1 interactions.

The paper investigates how Cdc42p adaptors regulate the fMAPK pathway in yeast, showing distinct activation kinetics and sequential interactions between Bem4p and Bem1p.

The paper shows that Rho-kinase membrane residence time is regulated by phospho-modulation at tissue boundaries, affecting planar polarization during morphogenesis.

The paper develops a rule-based computational model of CaMKII holoenzyme to explore how Ca2+/CaM binding stabilizes autophosphorylation and regulates synaptic plasticity.

The paper introduces an ultrasensitive fiveplex assay to measure multiple kinase activities simultaneously, demonstrating its application in studying viral infection.