Lipid Membrane Structure and Behavior

Size Selection of Giant Unilamellar Vesicles (GUVs) via Modified cDICE Method

This study optimizes the modified cDICE method to control GUV size distribution by adjusting rotation time and angular frequency.

Shape analysis of biomimetic and plasma membrane vesicles

The paper compares shape deformations of biomimetic and plasma membrane vesicles under different lipid and solution conditions using 3D visualization.

Stability vs flexibility: reshaping archaeal membranes in silico

The paper uses a computational model to study how archaeal membrane lipids balance stability and flexibility, finding that adding bilayer lipids to bolalipid membranes achieves both.

High Frequency MHz-Order Vibration Enables Cell Membrane Remodelling and Lipid Microdomain Manipulation

The paper demonstrates that MHz-order vibrations induce cell membrane remodelling by altering lipid microdomains, activating Piezo1 channels, and promoting tau protein aggregation in neuroblastoma cells.

A comparison of lipid diffusive dynamics in monolayers and bilayers in the context of interleaflet coupling

This study compares lipid diffusive dynamics in monolayers and bilayers to understand interleaflet coupling mechanisms.

Chemically defined lipid diets reveal the versatility of lipidome remodeling in genomically minimal cells

The paper uses chemically defined lipid diets to study how lipidome complexity affects membrane function and cell phenotypes in minimal cells.

High-speed imaging of giant unilamellar vesicle formation in cDICE

The paper develops a high-speed microscopy setup to study GUV formation in cDICE, revealing size-selective crossing at the oil-water interface and the effect of proteins on GUV formation.

Simulation-based survey of TMEM16 family reveals that robust lipid scrambling requires an open groove

This study uses simulations to show that TMEM16 proteins primarily scramble lipids in an open groove when Ca2+ is bound, but also do so at the dimer interface when Ca2+ is absent.

Chaotropic Agent-assisted Supported Lipid Bilayer Formation

This paper introduces a new method for forming supported lipid bilayers on diverse substrates using a chaotropic agent exchange process, demonstrating its effectiveness and utility for sensing applications.

Challenges in the Accurate Modelling of Lipid Dynamics in Monolayers and Bilayers

This study investigates lipid diffusion in bilayers and monolayers, finding that water model properties significantly affect dynamics and suggesting the need for lipid model modifications.

Contrasting Effects of Cytoskeleton Disruption on Plasma Membrane Receptor Dynamics: Insights from Single-Molecule Analyses

This study uses single-molecule tracking to examine how disrupting the cytoskeleton affects plasma membrane receptor dynamics in plant cells, revealing distinct effects of actin and microtubule disruption.

Lipid vesicle formation by encapsulation of SMALPs in surfactant-stabilised droplets.

This paper introduces a method to create GUVs using SMALPs and surfactant-stabilised droplets, resulting in porous vesicles suitable for studying membrane proteins.

Lipid loss and compositional change during preparation of liposomes by common biophysical methods

This paper quantifies lipid loss and compositional changes during liposome preparation using HPLC-ELSD and identifies best practices to maximize lipid recovery and minimize composition bias.

Tensing Flipper: Photosensitized manipulation of membrane tension, lipid phase separation and raft protein sorting in biological membranes

This paper discovers that Flipper, a membrane tension probe, can photosensitize singlet oxygen to alter membrane tension and lipid phase separation, enabling optical control of lipid raft formation.

Molecular Dynamics of Phosphatidylcholine Model Membranes of Splenic Lymphoma Cells

This study uses molecular dynamics simulations to compare phosphatidylcholine membrane properties between pure and cancer model membranes, finding differences in surface area, chain order, and bilayer thickness.

Solvatochromic reporter to image plasma membrane order leaflet by leaflet reveals ahighly asymmetric bilayer locally modulated by transbilayer interactions

The paper introduces a solvatochromic probe, C3L, to measure membrane order at the plasma membrane leaflet level, revealing asymmetry and local modulation by transbilayer interactions.

Faster but not sweeter: A model of E. coli Re-level lipopolysaccharide for Martini 3 and a Martini 2 version with accelerated kinetics

The paper introduces a faster-diffusing LPS model for Martini 2 and completes the Martini 3 LPS toolkit, enabling better simulation of membrane interactions and dynamics.

Plasma membrane folding enables constant surface area-to-volume ratio in growing mammalian cells

The paper shows that mammalian cells maintain a constant surface area-to-volume ratio as they grow by increasing plasma membrane folding.

Intrinsically disordered membrane anchors of Rheb, RhoA and DiRas3 small GTPases: Molecular dynamics, membrane organization, and interactions

This study uses molecular dynamics simulations to analyze how intrinsically disordered regions of small GTPases interact with membranes, showing that lipid sequence complementarity and electrostatic interactions influence conformational dynamics and lipid sorting.

Ligandability at the membrane interface of GPx4 revealed through a reverse micelle fragment screening platform

This study develops a reverse micelle fragment screening method to identify non-covalent ligands for membrane-bound GPx4, revealing ligandability at the protein-membrane interface.

Disruption of liquid/liquid phase separation in asymmetric GUVs prepared by hemifusion

The paper uses calcium-induced hemifusion to create asymmetric GUVs and studies phase behavior changes, finding variability in outer leaflet exchange and correlation with asymmetry.

Ratiometric fluorescence nanoscopy and lifetime imaging of novel Nile Red analogs for analysis of membrane packing in living cells

This study evaluates modified Nile Red dyes for super-resolution imaging of membrane packing in cells, finding differences in membrane structure between healthy and NPC1 disease fibroblasts.

Acidic Sphingomyelinase Interactions with Lysosomal Membranes and Cation Amphiphilic Drugs: a Molecular Dynamics Investigation

This study uses molecular dynamics simulations to investigate how acidic sphingomyelinase interacts with lysosomal membranes and how cation amphiphilic drugs like ebastine affect these interactions.

Manipulation of encapsulated artificial phospholipid membranes using sub-micellar lysolipid concentrations.

This paper demonstrates the manipulation of encapsulated artificial membranes using lysolipid concentrations to control their structure and properties.

Crosstalk between plasma membrane and Staphylococcus α-hemolysin during oligomerization

This study investigates the structural and functional dynamics of Staphylococcus aureus α-hemolysin during membrane interaction, revealing its role in cell necrosis and pore formation.

Membrane specificity of the human cholesterol transfer protein STARD4

This study investigates how human STARD4 binds to membranes and transfers cholesterol, showing lipid composition and cargo influence its activity.

Hydrophobic mismatch drives self-organization of designer proteins into synthetic membranes

This study investigates how hydrophobic mismatch affects membrane protein organization using protein design, simulations, and cell-free systems, showing deformation of membranes and controlled protein assembly.

DIB-BOT: An open-source hardware approach for high throughput droplet interface bilayer deposition

DIB-BOT is an open-source hardware system for rapid, reproducible DIB fabrication, reducing errors and increasing yield compared to manual methods.

The Mycobacterium lipid transporter MmpL3 is dimeric in detergent solution, SMALPs and reconstituted nanodiscs

This paper shows MmpL3 from M. smegmatis forms dimers in various membrane mimetic systems, contradicting previous structural studies suggesting monomerism.

Ultrahigh yields of giant vesicles obtained through mesophase evolution and breakup

This paper discovers a new pathway for forming giant unilamellar vesicles (GUVs) with ultrahigh yields (>50%) using a lipid-dense mesophase and fluid shear.