Heat shock proteins research

The paper identifies LarA as a regulator of Lon protease activation during proteotoxic stress, showing its role in modulating proteolysis through a specific C-terminal sequence.

This study identifies Hsc70 and Hsp70 as cellular proteins interacting with MVC viral proteins and shows their distinct roles in MVC infection, suggesting Hsp70 as a potential therapeutic target.

This study identifies the Plasmodium prefoldin complex as essential for proteotoxic stress response and demonstrates that biperiden inhibits its function, offering a new drug target for malaria.

This study identifies Asc1p and Hel2p as RQC factors that regulate HSP70 mRNA translation and degradation during heat stress and recovery in yeast.

The paper defines and annotates 959 genes comprising the human proteostasis network, focusing on translation, protein folding, and organelle-specific systems.

The paper identifies transcellular chaperone signaling (TCS) as a gut-to-muscle stress response in C. elegans that bypasses HSF-1-mediated heat stress resistance.

This study reveals the structural and functional mechanism of CLPB, a mitochondrial AAA+ disaggregase, showing its unique assembly and substrate translocation properties.

This study uses a fluorescent reporter substrate to elucidate the stepwise mechanism of Hsp70 unfoldase action in converting aggregates into native proteins.

The paper determines the cryo-EM structure of yeast Lon protease (PIM1) and shows it forms a hexamer similar to bacterial and human Lon proteases, with unique structural features and a dispensable yeast-specific insertion.

This study shows HSF1 and HSF2 regulate gene and enhancer transcription differently under oxidative stress and heat shock by controlling RNA polymerase II pause-release.

This paper uses cross-linking mass spectrometry to characterize Hsp70 interactions in budding yeast, revealing self-association, multi-point client interactions, and novel PTMs critical for client function.

The paper identifies sequence features of aggregation-prone regions in yeast proteins using machine learning on proteolysis-mass spectrometry data.

The paper identifies a germinal cell-specific NAC paralog in Drosophila with expanded intrinsically disordered regions and shows it can suppress the lethal effect of losing the ubiquitous NAC-β subunit.

The paper measures HSP90 and HSP60 expression in Daphnia magna under thermal stress and mutation accumulation, finding increased expression and synergistic effects.

The paper identifies a de novo FOXR1 variant causing microcephaly and brain atrophy by impairing stress response pathways.

The paper uses CRISPRi/a to screen gene functions in yeast, identifying core signaling pathways and novel interactions involved in heat adaptation and stress response.

This paper identifies AscA (YecA) as a novel molecular chaperone that facilitates Sec-dependent protein translocation in E. coli by interacting with SecB and ribosomes.

The paper uses in-silico methods to analyze the molecular interactions between IbpA, IbpB, and a mutant Lon protease with S679A mutation in E. coli under physiological temperature.

The paper identifies mutations in the yeast Hsf1 oligomerization domain that alter transcriptional programs and fitness under heat stress.

The paper clones and characterizes a novel Heat Shock Factor (Hsf2) gene from wheat using in silico methods and evaluates its 3D structure.

This study shows that misfolded proteins in the heart can disrupt brain function and worsen stroke damage through prion-like mechanisms.

The paper identifies hsp-16.2 chaperone biomarkers as indicators of proteome dosage states in C. elegans, revealing cell-to-cell variation linked to gene expression differences.

The paper determines the cryo-EM structure of Y. pestis Lon protease during substrate processing, revealing a distinct hand-over-hand translocation mechanism unique to the HCLR clade.

The paper investigates the role of bacterial Hsp90 (HtpG) in collaboration with Hsp70 (DnaK) and shows that HtpG mediates degradation of aggregation-prone substrates via HslUV proteolysis.

This study shows that heat shock stress activates transposon expression by disrupting piRNA biogenesis through Hsp70's interaction with the Hsc70-Hsp90 complex in Drosophila.

This study shows that HSP90 reduces the penetrance of newly introduced mutations in plants, leading to fewer embryonic lethality and phenotypic abnormalities compared to HSP90-reduced plants.

This study identifies that loss of rsks-1/S6-Kinase rescues developmental arrest in C. elegans hsf-1 mutants, revealing an unexplained regulatory link between heat-shock transcription factors and the TOR pathway during development.

This study investigates the holdase function of Escherichia coli DnaK chaperone using single-molecule assays, showing it stabilizes unfolded proteins and switches between holding and releasing states upon ATP binding.