Heat shock proteins research

The cysteine-rich domain of SEP15, a selenoprotein co-chaperone of the ER chaperone, UDP-glucose:glycoprotein glucosyltransferase, adopts a novel fold

The paper determines the structure of the cysteine-rich domain of SEP15 using AlphaFold and NMR, revealing a novel helical fold stabilized by disulfide bonds.

The mitochondrial chaperone HSPD1 folds MTHFD2 independently of its co-chaperone HSPE1

This study identifies HSPD1 as a chaperone for MTHFD2, independent of HSPE1, and shows distinct functional roles for HSPD1 and HSPE1 in mitochondrial biology.

Drosophila Heat Shock Factor (HSF) Regulates Developmental Growth by Maintaining the Basal Expression of HSP83/HSP90

This study shows that HSF maintains basal HSP83/HSP90 expression to support developmental growth in Drosophila, with loss of HSF causing developmental defects and reduced chaperone levels.

Small-molecule activators of the Staphylococcus aureus ClpC/ClpP AAA+ protease

The paper identifies small-molecule activators of the Staphylococcus aureus ClpC/ClpP protease through high-throughput screening and characterizes their binding sites.

Function within Disorder: Small heat shock proteins use different functional regions to chaperone tau aggregation

This study shows HSPB1 and HSPB5 use distinct N-terminal regions to delay tau aggregation, with HSPB1 under normal conditions and HSPB5 under stress.

Coordinated regulation of the metaboproteome by Hsp90 chaperones controls metabolic plasticity

This study shows that Hsp90 chaperones regulate the metaboproteome to control metabolic plasticity by affecting mitochondrial function and metabolic flexibility.

The chloroplast CLPD chaperone: consequences of under- and overexpression, interaction with the CLP protease core, and candidate substrates

This study investigates the role of CLPD in chloroplast proteostasis by analyzing its expression, developmental effects, and interactions with the CLP protease core and candidate substrates in Arabidopsis.

TRiC/CCT Chaperonin Governs RNA Polymerase II Activity in the Nucleus to Support RNA Homeostasis

This study shows TRiC/CCT regulates RNA polymerase II activity in the nucleus, affecting RNA homeostasis by modulating nascent RNA production.

Loss of mitochondrial co-chaperone GRPEL2 protects mice from age- and diet-induced obesity

This study shows that loss of GRPEL2 in mice protects against age- and diet-induced obesity and improves metabolic health without affecting mitochondrial protein import.

Azadiradione regulates Heat Shock Factor 1 function by interacting with its DNA-binding domain independent of the oligomerization domain

This study shows azadiradione activates HSF1 by interacting with its DNA-binding domain, promoting oligomerization and HSE binding, independent of the oligomerization domain.

Direct single-molecule visualization of Hsp90-mediated relief of a Hsp70-folding block

This study uses single-molecule techniques to show how Hsp90 helps Hsp70 release folding intermediates and remodel client proteins to enable proper refolding.

Mapping the chaperonin TRiC/CCT interactome in mouse photoreceptors reveals functional significance for energy metabolism

The paper maps the TRiC/CCT interactome in mouse photoreceptors and identifies its role in energy metabolism and neurodegeneration.

Dynamic global acetylation remodeling during the yeast heat shock response

The paper identifies dynamic acetylation changes in yeast during heat shock, showing acetylation regulates protein activity to enhance the heat shock response.

TRiC-assisted folding of class I HDAC family proteins regulated by distinct co-chaperone and cofactor networks

This study elucidates the TRiC-assisted folding of class I HDACs, revealing distinct co-chaperone and cofactor networks for HDAC1 and HDAC3, while HDAC8 folds independently.

Structural basis of HSP90C, a highly active chloroplastic HSP90 chaperone from A. thaliana

This study identifies non-canonical mechanisms of HSP90C, a chloroplastic HSP90 chaperone, through structural and biophysical analyses, revealing features contributing to its high ATPase activity.

A non-canonical role for UPRER during heat stress in C. elegans.

This study shows heat stress activates UPRER through XBP-1 and HSF-1 in C. elegans, with cross-talk to cytosolic HSR and inter-tissue stress communication enhancing thermotolerance.

Conformational dynamics of the membrane-anchored foldase LipH from Pseudomonas aeruginosa governs recognition and release of its client lipase

This study investigates the conformational dynamics of the membrane-anchored foldase LipH and shows how it recognizes, folds, and releases its client lipase LipA.

Dimerization of natively folded molecules drives misfolding and aggregation in Cys-depleted variants of cataract-associated human lens γD-crystallin

This study investigates how Cys depletion in γD-crystallin leads to misfolding and aggregation through dimerization, revealing a novel pathway for age-related cataract formation.

Activation Mechanism and Structural Assembly of the Mycobacterium tuberculosis ClpP1P2 Protease and Its Associated ATPases.

This study identifies trehalose as an activator of ClpC1P1P2 and ClpXP1P2 protease complexes in Mycobacterium tuberculosis, revealing a new mechanism for in vivo activation without the need for peptide activators.

Deletion of Murine Endothelial Bag3 Alters the Cellular Proteome Among Organs of Origin

This study shows that deleting BAG3 in endothelial cells uniquely alters the proteome of specific organs, with the most significant changes in skeletal muscle.

Cryo-EM structure of the FtsH periplasmic domain reveals functional dynamics

The paper reports cryo-EM structures of the Escherichia coli FtsH periplasmic domain, revealing conformational flexibility and its role in substrate translocation.

Chaperonin TRiC bridges radial spokes for folding proteins locally translated in mammalian sperm flagella

The paper identifies TRiC/CCT as a chaperonin in mammalian sperm flagella that folds locally translated proteins, supporting flagellar function and fertility.

Structural Plasticity of the Membrane-Bound Protein Degradation Assembly Supports Bacterial Adaptation to Stress

The paper investigates the structural plasticity of the HflK/C complex in bacteria, showing that both open and closed conformations are critical for adaptation to aminoglycoside stress.

Disulfide Crosslinking Induces Rapid Degradation of Arc/Arg3.1 via Hsp70-Mediated Ubiquitin Ligase Pathway

This study identifies a novel degradation pathway for Arc/Arg3.1, showing that disulfide crosslinking promotes rapid degradation via Hsp70-mediated ubiquitination and affects heat shock response.

Small heat shock proteins HspB1 and HspB5 differentially alter the condensation and aggregation of the TDP-43 low complexity domain

This paper shows that HspB1 and HspB5 differentially regulate TDP-43LCD condensation and aggregation, with HspB1 increasing dynamic exchange and HspB5 delaying pathological transition.

Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress

The paper uses cryo-electron tomography to study structural changes in mitochondrial protein biogenesis under proteostatic stress, revealing remodeling of cristae and mHsp60 conformation.

Differential inclusion formation of an aggregation-prone protein reveals differences in the proteostasis capacity of neuronal cell lines

The paper compares proteostasis capacity in two neuronal cell lines by analyzing inclusion formation of an aggregation-prone protein, revealing differences in proteostasis machinery engagement.

Domain-specific folding of the tandem β-propeller protein Coronin 7 (Coro7) by CCT/TRiC

This study identifies Coronin 7 as a CCT/TRiC substrate and shows that CCT/TRiC interacts specifically with the first β-propeller domain of Coro7.

HSP-1-Specific Nanobodies Alter Chaperone Function in vitro and in vivo

This study develops two nanobodies that specifically target HSP-1, reducing its ATPase activity and protein folding capacity both in vitro and in vivo.

Characterization of heat shock protein expression and its application to temporal gene expression in the nematode Pristionchus pacificus

The paper identifies a heat shock promoter in Pristionchus pacificus for temporal gene expression by analyzing heat-induced gene expression through RNA-seq and reporter transgenesis.