Discover, rate, and verify biology preprints.

Ratio-Free Detection and Partial Field Illumination Improve Time-Domain Dynamic Full-Field Optical Coherence Tomography Sensitivity for Retinal Organoid Imaging

QRS Detection by Combinatorial Optimization With MLP Assisted Peak Scoring

This paper introduces a QRS detector using MLP-assisted peak scoring and combinatorial optimization, achieving high sensitivity and PPV in noisy signals.

Oxygen-Releasing Hydrogel Patches Restore pH Balance and Support Cell Survival in Acidic Oral Wound Models

This paper develops a hydrogel patch that releases oxygen and buffers acidity in oral wound models, improving cell viability and pH balance.

Preventing trogocytosis by cathepsin B inhibition augments CAR T cell function

This study shows that inhibiting cathepsin B prevents CAR T cell fratricide and exhaustion by blocking trogocytosis, improving CAR T cell persistence and anti-tumor activity.

Ultra-long stable biomimetic nanoparticle Click-ed-to-cancer membrane for anti-cancer treatment

The paper develops a stable biomimetic nanoparticle using click-chemistry to coat cancer cell membranes for anti-cancer treatment.

Transplantation of active mitochondria condensed in liquid-liquid phase-separated hydrogels ameliorates myocardial ischemia-reperfusion injury

This study develops a liquid-liquid phase-separated hydrogel to deliver mitochondria for treating myocardial ischemia-reperfusion injury, showing improved mitochondrial function and cardiac recovery.

Modular fluidic automation platform with integrated thermal control for multi-step molecular imaging workflows

This paper introduces two open-source robots for automated multi-step mRNA imaging workflows, enabling precise buffer exchanges, temperature control, and scalable execution of multiplex-FISH experiments.

Effects of Electrocardiograms QRS Detection Algorithms in Heart Rate Variability Metrics

This study evaluates how different QRS detection algorithms affect HRV metrics across various recording setups and conditions, finding significant variability in performance and recommending context-specific algorithm selection.

SIMBA: an agentic AI platform for single-molecule multi-dimensional imaging

SIMBA is an AI platform that integrates single-molecule imaging analysis with deep learning, enabling automated and interactive processing of multi-dimensional fluorescence data.

Scaling Multiplex qPCR Primer Design to 1000-plex using the Degenerate Incomplete Multiplex Primer List Extension (DIMPLE) Algorithm

The paper introduces DIMPLE, a scalable qPCR primer design algorithm that enables 10,000+ primers in a single reaction, achieving low VAF detection and multiplex applications.

Nanofitin-Engineered Affinity Chromatography for Marker-Defined Extracellular Vesicle Enrichment in Scalable Downstream Processing

This paper develops a Nanofitin-based affinity chromatography method to selectively enrich CD81-positive extracellular vesicles from conditioned medium with high recovery and impurity reduction.

Effects of MRI on an Injectable Hydrogel with Magnetically Alignable Microstructures for Oriented Cell Growth

This study examines the effects of MRI on an injectable hydrogel with magnetic microstructures, finding no change in alignment or heat generation under MRI exposure.

Identification and Optimization of Kratom Strictosidine Pathway Enabled by Yeast Multiplex Engineering

This study identifies and optimizes the kratom strictosidine biosynthetic pathway using yeast multiplex engineering, increasing strictosidine production by 62%.

Structure-Function Analysis of the FCRL5-IgG1 Fc Complex Reveals an Unappreciated Effect of Fc-Silent Antibodies on B cells

This study shows Fc-silencing mutations in therapeutic antibodies do not block FCRL5 binding, potentially affecting B cell function through novel Fc-FCRL5 interactions.

The Mechanical Fingerprint of Hippocampal Sclerosis Linking Neuronal Cell Loss and Gliosis to Tissue Stiffness

This study links neuronal cell loss and gliosis in hippocampal sclerosis to tissue stiffness using mechanical testing and deep learning analysis of histopathology.

Microporous annealed particle scaffolds avoid foreign body response by down regulating complement-fibroblast-macrophage signaling loop

This study compares immune responses to microporous annealed particle scaffolds and nanoporous hydrogels, finding that MAP scaffolds reduce foreign body response by suppressing complement-fibroblast-macrophage signaling.

Beyond Histology: A Validated CUBIC-Based Workflow for Volumetric Analysis of Follicles and Cortical Vasculature in Human Ovarian Tissue

This paper validates a CUBIC-based workflow for volumetric analysis of follicles and vasculature in human ovarian tissue, showing comparable follicle density to histology and detailed vascular morphometrics.

Noncoaxial Transcatheter Aortic Valve Deployment Creates Cusp-Specific Thrombogenic Microenvironments Through Altered Sinus Hemodynamics

This study shows that noncoaxial transcatheter aortic valve deployment creates cusp-specific thrombogenic microenvironments by altering sinus hemodynamics, leading to localized leaflet thrombus formation.

Lipid Tail Length Determines Nano-Bio Interactions of Peptide Amphiphile Nanostructures

This study investigates how lipid tail length in peptide amphiphile nanostructures affects their interactions with biological systems, showing that molecular design can control biointeractions and tumor accumulation.

A 3D in vitro model of the human hepatobiliary junction

This paper develops a 3D in vitro model of the human hepatobiliary junction that recapitulates bile flow and dysfunction under hypoxia-reoxygenation, offering insights into biliary pathophysiology.