M. Castro

Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells

This paper introduces PERC, a method for efficient CRISPR editing in primary human immune cells and HSPCs using a peptide reagent, achieving over 90% editing efficiency without specialized equipment.

Epigenetic reprogramming of autophagy leads to uncovering a novel therapeutic target for mutant IDH1 astrocytomas

This study identifies autophagy as a critical survival pathway in mIDH1 gliomas and shows that inhibiting autophagy enhances radiosensitivity and tumor regression in preclinical models.

Can changes in ploidy drive the evolution to allogamy in a selfing species complex?

The paper investigates how ploidy levels affect mating strategies in a selfing plant species complex, finding that higher ploidies show reduced inbreeding depression and traits associated with outcrossing.

De novo lipogenesis pathway is a vulnerability in IDH1 mutant glioma

This study identifies de novo lipogenesis as a vulnerability in IDH1 mutant gliomas, showing that HDAC inhibition and FASN targeting reduce tumor growth and improve survival.

PAH DEFICIENT PATHOLOGY IN HUMANIZED c.1066-11G>A PHENYLKETONURIA MICE

This paper generates a partially humanized mouse model of PKU with the c.1066-11G>A PAH variant, showing splicing defects, metabolic imbalances, and neurological phenotypes.

Antigen binding kinetics are quite different for B-cell receptors and free antibodies

This paper quantifies how B-cell receptor and antibody antigen binding kinetics differ due to structural and diffusion differences, showing effective rates deviate from intrinsic rates beyond certain thresholds.

Time and spatial trends in landing per unit of effort as support to fisheries management in a multi-gear coastal fishery

The paper analyzes trends in landing per unit of effort (LPUE) for 48 species in a Portuguese coastal fishery to inform regional management plans.

Therapeutic reversal of prenatal pontine ID1 signaling in DIPG

This study identifies ID1 upregulation in DIPG tumor cells and shows that CBD can reduce ID1-driven invasiveness in vitro and in vivo.

Affinity selection in germinal centers: Cautionary tales and new opportunities

The paper applies a mathematical framework to analyze discrepancies in B-cell receptor interactions and retraction forces during germinal center reactions, proposing testable predictions.

DNA Methylation-based Signatures Classify Sporadic Pituitary Tumors According to Clinicopathological Features.

This paper identifies and validates DNA methylation signatures that classify sporadic pituitary tumors based on clinicopathological features and adenohypophyseal cell lineages.

Characteristic and quantifiable COVID-19-like abnormalities in CT- and PET/CT-imaged lungs of SARS-CoV-2-infected crab-eating macaques (Macaca fascicularis)

This study uses CT and PET/CT imaging to quantify SARS-CoV-2-induced lung abnormalities in crab-eating macaques, providing an objective endpoint for evaluating medical countermeasures.

Inhibition of 2-Hydroxyglutrate Elicits Metabolic-reprograming and Mutant IDH1 Glioma Immunity

This study shows that inhibiting 2-hydroxyglutarate in mIDH1 gliomas with ATRX/TP53 inactivation improves survival and induces anti-tumor immunity through immunological memory and reduced T-cell exhaustion.

Pre-TCR signaling intensity shapes the TCRβ repertoire

The paper shows that pre-TCR signaling intensity affects TCRβ repertoire diversity in murine thymocytes by influencing nanoclustering and maturation.

CCR5 deficiency impairs CD4+ T cell memory responses and antigenic sensitivity through increased ceramide synthesis

This study shows that CCR5 deficiency in CD4+ T cells increases ceramide synthesis, reduces TCR nanoclustering, and impairs memory responses and antigenic sensitivity.

Selectivity and engineering of the sialoglycan-binding spectrum in Siglec-like adhesins

This study identifies determinants of sialoglycan-binding selectivity in Siglec-like adhesins and creates engineered binding proteins for specific glycan recognition.

The scaling of genome size and cell size limits maximum rates of photosynthesis with implications for ecological strategies

The paper links genome size to cell size and photosynthesis rates in plants, showing genome size predicts metabolic capacity and ecological strategies.