Max Greenberg

Dual profiling of DNA modifications with enhancer features during the exit of naive pluripotency

The paper uses Methyl-ATAC and Methyl-CUT&Tag to study 5mC and 5hmC dynamics during mESC differentiation, revealing their role in enhancer regulation.

MYC and MAX drive the reactivation of the genome after mitosis

This study identifies MYC and MAX as critical for post-mitotic genome reactivation in pluripotent mouse embryonic stem cells, showing MAX's role in bookmarking and MYC recruitment.

DNA methylation links lagging strand replication to transposable element control

The paper shows DNA methylation maintenance is linked to lagging-strand replication and TE control in mouse cells, with TE integration preferentially occurring on the lagging strand.

DNA methylation shapes the Polycomb landscape during the exit from naïve pluripotency

This study investigates how DNA methylation influences the Polycomb landscape during the exit from naïve pluripotency, finding that 5mC can antagonize H3K27me3 and activate genes in differentiated cells.

The embryonic DNA methylation program modulates the cis-regulatory landscape via CTCF antagonism

This study shows that DNA methylation affects CTCF binding and 3D chromatin structure during mouse embryonic differentiation.

DNA methylation restricts coordinated germline and neural fates in embryonic stem cell differentiation

This study shows that DNA methylation is critical for restricting neural differentiation but not germline fate in ESCs, with TKO ESCs capable of germline specification.

DAXX safeguards heterochromatin formation in embryonic stem cells

This study identifies DAXX's role in maintaining heterochromatin structure in ESCs by recruiting PML and SETDB1, and shows its importance for genome integrity and pluripotency.

A genome-wide knock-out screen for actors of epigenetic silencing reveals new regulators of germline genes and 2-cell like cell state

The paper identifies new regulators of germline genes and 2-cell like cell state through a genome-wide CRISPR knock-out screen in mouse ES cells.

Conservation and divergence of canonical and non-canonical imprinting in murids

The paper identifies imprinted genes in rat tissues, finds conservation of canonical imprints in embryos but divergence in extra-embryonic tissues, and discovers novel non-canonical imprints.

H3K9 tri-methylation at Nanog times differentiation commitment and enables the acquisition of primitive endoderm fate

The paper identifies H3K9me3 at the Nanog locus as a repressive mark that marks differentiation commitment and enables primitive endoderm fate acquisition in ES cells.

Dynamic enhancer partitioning instructs activation of a growth regulator during exit from naïve pluripotency

The paper shows that CTCF-mediated enhancer partitioning controls the promoter switch of Zdbf2 during exit from naïve pluripotency, independent of polycomb and DNA methylation.