Redmond P. Smyth

Aminoacylated tRNA tails trigger auxiliary bacterial immunity

The paper shows that cleaved aminoacylated tRNA tails trigger auxiliary bacterial immunity by activating Adt1, leading to mRNA cleavage and cellular dormancy.

Field-isolate recombinant tick-borne encephalitis viruses define reporter-stability guidelines for antiviral testing in flaviviruses

This paper develops recombinant tick-borne encephalitis viruses with reporter genes to guide antiviral testing, finding that Nluc is more stable than eGFP in certain conditions.

mRNA stability in response to m6A placement is linked to cell identity in planarians

The paper identifies m6A sites in planarians and shows their role in cell-type-specific mRNA stability, with implications for cell identity.

Influenza Virus Infection of an Immunocompetent Organotypic Model of the Human Respiratory Mucosa

The paper develops an immunocompetent 3D model of human respiratory mucosa to study influenza virus infection and immune responses.

Expression Levels of the Attachment Protein G Differ Between Strains of a Murine Pneumovirus and Determine the Virulence

The paper identifies differences in G protein expression levels between PVM strains, linking these differences to virulence through genetic variations in the G gene.

Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species

The paper provides a comprehensive analysis of tRNA modifications in Staphylococcus aureus, revealing species-specific features and decoding dynamics.

Visualizing the transcription and replication of influenza A viral RNAs in cells by multiple direct RNA padlock probing and in-situ sequencing (mudRapp-seq)

This study introduces mudRapp-seq to visualize influenza A viral RNA transcription and replication at the single-cell level, revealing early mRNA expression and differential segment expression.

Demultiplexing and barcode-specific adaptive sampling for nanopore direct RNA sequencing

WarpDemuX improves multiplexing accuracy and speed for nanopore dRNA-seq, enabling efficient phenotyping of SARS-CoV-2 variants and real-time RNA enrichment.

Native epitranscriptome sequencing reveals Hepatitis B virus RNA stability protected by heavy m6A modification

This study uses Nanopore DRS to show m6A modification on HBV RNA enhances stability by protecting long poly(A) tails, supporting m6A as an antiviral target.

RNA genome expansion up to 64 kb in nidoviruses is host constrained and associated with new modes of replicase expression

The paper expands the known range of nidovirus genomes up to 64 kb and identifies host constraints and new replicase expression modes in giant invertebrate nidoviruses.

Sequencing accuracy and systematic errors of nanopore direct RNA sequencing

This study evaluates sequencing accuracy and systematic errors in direct RNA sequencing across species and synthetic RNA, identifying error patterns and their implications for downstream applications.

NEAT1 promotes genome stability via m6A methylation-dependent regulation of CHD4

This study identifies NEAT1 as a regulator of genome stability through m6A methylation-dependent control of CHD4 in response to DNA damage.

Building Blocks of Understanding: Constructing a Reverse Genetics Platform for studying determinants of SARS-CoV-2 replication.

This paper develops a reverse genetics platform for SARS-CoV-2 to generate recombinant viruses and study their replication and phenotypic effects.

Codon affinity in mitochondrial DNA shapes evolutionary and somatic fitness

The paper identifies a synonymous mtDNA variant that affects codon-anticodon affinity and shows its impact on cellular fitness in immune cells.

Revealing the host antiviral protein ZAP-S as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting

The paper identifies ZAP-S as a host protein that inhibits SARS-CoV-2 programmed ribosomal frameshifting, reducing viral replication.