Lars Dölken

Herpes simplex virus infection promotes ALS pathology through ICP0-mediated PML body disruption

This study shows HSV infection increases ALS risk and promotes TDP-43 pathology through ICP0-mediated disruption of PML bodies.

Murine CMV Infection Unmasks Macrophage-Driven Inflammatory Cardiomyopathy in Pkp2, but not in Ttn Mutant Mice

This study shows that murine cytomegalovirus infection exacerbates inflammation in Pkp2 mutant mice but not in Ttn mutant mice, highlighting the interaction between genetic predisposition and environmental factors in cardiomyopathy.

Murine cytomegalovirus evolved a cell-cycle regulator (m54.5) within the highly conserved viral DNA polymerase gene

This study identifies m54.5 as a novel gene within the conserved MCMV DNA polymerase locus that encodes a protein regulating cell cycle arrest, with functional similarity to HCMV UL21a but not essential for viral replication.

Unveiling immune interference: How the dendritic cell response to co-infection with Aspergillus fumigatus is modulated by human cytomegalovirus and its virokine CMVIL-10

This study investigates how HCMV and its virokine CMVIL-10 modulate dendritic cell responses during co-infection with A. fumigatus, revealing that CMVIL-10 has a limited role in suppressing antifungal immunity.

HSV-1 infection induces a downstream-shift of the +1 nucleosome

This study shows HSV-1 infection causes a downstream shift of +1 nucleosomes by depleting RNA Polymerase II from host genes, similar to Pol II depletion in yeast.

Identifying Modulators of Cellular Responses by Heterogeneity-sequencing

Heterogeneity-seq identifies causal factors driving heterogeneous cellular responses by combining single-cell RNA-seq with metabolic labeling and double machine learning.

Herpes simplex virus 1 inhibits phosphorylation of RNA polymerase II CTD serine-7

This study shows HSV-1 inhibits phosphorylation of RNA polymerase II CTD serine-7, affecting viral gene expression and host transcription.

Correcting 4sU induced quantification bias in nucleotide conversion RNA-seq data

The paper develops computational methods to correct 4sU-induced quantification bias in nucleotide conversion RNA-seq data by addressing mappability and reverse transcription efficiency issues.

Building Blocks of Understanding: Constructing a Reverse Genetics Platform for studying determinants of SARS-CoV-2 replication.

This paper develops a reverse genetics platform for SARS-CoV-2 to generate recombinant viruses and study their replication and phenotypic effects.

mRNA N1-2'O-methylation by CMTR1 affects NVL2 mRNA splicing

This study shows that CMTR1-mediated N1-2'O-methylation prevents antiviral recognition and regulates NVL2 mRNA splicing, affecting cell size, division, and ribosome biogenesis.

HSV-1 infection induces a downstream shift of promoter-proximal pausing for most host genes

This study shows HSV-1 infection causes a downstream shift in promoter-proximal Pol II pausing for most host genes, more pronounced than NELF depletion.

The Cytomegalovirus M35 Protein Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes by Direct Promoter Binding

This paper identifies M35 as a viral protein that binds DNA and inhibits IRF3-mediated gene expression, including Ifnb1, thereby suppressing the antiviral type I IFN response.

HSV-1 and influenza infection induce linear and circular splicing of the long NEAT1 isoform

The paper shows HSV-1 and influenza infection induce circular and linear splicing of the NEAT1_2 isoform, independent of vhs and linked to transcription termination disruption.

U1 snRNP-dependent suppression of miRNA biogenesis by alternative intronic polyadenylation in melanoma

The paper shows that U1 snRNP-dependent intronic polyadenylation suppresses miRNA biogenesis in melanoma by altering TRPM1 alternative polyadenylation signals.

The zinc finger antiviral protein ZAP destabilises viral transcripts and restricts cytomegalovirus

This study shows that ZAP restricts HCMV infection by destabilizing viral transcripts with low CG content, revealing its role in antiviral defense against DNA viruses.

Mechanism and consequences of herpes simplex virus 1-mediated regulation of host mRNA alternative polyadenylation

This study shows HSV-1 infection causes widespread changes in host mRNA alternative polyadenylation, with ICP27 playing a role in shifting polyadenylation to intronic sites and affecting mRNA export and expression.

scSLAM-seq reveals core features of transcription dynamics in single cells

scSLAM-seq measures transcription dynamics in single cells by distinguishing newly synthesized from pre-existing RNA, revealing stochastic transcription and gene-specific differences.