April Pawluk

Systematic mapping of the prokaryotic noncoding sequence space with a genomic language model

The paper uses a genomic language model to cluster and annotate noncoding sequences in prokaryotic genomes, identifying structured RNAs and their potential functions.

The adaptive architecture of tRNA dependencies across physiological tumor environments

The paper maps tRNA dependencies in cancer cells across different environments, finding that tRNA dependencies are environmentally sensitive while some dependencies are shared across cell lines.

Extracellular cGAMP transmission exacerbates neuroinflammation in aging and multiple sclerosis

This study shows that extracellular cGAMP worsens neuroinflammation in aging and MS by activating STING in microglia, astrocytes, and inhibitory neurons, with ENPP1 acting as a protective brake.

A combinatorial domain screening platform reveals epigenetic effector interactions for transcriptional perturbation

The paper introduces COMBINE, a platform that screens epigenetic effector domain pairs to identify synergistic and antagonistic interactions, revealing new mechanisms for transcriptional regulation.

Site-specific DNA insertion into the human genome with engineered recombinases

This study engineers recombinases to improve DNA insertion efficiency and specificity in human cells, achieving 53% integration and 97% genome-wide specificity.

Rewriting endogenous human transcripts with trans-splicing

This paper introduces RESPLICE, a CRISPR-based trans-splicing system that efficiently edits endogenous human transcripts, achieving up to 90% trans-splicing efficiency in sorted cells.

Bridge RNAs direct modular and programmable recombination of target and donor DNA

The paper demonstrates that IS110 insertion sequences use a structured RNA to direct sequence-specific DNA recombination, enabling modular genome editing.

Potent Cas9 inhibition in bacterial and human cells by new anti-CRISPR protein families

The paper identifies and characterizes two new anti-CRISPR protein families that inhibit type II-C Cas9 systems, showing potent inhibition of NmeCas9 and cross-species activity.