John S. Gilleard

Development, field-testing and optimization of tools to quantify soil-transmitted helminths in fecal sludge from school pit latrines in Ethiopia

The paper develops and tests tools to quantify soil-transmitted helminth eggs in fecal sludge from school pit latrines in Ethiopia, finding that latrine-based surveys can be as precise as stool-based surveys for certain parasites.

The development and validation of long-read ITS-1/5.8S/ITS-2 nemabiome metabarcoding using Oxford Nanopore Technologies (ONT) sequencing for ovine gastrointestinal nematodes and the design of primers suitable for its direct application to fecal DNA.

Using deep amplicon sequencing as a molecular xenomonitoring approach for detecting filarial nematodes in biting arthropod vectors

This paper develops a deep amplicon sequencing method to detect multiple filarial nematode species in mosquito pools, showing high accuracy but species-specific read biases.

Over-expression and increased copy numbers of a cytochrome P450 and two UDP-glucuronosyltransferase genes in macrocyclic lactone resistant Psoroptes ovis of cattle

The paper identifies over-expression and copy number variation of cytochrome P450 and UDP-glucuronosyltransferase genes in ML-resistant Psoroptes ovis mites from cattle.

Naturally occurring variation in a cytochrome P450 modifies thiabendazole responses independent of beta-tubulin

This paper identifies a cytochrome P450 gene variant that contributes to benzimidazole resistance in nematodes, independent of beta-tubulin mutations.

Discovery of a novel Trichuris species, T. incognita, with low sensitivity to albendazole-ivermectin treatment in Cote d'Ivoire patients, identified through fecal DNA metabarcoding

Molecular evidence of widespread benzimidazole drug resistance in Ancylostoma caninum from domestic dogs throughout the USA and discovery of a novel isotype-1 β-tubulin benzimidazole resistance mutation.

This paper identifies widespread benzimidazole resistance in Ancylostoma caninum from dogs across the USA, including a novel β-tubulin mutation and confirms its functional significance.

Genomic signatures of selection associated with benzimidazole drug treatments in Haemonchus contortus field populations

This study identifies genomic signatures of benzimidazole drug resistance in Haemonchus contortus using field populations with defined treatment histories, showing the isotype-1 β-tubulin gene as the primary resistance locus.

Interactions of C. elegans -tubulins with the microtubule inhibitor albendazole

The paper investigates the role of C. elegans β-tubulin genes in albendazole resistance, finding that tbb-2 modifies sensitivity and ben-1 mutations primarily confer resistance.

Genomic landscape of drug response reveals novel mediators of anthelmintic resistance

The paper maps resistance loci for three anthelmintic classes in a parasitic nematode, identifies novel alleles for benzimidazoles and levamisole, and implicates cky-1 in ivermectin resistance.

Multiple drug resistance in hookworms infecting greyhound dogs in the USA

This study identifies high prevalence and intensity of multiple-drug-resistant Ancylostoma caninum in greyhound dogs in the USA, with strong genetic and phenotypic evidence of resistance.

Extensive genomic and transcriptomic variation defines the chromosome-scale assembly of Haemonchus contortus, a model gastrointestinal worm

The paper presents a high-quality, chromosome-scale genome assembly of Haemonchus contortus using long-read sequencing, revealing genetic diversity and transcriptional regulation.

A database for ITS2 sequences from nematodes

The paper presents a database of ITS2 sequences from nematodes and the software used to build it, along with tools to create ITS2 databases for other taxonomies.

Evidence for the F200Y (TAC) mutation conferring benzimidazole resistance in a southern USA cattle population of Haemonchus placei spreading from a single emergence

Population genomic and evolutionary modelling analyses reveal a single major QTL for ivermectin drug resistance in the pathogenic nematode, Haemonchus contortus

The paper identifies a single major QTL on chromosome V associated with ivermectin resistance in Haemonchus contortus through genome-wide analysis of genetic crosses.

Comparative genomics of the major parasitic worms

This paper compares genomes of 81 nematode and platyhelminth species to identify gene families related to parasitism and prioritize new drug targets.

A genome resequencing-based genetic map reveals the recombination landscape of an outbred parasitic nematode in the presence of polyploidy and polyandry

This paper constructs a genetic map of Haemonchus contortus using whole-genome resequencing, revealing recombination patterns and reproductive traits like polyandry and polyploidy.