genetic perturbation / CRISPR

This study identifies TMEM55B as a regulator of lysosomal acidification and ER-lysosome calcium signaling, which promotes lipolysis and cholesterol metabolism.

This study shows that engineered NKG2C+ NK-like T cells have better antitumor effects and lower cytokine release syndrome risk than conventional CAR-T and CAR-NK cells.

This paper shows that loss-of-function variants in MAP3K7 cause dilated cardiomyopathy (DCM), both as part of syndromic disease and as apparently isolated DCM.

This study identifies a novel regulatory mechanism for the LytM/M23 family endopeptidase LmdC in P. aeruginosa, showing it is auto-cleaved and degraded by proteases Prc and CtpA, with inhibition by Baf and Api.

This study identifies centromeric structural variants associated with molecular traits and complex diseases in humans using long-read sequencing and haplotype-resolved analysis.

This study identifies host determinants for phage infection in Mycobacterium smegmatis using CRISPR interference, revealing receptor proteins and lipid components involved in phage entry.

This study identifies outer membrane porins as phage-specific determinants of mycobacterial susceptibility through a CRISPRi screen in Mycobacterium smegmatis.

The paper identifies chromatin-opening transcription factors in mouse embryonic stem cells using CRISPRa screens, revealing autonomous, opportunistic, and hierarchical mechanisms.

The paper develops an integration-free CRISPR platform to study host dependencies of respiratory viruses in human nasal epithelium, showing ACE2 and TMPRSS2 are critical for SARS-CoV-2 and hCoV-NL63 infection.

This paper develops CRISPRi-based inducible systems to fine-tune antibiotic susceptibility in Mycobacterium tuberculosis by targeting key genes involved in drug activation.

This study identifies MgpB and CorB as new mediators of magnesium homeostasis in Salmonella, showing they reduce intracellular Mg2+ and protect against excess-cation stress.

The paper identifies a hydrolysis operon in Lachnospiraceae that enables castalagin metabolism to urolithins and flavogallonic acid in the human gut microbiome using multi-omics analysis of fecal samples from a clinical trial.

This study identifies how Yersinia pseudotuberculosis uses YopE and YopH to inhibit neutrophil degranulation and ROS production through distinct pathways.

This paper identifies DD-CPases as the source of tetrapeptides for LDTs in C. difficile and shows that LDTs are essential due to high DD-CPase activity, not for divisome/elongasome function.

This study uses a CRISPR screen to identify genes critical for erythroblast enucleation, finding CLIC3 and VAMP8 as key factors with distinct roles in the process.

This study identifies the CrdRS two-component system as a key regulator of antibiotic-induced biofilm formation in H. pylori through bidirectional control of transporters PlpA and GlnP.

This study shows that targeting catecholate siderophore systems reduces AIEC colonization in colitis by immunization, preserving gut microbiome composition.

This study identifies tumor-intrinsic stress states as modulators of CAR T cell therapy response in pancreatic cancer using CRISPR screens and single-cell genomics.

This study identifies sustained cell cycle activity as a mechanism of resistance to KRAS inhibitors and shows that co-targeting cell cycle nodes with CDK4/6 or CDK2 restores sensitivity and provides durable tumor control.

The paper repurposes marine bacteria to convert lignin-derived carbon into valuable chemicals using seawater, achieving carbon and water conservation.

This study shows that platelet PAR4 signaling, interacting with estrogen receptor beta, drives glioblastoma progression and immune suppression, with sex-specific effects on survival and TME dynamics.

This study shows that blocking TGFβ signaling in TCR-engineered T cells and enhancing antigen processing in tumor cells improves their therapeutic effectiveness against pancreatic ductal adenocarcinoma.

The paper identifies SamI as a cytosolic SPFH protein that couples membrane stress to differentiation in Bacillus subtilis by stabilizing a membrane complex involved in stress signaling and biofilm formation.

The paper re-annotates and characterizes the DGF-1 gene family in T. cruzi, revealing its modular structure and evolutionary history across trypanosomatids.

This study identifies ZNF865 as a regulator of cell senescence and DNA damage in intervertebral discs, showing its role in back pain and potential as a therapeutic target.

This study identifies C3aR as a mediator of HFD-induced hypothalamic microgliosis and obesity, showing its role in lipid droplet formation and neuroinflammation.

This paper introduces a geometric framework to identify coherent structures in active flows on dynamic surfaces, revealing new transport patterns in biological systems.

This study shows Greatwall kinase regulates AML cell division through a non-canonical pathway distinct from its role in solid tumors.

This study uses CRISPR/Cas9 to suppress lachrymatory factor synthase in onions, reducing tear-inducing activity but causing growth issues.

This paper develops a CRISPRa-based method to identify genes enhancing CAR T cell survival in different tumor environments.