flow cytometry

Use of human AML cells to study graft-versus-leukemia immunity in xenogeneic mouse models of GVHD

The paper describes methods for studying graft-versus-leukemia immunity in xenogeneic mouse models using human AML cells and PBMCs.

The differential immunological impact of photon vs proton radiation therapy in high grade lymphopenia

This study compares the immunological effects of photon and proton radiation therapy in cancer patients, finding that proton therapy preserves T cell diversity and may improve survival.

Investigating Cell Viability under Shear Stress in Complex Microstreaming Flows Generated by Ultrasound-Driven Actuated Microbubbles

This paper introduces a method using ultrasound-driven microbubbles to manipulate and sort human cells in microfluidic channels while maintaining cell viability.

Long-term immune changes after COVID-19 and the effect of BCG vaccination and latent infections on disease severity

This study examines long-term immune changes post-COVID-19 and the impact of BCG vaccination and latent infections on disease severity.

Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils.

This study develops an in vitro differentiation and cell sorting method to isolate highly pure mouse basophils (>98%) that maintain lineage markers and functional responses.

FLOW CYTOMETRIC PROCEDURES FOR DEEP CHARACTERIZATION OF NANOPARTICLES

This paper optimizes flow cytometry procedures to accurately identify, quantify, and purify extracellular vesicles and virus-like particles by reducing artifacts and improving specificity.

Deterministic Cell Pairing with Simultaneous Microfluidic Merging and Sorting of Droplets

This paper introduces a microfluidic platform that deterministically pairs and sorts droplets containing cells, achieving high recovery of target cell pairs for studying cell crosstalk.

Interaction with tumor cell spheroids induces suppression in primary human cytotoxic T cells

The paper generates and characterizes primary human cytotoxic T cells with suppressed function in vitro, showing their resemblance to exhausted tumor-infiltrating CTL and identifying factors affecting their effector function.

Expression of IL-1R2 by Tfr prevents their IL-1-dependent proliferation and the exacerbation of allergy

This study shows that IL-1R2 on Tfr cells prevents excessive activation and allergy by limiting IgE and promoting IgG responses.

Impact of cryopreservation on immune cell metabolism as measured by SCENITH.

This study uses SCENITH to show cryopreservation affects immune cell metabolism, favoring glycolysis and impairing oxidative phosphorylation, but still allows for distinct metabolic profiling of cell subsets.

CD6 Regulates CD4 T Follicular Helper Cell Differentiation and Humoral Immunity During Murine Coronavirus Infection

This study shows CD6 suppresses CD4 T cell activation and follicular helper cell differentiation during viral infection, reducing antiviral humoral immunity.

Stepwise Isolation of Diverse Metabolic Cell Populations Using Sorting by Interfacial Tension (SIFT)

The paper introduces a label-free microfluidic platform to sort cells based on glycolytic activity by measuring pH changes, enabling the isolation of distinct metabolic cell populations.

Seasonal influenza A virus lineages exhibit divergent abilities to antagonize interferon induction and signaling

This study compares the ability of seasonal H1N1 and H3N2 influenza A viruses to antagonize interferon induction and signaling at the single-cell level, finding differences in their IFN antagonism potential.

Enhancing reproducibility and decentralization in single cell research with biocytometry

This study evaluates biocytometry's performance in a decentralized setting, showing its ability to accurately count rare cells across multiple labs with minimal training.

Donor HLA DQ reactive B cells clonally expand under chronic immunosuppression and include atypical CD21lowCD27- B cells with high avidity germline B cell receptors.

CD4 Co-Receptor Regulates Sex-Specific NK Cell Responses to Acute Toxoplasma gondii Infection.

This study shows that CD4 co-receptor deficiency leads to faster death in female mice infected with T. gondii due to reduced IFN-gamma+ NK cells, but not in males.

Ex-vivo expansion of patient-derived PBMCs preferentially results in effector memory T-cell proliferation with restored autologous efficiency in HBV-HCC

Transitioning to e-cigarettes restores the immune response to house dust mite in cigarette smoked mice.

This study shows that short-term e-cigarette use after cigarette smoke exposure restores immune response to house dust mites, but long-term use may not fully restore immunity.

The consequences of mismatched buffers in spectral cell sorting

The paper shows that mismatched buffers in spectral cell sorting can lead to incorrect purity check results due to data unmixing issues.

Development and utilization of Treponema pallidum expressing green fluorescent protein to study spirochete-host interactions and antibody-mediated clearance: expanding the toolbox for syphilis research.

The paper develops a fluorescent Treponema pallidum strain to study spirochete-host interactions and antibody-mediated clearance, showing greater OM disruption with sera from rabbits infected with the Nichols strain.

Quantifying synthetic bacterial community composition with flow cytometry: efficacy in mock communities and challenges in co-cultures

This study evaluates flow cytometry's ability to quantify synthetic bacterial communities, showing it outperforms PCR-based methods in mock communities but faces challenges in co-cultures due to phenotypic changes.

Neutrophils drive sexual dimorphism in experimental periodontitis

This study investigates sex-specific differences in periodontitis progression, finding that neutrophil inflammation drives sexual dimorphism in bone loss.

Distinct immune profiles in children living with HIV based on timing and duration of suppressive antiretroviral treatment

This study compares immune profiles of children living with HIV who started antiretroviral treatment at different times and durations, finding distinct immune differences between early and delayed treatment groups.

Tetanus Toxoid Utilizes Dual-Faceted Anticancer Mechanism Through Targeting Tumoral Sialic Acids and Enhancing Cytotoxic CD4+ T cell Responses Against Pancreatic Cancer

This paper shows that tetanus toxoid (TT) reduces pancreatic cancer by boosting cytotoxic CD4+ T cells and targeting sialic acids on tumor cells.

Detritus identification in FlowCAM using a simple binary classifier

This paper uses logistic regression to classify detritus particles in FlowCAM data from the Southern North Sea, achieving 81% accuracy and improving pre-processing efficiency.

Sigma Ligand-mediated Cytosolic Calcium Modulation in BV2 Microglia Cells

This study investigates how sigma receptor ligands modulate cytosolic calcium levels in BV2 microglia cells, finding increased calcium levels and extracellular calcium entry with certain ligands.

Chronic Viral Reactivation and Associated Host Immune Response and Clinical Outcomes in Acute COVID-19 and Post-Acute Sequelae of COVID-19

This study investigates viral reactivation in COVID-19 patients, finding associations between reactivation of specific virus families and clinical outcomes, including mortality and PASC symptoms.

Rapid and precise quantification of lymphocyte iron content by single cell inductively coupled plasma mass spectrometry

The paper quantifies single-cell iron content in lymphocytes using ICP-MS and links iron levels to cellular activation and function.

Distinct neutrophil effector functions in response to different isolates of Leishmania aethiopica

The paper investigates how different Leishmania aethiopica isolates affect neutrophil effector functions in vitro, finding that long-term cultured isolates induce more ROS production and distinct neutrophil populations.

The universal labeling and sorting of avian primordial germ cell utilizing Lycopersicon Esculentum lectin

This paper introduces a method using Lycopersicon Esculentum lectin to label and sort primordial germ cells in avian species, showing high efficiency and subtype identification.