flow cytometry

The reparative immunologic consequences of stem cell transplantation as a cellular therapy for refractory Crohn's disease

This study investigates how stem cell transplantation (SCT) restores immune function in refractory Crohn's disease by analyzing immune cell remodeling and HSC heterogeneity using single-cell RNA sequencing and functional assays.

Lung CD4+ resident memory T cells use airway secretory cells to stimulate and regulate neutrophilic allergic airways disease.

This study identifies lung CD4+ resident memory T cells and their interaction with airway secretory cells as key regulators of neutrophilic allergic airways disease in mice and humans.

Characterisation of CD4 Th subsets as well as dietary, exercise and lifestyle factors in an established Rheumatoid Arthritis cohort; pilot study.

This study examines sex-specific differences in CD4 Th subsets and lifestyle factors in a RA cohort, finding sex-related variations in Th cell frequencies and dietary patterns.

Automated Cytometric Gating with Human-Level Performance Using Bivariate Segmentation

This paper introduces UNITO, a deep learning framework for automated cytometric gating that matches human performance and provides interpretable results without requiring manual input.

MAGE-A4-Responsive Plasma Cells Promote Non-Small Cell Lung Cancer

The paper shows MAGE-A4 promotes NSCLC by recruiting IgA+ plasma cells, reducing tumor burden and enhancing T cell activity.

Splenic MARCO+ marginal zone macrophages regulate rapid production of MCP-1 and KC but are dispensable for alloantibody generation in response to stored RBCs in a murine model.

This study shows splenic MARCO+ marginal zone macrophages (MZMs) enhance cytokine production but are not required for anti-RBC alloantibody generation after stored RBC transfusion in mice.

Regulatory T and activated T cells in the chronic unpredictable mild stress mice: sex differences and effects of treatments

This study examines the effects of antidepressants on immune responses and depressive behaviors in male and female CUMS mice, finding sex differences in T-cell activation and immune regulation.

Cancer cells suppress NK activity by actin-driven polarisation of inhibitory ligands at the synapse

This study shows cancer cells evade NK cell cytotoxicity by polarizing inhibitory ligands at the synapse via actin remodeling.

Investigation of immune response to Mesenchymal Stromal Cell-derived Extracellular Vesicles in the cancer setting

This study investigates whether MSC-EVs elicit an immune response in tumor-bearing mice and finds no significant immune activation, supporting their immune privilege and therapeutic potential.

Unconventionally primed type 1 follicular helper T cells are required for long-lived IgA plasma cell development following mucosal viral infection

This study identifies unconventionally primed TFH1 cells as essential for generating IgA+ long-lived plasma cells during mucosal viral infections.

Schistosoma mansoni-induced host immunopathology is gut microbiota dependent

This study shows that gut microbiota influences liver pathology caused by Schistosoma mansoni infection in mice, but not ileum inflammation.

Integrative deep immune profiling of the elderly reveals systems-level signatures of aging, sex, smoking, and clinical traits

The paper profiles the immune system of elderly individuals and identifies systems-level signatures of aging, sex, smoking, and clinical traits using high-dimensional flow cytometry and multi-block analysis.

Timing anti-PD-L1 checkpoint blockade immunotherapy to enhance tumor irradiation

This study shows that timing anti-PD-L1 therapy after radiation enhances tumor response by preventing CTL depletion and improving PD-L1 blockade.

CD271 sorting for improved liver cell isolation: Semiautomated and simultaneous preparation of parenchymal and non-parenchymal cells from mouse and human livers

This paper introduces a semi-automated protocol for isolating multiple liver cell types from mouse and human livers with high yield, purity, and viability.

CAR T cell engineering impacts antigen-independent activation and co-inhibition

This study compares viral and non-viral methods for CAR T cell engineering, finding that non-viral SB transposition alters T cell activation and co-inhibition markers, with CAR design and donor type influencing outcomes.

SLAMF6 enables efficient attachment, synapse formation, and killing of HIV-1-infected CD4+ T cells by virus-specific CD8+ T cells

This study shows that SLAMF6 is essential for CD8+ T cells to form synapses and kill HIV-1-infected CD4+ T cells, as blocking SLAMF6 reduces conjugate formation and cytotoxicity.

Protocols for circulating neutrophil depletion in neonatal C57Bl/6 mice

The paper tests four neutrophil depletion protocols in neonatal mice, finding that the P3 Dual protocol achieves the best neutrophil clearance with minimal ethical concerns.

Using high-dimensional immunophenotyping for mapping immune cell composition in rapid cryopreserved liquid biopsies

This study develops a 37-marker spectral flow cytometry assay for high-dimensional immunophenotyping of cryopreserved liquid biopsies and validates its robustness against cryopreservation effects.

Differentiation of human T follicular helper cells in vitro requires co-operation between STAT3 and SMAD signaling cytokines that is restrained by IL-2

This study shows that human T follicular helper cells differentiate in vitro through STAT3 and SMAD cytokine cooperation, but IL-2 inhibits this process.

Initiation of primary T cell--B cell interactions and early antibody responses in an organized microphysiological model of the human lymph node

This study develops an in vitro lymph node microphysiological system to model early T cell-B cell interactions and antibody responses using primary human lymphocytes.

Enforced E-selectin ligand installation enhances homing and efficacy of adoptively transferred T cells

This study shows that exofucosylation of T cells enhances their homing and therapeutic efficacy in treating solid tumors and metastases.

30-Color Longitudinal Full-Spectrum Immunophenotyping and Sorting of Human Circulating Immune Cell Subsets Implicated in Systemic Autoimmune Rheumatic Diseases

The paper develops a 30-color immunophenotyping panel to identify and sort immune cell subsets in autoimmune diseases using minimal samples and high resolution.

CD20+ natural killer cells are polyfunctional, memory-like cells that are enriched in inflammatory disorders

This study identifies CD20-expressing NK cells with enhanced functionality, memory-like features, and enrichment in inflammatory diseases.

Hypoxia-Mediated Molecular Interactions of Tissue-Specific Mesenchymal Stem Cells Drive Metabolic Reprogramming and Immunomodulation in Acute Graft-versus-Host Disease

This study compares the immunomodulatory effects of hypoxia-preconditioned bone marrow and Wharton's jelly mesenchymal stem cells in acute graft-versus-host disease, finding WJ-MSCsHYP more effective due to enhanced mitochondrial transfer and metabolic reprogramming.

RhCMV Expands CCR5 Memory T Cells and promotes SIV reservoir seeding in the Gut Mucosa

Maternal type 2 immunity promotes a microchimeric transfer of systemic type 2 immunity to offspring.

This study shows maternal type 2 immunity, independent of antigen exposure, transfers protective immunity to offspring via microchimeric cells.

EARLY NEUTROPHIL ACTIVATION AND NETs RELEASE IN THE PRISTANE-INDUCED LUPUS MICE MODEL

This study shows pristane-induced lupus mice have increased activated neutrophils, LDGs, and NETs compared to controls, suggesting early innate immune activation contributes to lupus development.

Circulating PD-1hi CXCR5- and CXCR5+ CD4 T cells are elevated in patients with newly diagnosed Giant Cell Arteritis and predict relapse

The paper examines circulating PD-1hi CXCR5- and CXCR5+ CD4 T cells in newly diagnosed GCA patients and finds they predict relapse.

Immune correlates of early clearance of Mycobacterium tuberculosis among tuberculosis household contacts in Indonesia

The paper investigates immune correlates of early Mtb clearance in TB household contacts, finding that BCG vaccination is linked to enhanced innate immune responses rather than adaptive immunity.

Quantitative fluorescent nanoparticle tracking analysis and nano-flow cytometry enable advanced characterization of single extracellular vesicles

This paper standardizes fluorescent analysis of extracellular vesicles using nanoparticle tracking analysis and nano-flow cytometry with reference materials, enabling accurate quantification of marker abundance and heterogeneity.