flow cytometry

Novel wheat germ agglutinin-based mass cytometry cell barcoding reagent for heterogeneous, live or fixed sample

This paper develops a wheat germ agglutinin-based barcode for mass cytometry that works on both live and fixed cells, showing stable yields and no differential performance across PBMC lineages.

The Extracellular Matrix Limits Nanoparticle Diffusion and Cellular Uptake in a Tissue-Specific Manner

This study investigates how tissue-specific ECM properties affect nanoparticle diffusion and cellular uptake, finding that PEGylation strategy impacts ECM penetration and uptake differently across tissues.

Automated and closed clinical-grade manufacturing protocol produces potent NK cells against neuroblastoma cells and AML blasts

This paper describes a GMP-compliant protocol for producing potent NK cells against AML and neuroblastoma using an automated platform, showing increased activation and killing efficacy.

45-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of the Major Lineages Present in Human Peripheral Blood Mononuclear Cells with Emphasis on the T cell Memory Compartment.

This paper develops a 45-color flow cytometry panel for high-resolution immunophenotyping of human peripheral blood mononuclear cells, with a focus on T cell memory subsets, demonstrating improved resolution and functional characterization.

Overcoming fixation and permeabilization challenges in flow cytometry by optical barcoding and multi-pass acquisition

This paper introduces a multi-pass flow cytometry technique that enables sequential analysis of cells before and after fixation/permeabilization, improving measurement accuracy for fragile biomarkers.

Development of a Spectral Flow Cytometry Analysis Pipeline for High-Dimensional Immune Cell Characterization

The paper develops a spectral flow cytometry analysis pipeline to identify age-related differences in T cell subsets using high-dimensional data from young and aged mice.

Temporal dynamics of inflammatory and transcriptome changes in the myocardium in a murine model of cardiac arrest

This study examines inflammatory and transcriptomic changes in the myocardium of mice after cardiac arrest, finding increased neutrophils and cytokines that resolve over time, correlating with improved cardiac function.

Quantitative Magnetic Flow Cytometry in High Hematocrit Conditions for Point-of-Care Testing

This paper develops a streamlined magnetic flow cytometry workflow for high hematocrit conditions, achieving reliable cell quantification with low variability.

Hemin-induced platelet activation is regulated via ACKR3 chemokine surface receptor - implications for passivation of vulnerable atherosclerotic plaque

This study shows hemin activates platelets and promotes thrombosis, which can be inhibited by ACKR3 agonists, suggesting a potential therapeutic strategy for atherosclerotic plaque vulnerability.

CD4 downregulation precedes Env expression and protects HIV-1-infected cells from ADCC mediated by non-neutralizing antibodies

The paper shows CD4 downregulation precedes Env expression in HIV-1-infected cells, making them resistant to non-neutralizing antibody-mediated ADCC.

Early and Delayed STAT1-Dependent Responses Drive Local Trained Immunity of Macrophages in the Spleen

This study identifies early and delayed STAT1-dependent responses in splenic macrophages that drive trained immunity, enhancing protection against Salmonella Typhimurium infection.

Relation of CMV and brain atrophy to trajectories of immunosenescence in diverse populations

The paper examines immunosenescence trajectories in diverse populations, finding associations between immunological age and brain atrophy, while highlighting CMV status and genetic ancestry as key factors.

Advancing Adoptive Cell Therapy: Optimized Expansion of Adaptive NK Cells for Solid Tumors

This study shows that IL-21 enhances aNK cell expansion and cytotoxicity against tumors, with tumor lysate boosting their efficacy, validated in a zebrafish model.

Mtb specific HLA-E restricted T cells are induced during Mtb infection but not after BCG administration in non-human primates and humans

Analyzing functional heterogeneity of effector cells for enhanced adoptive cell therapy applications

The paper demonstrates the use of double-emulsion droplet technology to analyze single-cell effector function in adoptive cell therapy, enabling high-resolution study of cell-cell interactions and functional heterogeneity.

ICOS limits memory-like properties and function of exhausted PD-1+ CD8 T cells

This study shows ICOS limits the function of exhausted PD-1+ CD8 T cells by promoting their differentiation into less functional effector-like cells, and inhibiting ICOS improves anti-tumor responses.

42-parameter mass cytometry panel to assess cellular and functional phenotypes of leukocytes in bronchoalveolar lavage of Rhesus macaque

The paper develops a 42-parameter mass cytometry panel for immunophenotyping leukocytes in rhesus macaque bronchoalveolar lavage, identifying multiple cell types and functional markers.

Bovine dendritic-cell subsets in lymphoid and non-lymphoid tissues: work in progress

The paper investigates bovine dendritic-cell subsets in various tissues using flow cytometry and bulk RNA-seq, identifying potential cDC1, cDC2, and pDC subsets based on CD4 and CD13 expression.

Ipsilateral or contralateral boosting of mice with mRNA vaccines confers equivalent immunity and protection against a SARS-CoV-2 Omicron strain

This study shows that boosting mice with mRNA vaccines in the ipsilateral or contralateral arm leads to equivalent immune responses and protection against SARS-CoV-2 Omicron.

Expression of XCR1, CX3CR1, CSF1R and ADGRE1 on subsets of bovine blood dendritic cells and monocytes

The paper examines the expression of XCR1, CX3CR1, CSF1R, and ADGRE1 on bovine blood dendritic cell and monocyte subsets using flow cytometry and validates findings with bulk RNA-seq data.

SIRPalpha+ PD-L1+ bone marrow macrophages aid AML growth by modulating T cell function

The paper identifies a subpopulation of bone marrow macrophages that support AML growth by inhibiting T cells and are enriched in patients with early relapse.

Spectral algal fingerprinting and long sequencing in synthetic algal-microbial communities

This paper introduces a spectral fingerprinting method using full-spectrum cytometry to analyze synthetic algal-microbial communities, showing improved accuracy in species identification and community tracking.

Time of day of infection shapes development of a eukaryotic algal-Nucleocytoviricota virocell

This study shows that the time of day a virus infects an algal host affects viral particle production and bloom dynamics.

An in vitro modelling of resolving macrophage with Raw 264.7 macrophage cell line

This study establishes an in vitro model using Raw 264.7 macrophage cells to simulate resolving macrophage polarisation, showing sequential shifts from M1-like to M2-like to Mres-like phenotypes.

Deciphering the Preeclampsia-Specific Immune Microenvironment: The Role of Pro-inflammatory Macrophages

This study identifies pro-inflammatory macrophages as key regulators of the PE-specific immune microenvironment, linking them to Th17 and CD8+ T cell activation and gMDSC suppression in placental tissues.

Chromatin and transcriptional adaptation accompany monocyte engraftment in the injured retina

The paper examines how engrafted monocytes adapt chromatin and transcriptionally in the injured retina, showing shared and distinct features with host microglia.

A Comparative assessment of T-Cell response of Healthy donors and Acute Graft-versus-Host-Disease Patients: Customizing immune monitoring platform

This study compares T-cell responses between healthy donors and aGVHD patients to optimize PHA and IL-2 concentrations for in vitro T-cell proliferation studies.

Immunophenotyping of T cells combined with Vβ antibodies identifies long lasting CMV related T cell expansions with a consistent TIGIT and PD-1 phenotype

The paper develops a flow cytometry method to analyze T cell receptor Vβ and phenotype simultaneously, identifying long-lasting CMV-related T cell expansions with consistent TIGIT and PD-1 expression.

PIP-Seq identifies novel heterogeneous lung innate lymphocyte population activation after combustion product exposure

The paper introduces PIP-Seq to study lung ILC activation after exposure to environmental toxins, identifying novel ILC subsets and activation pathways.

Assessing male reproductive investment in Papaveraceae using flow cytometry reveals lineage-specific trajectories to pollen-to-ovule ratio reduction

The paper develops a flow cytometry protocol to quantify pollen in Papaveraceae species, revealing lineage-specific patterns in pollen-to-ovule ratio reduction.