Bacterial Genetics and Biotechnology

This study identifies key RNA-binding residues in E. coli ProQ's FinO domain through genetic and biochemical assays, revealing conserved and variable residues involved in RNA interactions.

This study develops a molecular toolkit for studying gene expression and regulation in Acinetobacter baumannii by adapting an E. coli system and introducing a CRISPRi-based gene knockdown method.

The paper describes the IEE protein, which facilitates excision of IS3 family transposons and may immobilize antibiotic resistance genes by removing flanking IS copies.

The paper shows that RicT forms a stable complex with Rny, requiring RicA and RicF, and that iron-sulfur clusters are essential for this interaction, suggesting a functional role for the RicT-Rny complex in mRNA maturation.

The paper uses a cell-free TX-TL assay to identify ASOs targeting MicF sRNA, showing synergistic antibiotic sensitivity reduction when targeting multiple regions.

This study characterizes the organization of Borrelia burgdorferi's segmented genome and interactions between replicons using Hi-C, revealing chromosome structure and plasmid interactions.

The paper shows that non-cognate response regulators sequester phosphorylated histidine kinases, establishing a threshold for bacterial two-component signaling to prevent over-response to weak signals.

The paper describes a protocol for replicating arrayed bacterial mutant libraries to improve their distribution and quality control.

This paper identifies a methylome-dependent restriction barrier in emm1 group A streptococci that limits transformation and presents an improved protocol using phage anti-restriction protein Ocr to overcome this barrier.

The paper identifies synthetic-lethal interactions of Bam complex associated genes in E. coli, revealing dependencies on lipid composition and peptidoglycan synthesis.

This study identifies Aeg1 as a new essential cell division protein in Acinetobacter baumannii that recruits FtsN to initiate cell division.

The paper shows that in Myxococcus xanthus, CdbA regulates CdbS levels to maintain chromosome organization, and its depletion leads to CdbS over-accumulation, causing cell death, especially under heat stress.

The paper studies the mar operon in E. coli using single-cell lineage tracking, revealing inducible memory of promoter activity across generations.

This paper identifies ornithine as the central intermediate in the arginine and citrulline degradation pathway in Bacillus subtilis and shows it activates the transcription regulator RocR.

This study characterizes Type 4 pili-mediated natural competence in Fusobacterium nucleatum, showing DNA uptake and chromosomal integration require specific pilus components and DNA methylation.

The paper investigates the role of periplasmic helices and cytoplasmic domains of PulL and PulM in the assembly of the Klebsiella type II secretion system, showing their critical involvement in protein secretion and pseudopilus formation.

This study demonstrates how bacterial two-component systems can be used to enable transmembrane signaling in synthetic membranes, showing that performance can be tuned by altering membrane properties and protein engineering.

The paper identifies an antisense RNA-mediated regulatory link between mscL and arfA genes in E. coli, modulating MscL excretory activity during osmotic and translational stress.

This study integrates omics data to reveal post-transcriptional regulation mechanisms in Halobacterium salinarum NRC-1, showing 54% of genes are regulated by multiple mechanisms and 7% show conditional regulation.

The paper identifies three distinct DedA subfamilies in bacteria with different phylogenetic distributions and potential functions.

This paper develops a constitutively active type III secretion system in Salmonella to secrete heterologous proteins without inducer reagents, achieving production titers comparable to inducer-based systems.

The paper quantifies direct and indirect effects of global TFs on gene expression in E. coli using deletion mutants and computational analysis.

The paper improves inducible promoters in gram-negative bacteria by enhancing control, strength, and portability for better protein expression.

This paper describes two genetic modules in Lactiplantibacillus plantarum that enable high-level gene expression without antibiotics, bacteriocins, or genomic manipulations.

The paper shows VirB modulates DNA supercoiling to relieve H-NS-mediated silencing in Shigella, independent of transcription or H-NS presence.

This study reveals how the {sigma}B factor induces RNAP oligomerization into a supramolecular complex, leading to auto-inhibition of transcription initiation in Mycobacterium tuberculosis.

This study identifies LexA-regulated proteins in Anabaena under Cd stress, revealing its broader role in stress tolerance beyond photosynthetic redox regulation.

The paper reports cryo-EM structures of functional Rho pre-termination complexes in Escherichia coli, explaining their mechanism and resolving decades of research.

The paper identifies a conserved binding site on bacterial replicative helicases that is targeted by both DciA and DnaC loaders, showing functional interchangeability between these loaders.