Reproductive Biology and Fertility

This study uses single-nuclei multi-omics analysis to identify molecular and cellular changes in human ovarian ageing and links genetic variants to age at natural menopause.

The paper uses machine learning to analyze embryo developmental timing and shows that maternally aged embryos lose temporal regulation, impacting viability.

Lhx9 haploinsufficient mice show subfertility, altered steroid gene expression, and abnormal ovarian surface epithelium.

This study shows that depletion of Dis3 in oocytes leads to persistent transcription and fertility loss due to disrupted RNA processing and epigenetic changes.

This study shows that adding antibiotics to mouse embryo culture media alters gene expression related to DNA integrity, potentially compromising genomic stability.

The paper maps temporal and spatial dynamics of follicle development in mice by analyzing single-cell and spatial transcriptomes, revealing distinct stromal cell populations with specific roles in follicle development.

This study uses single-cell RNA-seq to analyze age-related changes in mouse ovarian microenvironment metabolism, identifying HIF1A's role in modulating glycolysis and other metabolic pathways.

This study shows that sperm-oocyte interactions cause global and localized changes in cortical ER cluster structure during fertilization in mice.

The paper shows that mouse oocytes receive mitochondria from germline-soma via transzonal nanotubes, contributing to mtDNA inheritance and oogenesis.

This study identifies a quiescent-like state in arrested IVF embryos and shows that modulating metabolic pathways can rescue the arrest.

The paper develops a deep learning method to quantify pronuclear (PN) features in embryos and links PN area to chromosomal abnormalities using clinical data.

This study identifies ESR2 as a regulator of Indian hedgehog signaling in neonatal rat ovaries, showing its role in controlling primordial follicle activation.

This study integrates single-cell ATAC-seq and RNA-seq to reveal the epigenetic and transcriptional changes in human ovarian aging, identifying key pathways and a predictive model for oocyte aging.

This paper describes a method using autologous mitochondria from UC-MSCs to rejuvenate aged oocytes through granulosa cell-oocyte aggregation and transzonal filopodia-mediated transfer, improving oocyte maturation and developmental potential.

The paper identifies a PGR-A isoform-RUNX interaction that drives chromatin remodelling at promoters during ovulation, revealing a mechanism for ovulation-specific gene regulation.

This study shows that codon composition affects mRNA stability and protein abundance in human oocytes, with age-related changes in these associations impacting oocyte quality.

The paper shows that a small increase in CHEK1 activity causes arrest of the first zygotic division in human embryos, with implications for assisted reproduction.

The paper analyzes transcriptome and methylome data from mouse oocytes to understand aging and maturation, identifying biomarkers and a predictive model for oocyte quality.

The paper uses single-cell RNA sequencing to show cyclophosphamide induces apoptosis in human ovarian primordial follicles, not follicle growth activation, causing acute ovarian reserve depletion.

This study shows the Hira complex is essential for male pronucleus formation in mice and humans, with Cabin1 and Ubn1 playing critical roles, and demonstrates potential for IVF therapy.

The paper examines how different fertilization methods affect preimplantation gene regulation in mouse embryos, identifying stable and flexible gene expression patterns and the role of sperm RNAs in development.

This study shows that DUX4 regulates the oocyte-to-embryo transition in humans by activating enhancers and promoting genome reprogramming.

The paper establishes that ZP1 mutations cause zona pellucida absence and female infertility in both rats and humans by sequestering ZP3 and ZP4 intracellularly.

This study shows that the DNA damage response, specifically CHK2, regulates oocyte elimination and follicle formation in mammals by controlling meiotic recombination and cyst breakdown.

The paper uses single-cell RNA sequencing to analyze gene expression dynamics in mouse ovarian germ and granulosa cells during primordial follicle assembly, identifying gene clusters, trajectories, and regulatory networks.

This study shows that human oocytes with DNA damage can complete meiosis-I and extrude the first polar body, unlike mouse oocytes which arrest due to SAC signalling.

This study shows that NAD+ repletion with NMN improves oocyte quality and fertility in aged animals, and enhances IVF success in older mice.

This study shows that retinoic acid receptors are not required for meiosis initiation in mouse fetal ovaries, challenging the current model and suggesting a role for CYP26B1 in meiosis prevention.

The paper shows that inhibiting L1 reverse transcriptase and the Chk2 checkpoint prevents fetal oocyte attrition, preserving the ovarian reserve.