Bacterial biofilms and quorum sensing

Nitric oxide tunes secreted metabolite bioactivity

This study shows nitric oxide modifies secreted metabolites' activity, reducing their antibiotic effects and causing toxicity to the producing bacteria.

pH-dependent antibacterial activity of N-acetylcysteine against Staphylococcus aureus and metabolic alterations induced at neutral pH

This study shows N-acetylcysteine's antibacterial activity against S. aureus depends on pH, with neutral pH reducing activity and altering metabolism without inhibiting growth.

Detecting transcriptional responses and comparing the virulence of Pseudomonas aeruginosa cystic fibrosis isolates in a mung bean model

The paper uses a mung bean model to compare virulence of Pseudomonas aeruginosa CF isolates and analyze transcriptional changes during infection.

Contact-dependent communication shapes the mesoscale spatial organization of bacterial swarms

This paper develops mathematical methods to analyze bacterial swarm structures and finds that cell-cell communication and kin recognition shape population architecture during collective migration.

Increased Low Molecular Weight Mucins in Muco-Obstructive Airway Disease Limit Staphylococcus aureus Growth

This study shows low molecular weight mucins in muco-obstructive airway diseases suppress Staphylococcus aureus growth and biofilm formation, while high molecular weight mucins do not, suggesting species-specific interactions.

A versatile dual-color bacterial reporter system highlights two distinct Pseudomonas aeruginosa Type 3 secretion system intracellular populations.

The paper introduces a dual-color bacterial reporter system, pCG-VmS, for tracking gene and protein expression in Pseudomonas aeruginosa, revealing two distinct T3SS populations.

Macrophages reshape cytokine responses and bacterial spatial organization in an airway epithelial cell culture model

This study shows that macrophages alter cytokine responses and bacterial spatial organization in airway epithelial cultures without changing bacterial burden.

In situ three-dimensional mapping of oxygen gradients in Staphylococcus epidermidis biofilms using a solution-based, ratiometric imaging platform

This paper maps oxygen gradients in Staphylococcus epidermidis biofilms using a ratiometric imaging platform and shows how vancomycin treatment affects oxygen levels and biofilm metabolism.

Identification of genes important for response of Pseudomonas aeruginosa biofilms to ciprofloxacin exposure

This study identifies genes critical for Pseudomonas aeruginosa biofilm survival under ciprofloxacin exposure, revealing key pathways and potential therapeutic targets.

Quorum-Sensing Stimulation and Phytochemical Quenching Reshape Biofilm-Associated Gene Expression in Salmonella enterica

This study shows that external quorum-sensing stimulation and phytochemicals alter biofilm-associated gene expression in Salmonella, with strain-specific responses.

An N, S-acetylated L-cysteine-cysteamine conjugate hinders pyocyanin redox cycling to weaken Pseudomonas aeruginosa biofilm and dampens LPS-driven acute pulmonary inflammation

This paper identifies I-152 as a compound that disrupts Pseudomonas aeruginosa biofilm formation and reduces LPS-induced inflammation through redox cycling inhibition and anti-inflammatory effects.

Growth in confinement promotes Pseudomonas aeruginosa tolerance to antibiotics

This study shows that Pseudomonas aeruginosa becomes more tolerant to antibiotics when grown in confined spaces, due to mechanical stress-induced changes in efflux and membrane remodeling.

Motile and matrix-producing cells drive distinct modes of cell-scale motion in Bacillus subtilis colonies

This study shows that motile and matrix-producing cells in Bacillus subtilis colonies drive distinct modes of cell-scale motion, with matrix-producing cells causing slow, ballistic movement.

Flow-driven delivery boosts antibiotic effectiveness by overwhelming bacterial defenses

This study shows flow enhances antibiotic effectiveness by overcoming bacterial defenses in Pseudomonas aeruginosa through microfluidic experiments and simulations.

Detoxification of host-relevant H2O2 shapes Pseudomonas aeruginosa population gradients in flow

This study shows how Pseudomonas aeruginosa uses local H2O2 detoxification to protect downstream cells in flow, creating spatial gradients.

SdiA as a Repressor of Phagocytosis and Intracellular Survival in Klebsiella pneumoniae: Insights from Macrophage and Amoeba Models

This paper shows that SdiA in Klebsiella pneumoniae represses phagocytosis and intracellular survival, with mutant strains being more susceptible to phagocytosis but showing increased intracellular fitness and host cell death.

The conserved coordination of acyl-homoserine lactone and PqsE signaling defines the RhlR-dependent quorum-sensing network in Pseudomonas aeruginosa clinical isolates

This study shows that both C4HSL and PqsE are essential for RhlR-dependent gene expression in Pseudomonas aeruginosa clinical isolates, revealing a conserved regulatory module for virulence.

Biofilm dispersion in Enterococcus faecalis is mediated by nutrient step-change and intra-species signaling

This study identifies nutrient step-change and intra-species signaling as triggers for biofilm dispersion in E. faecalis, reducing antibiotic resistance in dispersed cells.

Therapeutic Relevance of NLPA Lipoprotein to Combat Biofilm-Associated infection in Acinetobacter baumannii

The paper identifies two compounds targeting NLPA, a lipoprotein involved in biofilm formation in Acinetobacter baumannii, showing antibacterial and biofilm inhibition activity in vitro and reduced intracellular survival in macrophages.

Evolutionary Trajectories of Ciprofloxacin Resistance in P. aeruginosa Lung Biofilms: Mutation Dynamics, Metabolomic Shifts, and Collateral Sensitivity

This study tracks the evolution of ciprofloxacin resistance in P. aeruginosa biofilms, revealing metabolic shifts and collateral sensitivity to other antibiotics.

Cooperative antibiotic response in coupled biofilm and planktonic E. faecalis communities

The paper studies how resistant and sensitive E. faecalis bacteria interact under antibiotic stress, showing cooperative behavior that allows survival at high drug concentrations.

Mycobacterium abscessus promotes Pseudomonas aeruginosa biofilm formation and antibiotic tolerance

This study shows that Mycobacterium abscessus enhances Pseudomonas aeruginosa biofilm formation and antibiotic tolerance in co-culture, potentially contributing to treatment challenges in cystic fibrosis patients.

Susceptibility of Klebsiella pneumoniae Clinical Isolates in Biofilms to Antibiotics and Assessment of Secondary Drug Effects

This study evaluates antibiotic susceptibility of K. pneumoniae biofilms and identifies colistin and tigecycline as most effective against biofilm biomass and metabolic activity.

Label-free microscopy enables high-throughput identification of genes controlling biofilm development

This study introduces LFAB, a label-free microscopy method for high-throughput biofilm analysis, and identifies key genes regulating biofilm formation in Streptococcus pneumoniae.

A network perspective on the role of c-di-GMP-associated protein complexes in biofilm formation.

This study identifies a network of c-di-GMP-binding proteins in Pseudomonas fluorescens SBW25, revealing DipA as a central hub that integrates opposing modules for biofilm formation and environmental adaptation.

Phenotypic Analysis of GGDEF/EAL Domain Protein Function in Phytopathogenic Pantoea ananatis

This study identifies and characterizes 26 genes involved in c-di-GMP metabolism in Pantoea ananatis, revealing 14 key proteins that regulate phenotypes like motility and biofilm formation.

Identification of DksA as a novel pro-inflammatory mediator of Pseudomonas aeruginosa under conditions mimicking chronic cystic fibrosis lung infection

A three-component logic gate governs quorum sensing-regulated killing of Stenotrophomonas maltophilia by Pseudomonas aeruginosa

This study identifies a three-component logic gate involving HQNO, PqsE, and PQS biosynthesis that governs Pseudomonas aeruginosa-mediated killing of Stenotrophomonas maltophilia.

A transcription factor-sRNA-mediated double-negative feedback loop confers pathogen-specific control of quorum-sensing genes

The paper identifies a double-negative feedback loop between LuxT and VqmR in V. cholerae that regulates quorum-sensing genes and biofilm formation.

PhoPQ is an upstream regulator of quorum sensing in small colony variant subpopulations of Pseudomonas aeruginosa

This study identifies PhoPQ as a regulator of quorum sensing in small colony variants of Pseudomonas aeruginosa, showing its role in virulence factor production and subpopulation adaptation.