Bacterial biofilms and quorum sensing

Multilevel computational approach to unlock the potential inhibitors of biofilm-EPS, persistence and quinolone signalling in Pseudomonas aeruginosa using mangrove-derived bioactive phytochemicals

This study identifies potential inhibitors of Pseudomonas aeruginosa biofilm-EPS, persistence, and quinolone signalling using mangrove-derived phytochemicals through in-silico methods.

Experimental and theoretical approaches reveal that antimicrobial blue light killing efficiency decreases with biofilm growth in a Pseudomonas aeruginosa model.

Pushing for survival: Spatial intermixing and indirect resistance enable collective growth

The paper shows how spatial organization and mechanical pushing enable susceptible bacteria to survive antibiotic stress through collective behavior.

Molecular basis of quorum-sensing signal transduction by CqsS and its inhibition by CqsA the autoinducer synthase

This study determines the cryo-EM structures of CqsS and its interaction with CqsA and CAI-1, revealing the molecular mechanism of quorum-sensing signal transduction in Vibrio harveyi.

Pseudomonas aeruginosa metabolite 3-oxo-C12HSL induces apoptosis through T2R14 and the mitochondrial calcium uniporter

This study shows that 3-oxo-C12HSL from Pseudomonas aeruginosa induces apoptosis in HNSCC cells via T2R14 and mitochondrial calcium uniporter.

Domain-swapped LuxR-type quorum sensing receptors reveal divergent ligand-response mechanisms among homologs

This paper investigates how structural features of LuxR-type receptors determine their ligand-response mechanisms, showing that the ligand-binding domain primarily dictates associative vs. dissociative activity.

Partitioning the roots of interactions between microbes (PRISM): environment-supplied resources versus species-produced mediators

The paper investigates how microbial interactions are shaped by environmental resources versus species-produced metabolites using a controlled assay with nasal bacterial strains.

Cell motility enhances metabolic coupling in spatially structured microbial communities

This study shows that cell motility in microbial communities disrupts clonal clustering, increases spatial intermixing, and enhances metabolic interactions and community productivity.

A branching cell-fate decision in biofilm dispersal enables long-term surface persistence

The paper shows that during biofilm dispersal, a subpopulation of cells forms a resilient post-dispersal biofilm community that can re-populate the niche and resist antibiotics and bacteriophages.

Enterococcus faecalis alters antibiotic susceptibility in Pseudomonas aeruginosa mixed species biofilms.

This study shows that Enterococcus faecalis increases antibiotic resistance in Pseudomonas aeruginosa within iron-limited biofilms by inducing specific transcriptional changes.

Interspecies synergism and antagonism induce differential and potentially exploitable susceptibility to various classes of antibiotics in a wound-like polymicrobial community

This study shows that polymicrobial communities can alter antibiotic susceptibilities through synergism and antagonism, and demonstrates that combining gentamicin and cephalexin can effectively clear such infections.

Charged agar surfaces affect E. coli biofilm properties by balancing curli amyloid quantity and quality

This study shows that charged agar surfaces alter E. coli biofilm properties by influencing curli amyloid quantity and quality, affecting structure and stability.

Rapid activation of dormant type IV pili enables a dispersal-infection tradeoff in environments with fluctuating nutrients

This study shows Pseudomonas aeruginosa uses energy-dependent T4P idling to balance dispersal and infection in fluctuating nutrient environments.

New inhibitors of the Pseudomonas aeruginosa enzyme, PqsE, and methods assessing their potential to induce a conformational change via active site binding

This study designs and evaluates new inhibitors of PqsE, an enzyme in P. aeruginosa, to disrupt its interaction with RhlR and reduce virulence.

Pseudomonas aeruginosa deploys competitor-specific antagonistic strategies

This study shows Pseudomonas aeruginosa uses different antagonistic strategies to outcompete Burkholderia cenocepacia and Staphylococcus aureus, depending on the competitor's growth rate.

Transcriptional profiling of Pseudomonas aeruginosa biofilm life cycle stages reveals dispersal-specific biomarkers

This study identifies dispersal-specific transcriptional biomarkers in Pseudomonas aeruginosa biofilms by analyzing gene expression across attachment, maturation, and dispersal stages.

A novel Flavobacterium quisquiliarum porphyrin binding protein independently disrupts Pseudomonas aeruginosa biofilms

The paper identifies a porphyrin-binding protein from Flavobacterium quisquiliarum that disrupts Pseudomonas aeruginosa biofilms by sequestering porphyrins and affecting iron homeostasis.

A Triple-Modality Peptide-Antibiotic-Phage Therapy Eradicates Multidrug-Resistant Serratia marcescens Biofilms

This study evaluates a triple-modality therapy combining bacteriophages, antibiotics, and antimicrobial peptides to eradicate multidrug-resistant S. marcescens biofilms in vitro.

Metal-independent effects of calprotectin on cocultures of Pseudomonas aeruginosa and Staphylococcus aureus involve alkylquinolone production

This study shows that calprotectin affects Pseudomonas aeruginosa and Staphylococcus aureus cocultures through metal-independent mechanisms involving alkylquinolone production and quorum sensing.

RhlR quorum-sensing receptor ligand sensitivity regulates the differential expression of phenazine genes in Pseudomonas aeruginosa

This study identifies key residues in RhlR that determine ligand specificity and shows how ligand-driven gene regulation affects phenazine gene expression in Pseudomonas aeruginosa.

Osmotic pressure gradients in E. coli biofilms revealed by in-situ sensors

The paper develops liposomal nano-sensors to measure osmotic pressure gradients in E. coli biofilms, revealing radial increases linked to biofilm structure, metabolism, and expansion.

Extracellular matrix chemistry tunes bacterial biofilm metabolism and optimizes fitness

The paper shows how matrix chemistry in bacterial biofilms affects metabolism and fitness by modulating pyocyanin retention and electron transfer efficiency.

Pseudomonas aeruginosa balances cytotoxicity and motility to counter phagocytosis by macrophages

This study shows that Pseudomonas aeruginosa uses motility to enhance phagocytosis by macrophages, while reduced motility in chronic infections helps evade engulfment, and T3SS activity enables acute immune evasion.

Pseudomonas aeruginosa lasR Mutants Resist Phagocytosis and Alter Inflammatory Cytokine Production by Cystic Fibrosis Macrophages

Aggregation-mediated microcolony shields bacteria from contact-dependent competition and maintains phenotypic heterogeneity

This study shows that type 1 fimbriae and adhesins promote microcolony formation, shielding bacteria from short-range contact-dependent weapons and maintaining phenotypic heterogeneity.

A toolbox of FRET-based c-di-GMP biosensors and its FRET-To-Sort application for genome-wide mapping of the second messenger regulatory network

The paper develops FRET-based c-di-GMP biosensors and applies them with FRET-To-Sort to map regulatory networks controlling c-di-GMP levels in E. coli.

Azotobacter vinelandii AmrZ is a global regulator linking alginate production and c-di-GMP homeostasis

This paper identifies AmrZ as a global regulator in Azotobacter vinelandii that links alginate production to c-di-GMP homeostasis by directly activating algD and specific DGCs, while reducing motility.

The phosphodiesterase NbdA links c-di-GMP signaling to type IV pili function in Pseudomonas aeruginosa PAO1

This paper shows that NbdA, a phosphodiesterase in Pseudomonas aeruginosa, modulates c-di-GMP levels and affects type IV pilus function, influencing surface behavior and phage resistance.

T6SS mutants exploit itaconate to support infection of phagocytes

This study shows that Pseudomonas aeruginosa mutants lacking the H3-T6SS exploit itaconate to persist in phagocytes, enhancing their ability to cause chronic lung infections.

The Chromobacterium Volatilome is Strongly Influenced by Growth on Liquid versus Solid Media

This study compares the volatilomes of Chromobacterium species grown on liquid and solid media, finding that media type has the largest impact on volatile organic compound profiles.