Research on Leishmaniasis Studies

Molecular Detection and Parasite Load Determination of Leishmania donovani in Dogs at Humera and Sheraro, Western Tigray, Ethiopia

The paper was withdrawn due to lack of full author consent, and the authors request it not be cited as a reference.

LeishMANIAdb: a comparative resource for Leishmania proteins

LeishMANIAdb compiles experimental and predicted Leishmania secretomes and host-pathogen interactions to aid in understanding virulence mechanisms and identifying therapeutic targets.

The role of aneuploidy and polyclonality in the adaptation of the Protozoan parasite Leishmania to high drug pressure

This study investigates how aneuploidy and polyclonality contribute to Leishmania's adaptation to high drug pressure, showing different dynamics depending on the drug and pre-existing mutations.

Genomic Analysis of Two Phlebotomine Sand Fly Vectors of Leishmania from the New and Old World

The paper sequences and annotates the genomes of two sand fly species, identifying genes related to vector biology and population structure to aid in leishmaniasis control.

Four layer multi-omics reveals molecular responses to aneuploidy in Leishmania

This study uses multi-omics analysis to show that aneuploidy in Leishmania causes proportional changes in transcript and protein abundance, with post-transcriptional regulation compensating for some effects.

Leishmania major centrin knock-out parasites alter the Kynurenine- aryl hydrocarbon receptor signaling to produce a pro-inflammatory response

This study shows that Leishmania major centrin knock-out parasites alter host tryptophan metabolism to reduce kynurenine-AhR signaling and induce a pro-inflammatory response.

Novel CRISPR-based detection of Leishmania species

This paper develops CRISPR-based assays for detecting Leishmania species, showing high sensitivity and specificity for genus and subgenus level identification.

Spergulin-A strives intra-macrophage leishmanicidal activity by regulating the host P2X7R-P38MAPK axis

This paper shows Spergulin-A, a plant compound, kills Leishmania donovani inside macrophages by activating the P2X7R-P38MAPK signaling pathway.

Polyamines shift expression of macrophage L-arginine metabolism related-genes during Leishmania amazonensis infection

The paper shows polyamines alter gene expression related to L-arginine metabolism in macrophages infected with Leishmania amazonensis, affecting parasite survival and immune response.

Functional study of Leishmania braziliensis protein arginine methyltransferases (PRMTs) reveals that PRMT1 and PRMT5 are required for macrophage infection

This study functionally characterizes PRMTs in Leishmania braziliensis, showing PRMT1 and PRMT5 are required for macrophage infection and axenic amastigote proliferation.

Transcriptional shift and metabolic adaptations during Leishmania quiescence using stationary-phase and drug pressure as models

The paper quantifies transcriptome and metabolome changes in Leishmania during quiescence induced by drug pressure or stationary phase, revealing global transcriptional reduction and specific upregulation of membrane and autophagy-related genes.

High genome plasticity and frequent genetic exchange in Leishmania tropica isolates from Afghanistan, Iran and Syria

The paper analyzes genomic variation in Leishmania tropica isolates to reveal mechanisms of genetic exchange and genome plasticity associated with treatment failure and relapse.

Genome instability drives epistatic adaptation in the human pathogen Leishmania

The paper shows genome instability in Leishmania enables fitness gain through epistatic gene copy number variations and compensatory post-transcriptional mechanisms.

High throughput single-cell genome sequencing gives insights into the generation andevolution of mosaic aneuploidy in Leishmania donovani

The paper uses high throughput single-cell genome sequencing to study mosaic aneuploidy in Leishmania donovani, finding rapid karyotype changes and chromosome-specific adaptations.

From the origin and molecular diversity of the amastins, to the origin and diversity of intracellular parasitism from human Trypanosomatids

This paper investigates the molecular diversity and functional roles of amastins in human Trypanosomatids, showing their involvement in intracellular parasitism and immune evasion through glycosylation, phosphorylation, and interactions with host molecules.

Exploring Leishmania-Host Interaction with Reactome, a Database of Biological Pathways and Processes

The paper curates a Leishmania infection pathway in Reactome to enhance pathway analysis of transcriptomic data from macrophage infections, revealing new insights into IL10 and IL6 modulation.

Variable bites and dynamic populations; new insights in Leishmania transmission

This study develops a probabilistic model to simulate Leishmania transmission dynamics, showing that skin heterogeneity affects short-lived flies while vector population dynamics dominate for longer-lived flies.

Molecular detection of Leishmania donovani, Leishmania major, and Trypanosoma spp. in Sergentomyia squamipleuris sandflies from a visceral leishmaniasis focus in Merti sub-County, eastern Kenya

The paper detects Leishmania and Trypanosoma species in sandflies from Kenya, implicating Sergentomyia squamipleuris in leishmaniasis transmission and suggesting zoonotic potential.

Testing the CRISPR-Cas9 and glmS ribozyme systems in Leishmania tarentolae

The paper tests CRISPR-Cas9 and glmS ribozyme systems for gene disruption and knock-down in Leishmania tarentolae, finding success with CRISPR-Cas9 and limited effectiveness with glmS.

Distinct cis-acting elements govern purine-responsive regulation of the Leishmania donovani nucleoside transporters, LdNT1 and LdNT2

The paper identifies distinct cis-acting elements regulating purine-responsive gene expression in Leishmania donovani, specifically for LdNT1 and LdNT2.

Discovery of tREP-18, a novel class of tRNA encoded peptide with potent leishmanicidal activity

The paper reports a novel tRNA-derived peptide, tREP-18, with potent anti-leishmanial activity against L. donovani and PKDL isolates.

Ecological divergence and hybridization of Neotropical Leishmania parasites

This study examines the evolutionary history and hybridization of Leishmania parasites using whole genome sequencing, revealing ecological divergence and genetic mosaic ancestry in hybrids.

The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samples

The paper shows Leishmania donovani lacks detectable DNA methylation, enabling enrichment of parasite DNA from mixed samples using methyl-binding columns.

Characterization of a novel amastin-like surface protein (ALSP) of Leishmania donovani, a probable lipase

This study characterizes a novel Leishmania donovani protein, ALSP, identifies it as a lipase, and suggests its role in virulence and potential as a vaccine target and biomarker.

Phlebotomus papatasi sand fly salivary protein diversity and immune response potential in Egypt and Jordan populations

The paper analyzes genetic variability and immune response potential of salivary proteins in Phlebotomus papatasi sand flies from Egypt and Jordan.

Leish-ExP: a database of exclusive proteins from Leishmania parasite

The paper introduces Leish-ExP, a database of Leishmania exclusive proteins, identified through whole genome comparative analysis across five species.

Purine-responsive expression of the Leishmania donovani NT3 purine nucleobase transporter is mediated by a conserved RNA stem-loop

The paper identifies a conserved RNA stem-loop in the LdNT3 mRNA 3' UTR that mediates purine-responsive upregulation in Leishmania donovani, with species-specific functionality due to variant bases.

Genomic analysis of natural intra-specific hybrids among Ethiopian isolates of Leishmania donovani

The paper analyzes whole-genome sequences of Leishmania donovani isolates from Ethiopia to investigate hybridization patterns and recombination in natural populations.

Global genome diversity of the Leishmania donovani complex

The paper presents a comprehensive genome-wide study of Leishmania donovani complex isolates, revealing genetic diversity and structural variation across species and geographical regions.

Exploring the evolution and adaptive role of mosaic aneuploidy in a clonal Leishmania donovani population using high throughput single-cell genome sequencing

This paper uses high-throughput single-cell genome sequencing to analyze karyotype variation in a clonal Leishmania donovani population, revealing mosaic aneuploidy and its potential adaptive role.