Metalloenzymes and iron-sulfur proteins

Component A2 is a redox-sensitive archaeal ATPase activated by methyl-coenzyme M reductase

This paper identifies component A2 as an ATPase essential for reductive activation of methyl-coenzyme M reductase (MCR) under anaerobic conditions.

Enhanced production of nitrogenase components in Nicotiana benthamiana through co-expression with Bacterioferritin A

This paper shows that co-expressing Bacterioferritin A with NifU in Nicotiana benthamiana increases NifU and NifH production, aiding nitrogenase cofactor assembly.

Electron confurcation drives photosynthetic H2 production in cyanobacteria

This study identifies the electron partners of the HoxEFUYH hydrogenase in cyanobacteria, revealing its role in coupling reduced ferredoxin with NADH for H2 production.

Azotobacter vinelandii glutaredoxin D delivers the core cluster to nitrogenase cofactor scaffold protein NifU

This study shows that GrxD delivers [Fe2S2] clusters to NifU, enabling [Fe4S4] synthesis and nitrogenase activity in Azotobacter vinelandii.

An Unprecedented Cluster in a Methanotroph Acetol Dehydrogenase

The paper discovers and characterizes a novel [Cu-4Fe-4S] cluster in acetol dehydrogenase from Methylacidiphilum fumariolicum SolV, revealing its unique structure and high redox potential.

A connection between two ancient and essential cellular processes, iron-sulfur protein biogenesis and fatty acid synthesis, in Escherichia coli

This study identifies a molecular link between iron-sulfur cluster biogenesis and fatty acid synthesis in E. coli through the interaction of ACP and IscS.

Phylogenetic based dissection of eukaryotic Mo-insertase functionality: From mechanism to complex assembly

The paper investigates the evolutionary and functional diversity of eukaryotic Mo-insertases using phylogenetic analysis, sequence comparison, and structural modeling, revealing domain organization plasticity and strict active site conservation.

Evolution-guided engineering of an ancient nitrogenase interface enhances enzyme activity and stability

The paper identifies a tunable interface in nitrogenase that can be engineered to enhance enzyme activity and stability through targeted mutations.

Nitrogenase regulation in Vibrio natriegens differs from other γ-proteobacteria

This study identifies the regulatory mechanisms of nitrogenase in Vibrio natriegens, showing that NtrBC is not essential but important for timely expression, while NifA autoactivation suffices for nitrogenase gene expression.

Nitrosomes: protein language modeling and live-cell imaging reveal condensate-like nitrogenase organization in heterocysts

The paper uses live-cell imaging and a homology-aware framework to identify nitrogenase organization in heterocysts and prioritize cyanobacterial condensate candidates.

Correspondence on "Fortification of FeS Clusters Reshapes Anaerobic CO Dehydrogenase into an Air-Viable Enzyme ThroughMultilayered Sealing of O2 Tunnels"

The paper challenges claims about oxygen resistance in CO dehydrogenase mutants by showing A559W and A559W/V610H variants are not more resistant than wild-type.

A conserved isoleucine gates the diffusion of small ligands to the active site of NiFe CO-dehydrogenase

The paper investigates the role of conserved isoleucine 563 in controlling ligand access to the active site of NiFe CO-dehydrogenase through site-directed mutagenesis.

Evidence for an Organometallic Species Formed in the ArsL Reaction

The paper investigates how ArsL suppresses 5'-dA* formation and discovers an organometallic species involving ACP and the [Fe4S4] cluster to facilitate carbon-arsenic bond formation.

Horizontal gene transfer drives the emergence of nitrogen fixation in a unicellular Synechocystis lineage

This study identifies a functional nitrogen fixation system in Synechocystis sp. LKSZ1 acquired via horizontal gene transfer from filamentous cyanobacteria.

Coupling of electron-bifurcation modules powers aromatic ring reduction beyond the biological redox window

This study reveals how BCRII enzymes use electron-bifurcation modules to reduce aromatic rings beyond typical biological redox limits.

Hierarchical decoding of targeting tripeptide motif by the cytosolic iron-sulfur cluster assembly targeting complex

This paper defines the molecular basis for TCR peptide recognition by the CIA targeting complex using binding measurements, bioinformatics, and structural modeling, revealing a hierarchy of physicochemical determinants.

Investigating the native functions of -carbon monoxide dehydrogenase through genomic context analysis

The paper uses genomic context analysis to investigate the functional roles of different CODH clades, revealing distinct gene clustering patterns and regulatory associations.

Self-Sufficient Maturation and Catalysis of a Clade E CODH Encoded in a CooCTJ-Operon from Clostridium pasteurianum BC1

This study characterizes a clade E CODH from Clostridium pasteurianum BC1, showing moderate CO oxidation and CO2 reduction activities, and identifies its maturation machinery's role in stability.

Nar1 binds the cytosolic iron sulfur cluster assembly targeting complex via a bipartite interaction interface

This study identifies the molecular mechanism by which Nar1 binds the CTC through two distinct interfaces, facilitating Fe-S cluster transfer during protein maturation.

Predicting compatibility between ferredoxins and the Fe protein of nitrogenase using in silico protein modeling

This study predicts ferredoxin compatibility with nitrogenase Fe protein using in silico docking, finding that shorter cofactor distances enhance electron tunneling and compatibility.

Tuning the H2 Production Activity of ToHydA by Molecular Simulation-Informed Protein Engineering

This study uses molecular simulations and mutagenesis to investigate the role of an additional cysteine in enhancing H2 production activity of ToHydA without affecting O2 stability.

Moraxella catarrhalis HemW is a Heme-binding Radical SAM Enzyme

This study characterizes McHemW, a heme-binding radical SAM enzyme in Moraxella catarrhalis, showing it binds heme and contains an active [4Fe-4S] cluster, suggesting a role in heme transport.

Use of Substrate Analogues and X-ray spectroscopy Reveals an all Ferrous C-Cluster in CO Dehydrogenase

This paper uses X-ray spectroscopy and substrate analogs to determine the redox states and electronic structure of the C-cluster in Ni-Fe CODH, revealing an all-ferrous cluster in its catalytically active states.

Carbon monoxide-driven proton respiration enables facultative anaerobes to survive electron acceptor limitation

This paper shows that facultative anaerobes use CO-dependent proton respiration to survive electron acceptor limitation, mediated by Ni-CODH/ECH, which is redox-regulated and provides a reliable energy source in variable environments.

A New Paradigm of Transcriptional Regulation by the SufR-Like Iron-Sulfur Transcription Factors

This study reveals the structural and functional mechanism of SufR-like iron-sulfur transcription factors in bacteria, showing a novel DNA recognition strategy and expanding their known evolutionary distribution.

Molecular mechanism of nitrogenase sequestration by a PII-protein couple

This paper elucidates how anaerobes regulate nitrogenase activity through the PII-protein couple NifI1 and NifI2, revealing a structural mechanism for energy-dependent N2-fixation control.

Atmospheric hydrogen consumption is regulated by catabolite repression in mycobacteria

The paper shows that catabolite repression regulates hydrogen consumption in Mycobacterium smegmatis by modulating the expression of the huc operon in response to glycerol availability.

FeSBCP Analogue from Cyanobacteria: Insights from in vitro and in silico Studies

The paper characterizes a cyanobacterial FeSBCP using bioinformatics, biochemistry, and biophysics, showing efficient binding and repair of 6-4 photolesions.

Rieske Iron-Sulfur Cluster Proteins from an Anaerobic Ammonium Oxidizer Suggest Unusual Energetics in their Parent Rieske/cytochrome b complexes

The paper presents crystal structures and electrochemical data on Rieske subunits from anammox bacteria, supporting a novel NAD(P)H generation mechanism with unusual redox potentials and structural features.

Methylthio-alkane reductases use nitrogenase metalloclusters for carbon-sulfur bond cleavage

This study presents the first molecular structure of methylthio-alkane reductase, revealing metalloclusters that inform its function and evolutionary relationship to nitrogenases.