Metalloenzymes and iron-sulfur proteins

Proteomic changes orchestrate metabolic acclimation of a unicellular diazotrophic cyanobacterium during light-dark cycle and nitrogen fixation states

This study identifies proteomic changes in Crocosphaera subtropica during light-dark cycles and nitrogen fixation, revealing coordinated regulation of metabolic pathways and nitrogenase expression.

Structural analysis of the NifL-NifA complex reveals the molecular basis of anti-activation of nitrogen fixation gene expression in Azotobacter vinelandii

The paper uses cryo-EM to study the NifL-NifA complex in Azotobacter vinelandii, revealing how NifL sequesters NifA to regulate nitrogen fixation and enabling engineered strains with enhanced nitrogen secretion.

Temporal regulation of metabolic processes in the marine diazotroph Crocosphaera watsonii WH 8501

This study identifies temporal regulation of metabolic processes in Crocosphaera watsonii WH8501 using proteomics, revealing coordinated protein abundance patterns across diel cycles.

Feedback regulation of iron-sulfur cluster biogenesis

The paper identifies a feedback mechanism in Mycobacterium tuberculosis where [2Fe-2S] clusters inhibit sulfide supply by regulating the SufS/SufU complex.

Two conserved arginine residues facilitate C-S bond cleavage and persulfide transfer in Suf family cysteine desulfurases.

This study investigates the role of two conserved arginine residues in SufS, showing they are critical for persulfide transfer during iron-sulfur cluster biogenesis in E. coli.

Structure of active methyl-CoM reductase, Earth's main methane producer

The paper determines the structure of active Ni(I)-Methyl-CoM reductase, revealing how it catalyzes methane formation and providing insights for methane emission control and biofuel production.

Cryo-EM captures the coordination of long-range allostery and asymmetric electron transfer by a bi-copper cluster in the nitrogenase-like DPOR complex

The paper uses Cryo-EM to reveal how asymmetry and allostery coordinate long-range electron transfer in the DPOR enzyme through a bi-copper cluster.

Protein dynamics affect O2-stability of Group B -hydrogenase from Thermosediminibacter oceani

This study identifies ToHydA, an O2-stable [FeFe]-hydrogenase from Thermosediminibacter oceani, and reveals structural features that protect the H-cluster from O2-induced degradation.

Biological molybdenum usage stems back to 3.4 billion years ago

This study uses phylogenomic analysis to show that molybdenum-dependent enzymes existed as early as 3.4 billion years ago, suggesting Mo was bioavailable in ancient oceans.

Characterization of nitrogenase genes nifP and nifVZT1 in the cyanobacterium Anabaena (Trichormus) variabilis ATCC 29413

This study characterizes the nitrogenase genes nifP and nifVZT1 in Anabaena variabilis, showing they are cotranscribed but have distinct promoters and regulatory mechanisms.

The Cia1 and Cia2 subunits of the CTC mediate recognition of apo-FeS proteins with a C-terminal targeting complex recognition motif

This study identifies the Cia1-Cia2 interface as critical for recognizing TCR motifs on client proteins, revealing a broader role for CIAO2A in iron-sulfur protein maturation.

Yeast cluster

This study characterizes the iron-sulfur cluster binding properties of Nar1 using spectroscopic and mass spectrometry methods, revealing multiple cluster binding sites and oxygen sensitivity.

Molecular principles of redox-coupled sodium pumping of the ancient Rnf machinery

This study elucidates the molecular mechanism of redox-coupled sodium pumping in the Rnf complex using cryo-EM, biochemical assays, and simulations.

Investigation of the Cyanothece nitrogenase cluster in Synechocystis: a blueprint for engineering nitrogen fixing photoautotrophs

The paper investigates the nitrogenase gene cluster in Cyanothece to identify essential genes for nitrogen fixation in phototrophs and provides a blueprint for synthetic nitrogen-fixing systems.

Structure-based analysis unveils co-origin of LPOR and nitrogenase-like proteins

The paper identifies structural links between nitrogenase-like proteins, LPOR, and chlorophyllide oxidoreductases, revealing their evolutionary origins and functional evolution through structural analysis.

Molecular sorting of nitrogenase catalytic cofactors

This study identifies AnfO as a protein that prevents misincorporation of incorrect cofactors into Fe-only nitrogenase during its maturation.

HCF101 is a novel component of the CIA cytosolic iron-sulfur synthesis pathway in the human pathogen Toxoplasma gondii

This paper identifies HCF101 as a novel component of the cytosolic Fe-S assembly pathway in Toxoplasma gondii, essential for parasite survival and a potential drug target.

Snapshots of acetyl-CoA synthesis, the final step of CO2 fixation in the Wood-Ljungdahl pathway

The paper presents structural snapshots of the CODH/ACS complex in CO2 fixation, revealing conformational changes and reaction mechanisms for acetyl-CoA synthesis.

Evolutionary plasticity and functional repurposing of the essential metabolic enzyme MoeA

The paper investigates how the MoeA enzyme evolved multifunctionality through evolutionary and structural analyses, showing its repurposing in both bacteria and eukaryotes for diverse roles.

Ecological resource competition as a driver of metallome evolution

The paper explains the evolutionary timing of nitrogenase isozymes by linking ecological competition for molybdenum to changes in environmental conditions after the Great Oxidation Event.

Structural evolution of nitrogenase enzymes over geologic time

The paper reconstructs the structural evolution of nitrogenase enzymes over 3 billion years using phylogenetics, ancestral sequence reconstruction, crystallography, and deep learning.

Protonation/deprotonation-driven switch for the redox stability of low-potential ferredoxin

This study identifies the protonation state of Asp64 as a critical factor controlling the redox stability of the [4Fe-4S] cluster in low-potential ferredoxins.

A nitrogenase-like enzyme is involved in the novel anaerobic assimilation pathway of a sulfonate, isethionate, in the photosynthetic bacterium Rhodobacter capsulatus

This paper identifies a nitrogenase-like enzyme (IsrBHDK) involved in the anaerobic assimilation of isethionate in Rhodobacter capsulatus, revealing a new pathway in the sulfur cycle.

The structure of the SufS-SufE complex reveals interactions driving protected persulfide transfer in iron-sulfur cluster biogenesis

This paper presents the first X-ray structure of the E. coli SufS/SufE complex, revealing interactions critical for persulfide transfer in Fe-S cluster biogenesis.

Combinatorial in silico approach for cancer-associated 4Fe-4S protein discovery

This study identifies potential cancer-associated 4Fe-4S proteins using three computational traits, discovering 21 novel candidates beyond known ones.

Modular analysis of a logical regulatory network reveals how Fe-S cluster biogenesis is controlled in the face of stress

The paper develops a logical model of Fe-S cluster biogenesis in E. coli to analyze how environmental stressors like iron deprivation and oxidative stress regulate this process.

Resolving phylogenetic and biochemical barriers to functional expression of heterologous iron-sulphur cluster enzymes

The paper investigates why iron-sulphur cluster enzymes are often inactive when heterologously expressed in E. coli and identifies strategies to improve their functionality.

A pathogenic role for histone H3 copper reductase activity in a yeast model of Friedreich's Ataxia

The paper shows that reducing histone H3-mediated Cu1+ production restores Fe-S cluster deficiencies in yeast, linking chromatin and mitochondrial function to Fe-S cluster homeostasis.

Transcriptional analysis of nitrogen fixation in Paenibacillus durus during growth in nitrogen-enriched medium

The paper analyzes the transcriptional regulation of nitrogen fixation in Paenibacillus durus under nitrogen-enriched and deficient conditions, finding that nifH transcripts are present but nitrogen fixation activity is absent, suggesting post-transcriptional or post-translational regulation.

Recombination of 2Fe-2S ferredoxins reveals differences in the inheritance of thermostability and midpoint potential

This study uses homologous recombination to create ferredoxin chimeras, revealing how thermostability and midpoint potential are inherited differently.