Metalloenzymes and iron-sulfur proteins

Redox Regulation in O2-Tolerant Hydrogenases: Insights from two homologues.

This study compares two [FeFe] hydrogenases, showing that TgHydA1 remains more stable under oxygen than CbHydA1 due to slower reactivation rates.

Cryo-EM structure of a methanogen nitrogenase-PII protein supercomplex

The paper reports a cryo-EM structure of a nitrogenase-PII supercomplex in methanogens, revealing a metabolite-gated assembly mechanism for nitrogenase inhibition.

Comprehensive study on ferredoxin isoforms in the cyanobacterium Synechocystis sp. PCC 6803

This study identifies and characterizes ferredoxin isoforms in Synechocystis sp. PCC 6803, revealing their redox properties and functional roles in electron transfer under varying environmental conditions.

Molecular basis of N2 fixation in a hyperthermophilic archaeon

This study investigates the N2-fixing system of Methanocaldococcus infernus, revealing structural insights into its thermostable nitrogenase and unifying mechanistic principles across nitrogenase types.

Structural insights into how metallochaperones UreE and UreG interact to deliver a toxic metal to urease

This study determines the crystal structure of the UreE2G2 complex bound with nickel ions and GMPPNP, revealing how GTP and nickel binding regulate nickel transfer between metallochaperones UreE and UreG.

Promiscuous Metal Site in Hepatitis B Virus X Protein Binds anFe-S Cluster

This study identifies Fe-S cluster ligands in HBx, showing it binds both Fe-S and Zn, with implications for HBV replication and drug targeting.

Multiheme selenoenzyme essential for elemental sulfur respiration

This study identifies a multiheme selenoenzyme essential for elemental sulfur respiration in bacteria, revealing its unique catalytic mechanism involving selenium and heme.

De Novo Design of a Protein Binder to Probe Gas Channel and Enhance the Oxygen Tolerance of Hydrogenase

This study designs AI-guided protein binders to map O2 diffusion pathways in hydrogenase, enhancing oxygen tolerance by blocking primary channels.

The anaerobic fungus Caecomyces churrovis produces H2 via a non-bifurcating NADH-dependent enzyme complex

This paper identifies a non-bifurcating NADH-dependent enzyme complex in anaerobic fungi responsible for H2 production, distinct from ferredoxin-dependent pathways.

Partial Bypass of Frataxin Deficiency by ISCU M141I Restores Cytosolic and Nuclear FeS Cluster Assembly

The paper shows that the ISCU M141I variant partially rescues Fe-S cluster assembly in FXN-deficient cells, preserving nuclear and cytosolic Fe-S proteins but not mitochondrial function.

Direct Binding of Cysteine-367 Thiolate to the Active Site of the -Hydrogenase from Clostridium beijerinckii in the O2-stable State

The paper presents a high-resolution crystal structure of CbA5H in the O2-stable Hinact state, showing direct thiol binding to the active site iron, supported by DFT calculations.

Accurate prediction of nitrogen fixation in cyanobacteria reveals the dynamic evolution driving high retention rate with mosaic distribution

This study predicts nitrogen fixation in cyanobacteria using a 2D similarity plot, revealing a mosaic distribution and higher retention rate in the Cyanobacteria phylum.

The structure of the SufBC2D-SufE complex reveals the mechanism of sulfur transfer in bacterial Fe-S cluster assembly.

This study determines the structure of the SufBC2D-SufE complex, revealing conformational changes that facilitate sulfur transfer in bacterial Fe-S cluster assembly.

Component A2 is a redox-sensitive archaeal ATPase activated by methyl-coenzyme M reductase

This paper identifies component A2 as an ATPase essential for reductive activation of methyl-coenzyme M reductase (MCR) under anaerobic conditions.

Azotobacter vinelandii glutaredoxin D delivers the core cluster to nitrogenase cofactor scaffold protein NifU

This study shows that GrxD delivers [Fe2S2] clusters to NifU, enabling [Fe4S4] synthesis and nitrogenase activity in Azotobacter vinelandii.

A connection between two ancient and essential cellular processes, iron-sulfur protein biogenesis and fatty acid synthesis, in Escherichia coli

This study identifies a molecular link between iron-sulfur cluster biogenesis and fatty acid synthesis in E. coli through the interaction of ACP and IscS.

Phylogenetic based dissection of eukaryotic Mo-insertase functionality: From mechanism to complex assembly

The paper investigates the evolutionary and functional diversity of eukaryotic Mo-insertases using phylogenetic analysis, sequence comparison, and structural modeling, revealing domain organization plasticity and strict active site conservation.

Correspondence on "Fortification of FeS Clusters Reshapes Anaerobic CO Dehydrogenase into an Air-Viable Enzyme ThroughMultilayered Sealing of O2 Tunnels"

The paper challenges claims about oxygen resistance in CO dehydrogenase mutants by showing A559W and A559W/V610H variants are not more resistant than wild-type.

Investigating the native functions of -carbon monoxide dehydrogenase through genomic context analysis

The paper uses genomic context analysis to investigate the functional roles of different CODH clades, revealing distinct gene clustering patterns and regulatory associations.

Predicting compatibility between ferredoxins and the Fe protein of nitrogenase using in silico protein modeling

This study predicts ferredoxin compatibility with nitrogenase Fe protein using in silico docking, finding that shorter cofactor distances enhance electron tunneling and compatibility.

Tuning the H2 Production Activity of ToHydA by Molecular Simulation-Informed Protein Engineering

This study uses molecular simulations and mutagenesis to investigate the role of an additional cysteine in enhancing H2 production activity of ToHydA without affecting O2 stability.

Carbon monoxide-driven proton respiration enables facultative anaerobes to survive electron acceptor limitation

This paper shows that facultative anaerobes use CO-dependent proton respiration to survive electron acceptor limitation, mediated by Ni-CODH/ECH, which is redox-regulated and provides a reliable energy source in variable environments.

FeSBCP Analogue from Cyanobacteria: Insights from in vitro and in silico Studies

The paper characterizes a cyanobacterial FeSBCP using bioinformatics, biochemistry, and biophysics, showing efficient binding and repair of 6-4 photolesions.

Rieske Iron-Sulfur Cluster Proteins from an Anaerobic Ammonium Oxidizer Suggest Unusual Energetics in their Parent Rieske/cytochrome b complexes

The paper presents crystal structures and electrochemical data on Rieske subunits from anammox bacteria, supporting a novel NAD(P)H generation mechanism with unusual redox potentials and structural features.

Methylthio-alkane reductases use nitrogenase metalloclusters for carbon-sulfur bond cleavage

This study presents the first molecular structure of methylthio-alkane reductase, revealing metalloclusters that inform its function and evolutionary relationship to nitrogenases.

Proteomic changes orchestrate metabolic acclimation of a unicellular diazotrophic cyanobacterium during light-dark cycle and nitrogen fixation states

This study identifies proteomic changes in Crocosphaera subtropica during light-dark cycles and nitrogen fixation, revealing coordinated regulation of metabolic pathways and nitrogenase expression.

Structural analysis of the NifL-NifA complex reveals the molecular basis of anti-activation of nitrogen fixation gene expression in Azotobacter vinelandii

The paper uses cryo-EM to study the NifL-NifA complex in Azotobacter vinelandii, revealing how NifL sequesters NifA to regulate nitrogen fixation and enabling engineered strains with enhanced nitrogen secretion.

Two conserved arginine residues facilitate C-S bond cleavage and persulfide transfer in Suf family cysteine desulfurases.

This study investigates the role of two conserved arginine residues in SufS, showing they are critical for persulfide transfer during iron-sulfur cluster biogenesis in E. coli.

Cryo-EM captures the coordination of long-range allostery and asymmetric electron transfer by a bi-copper cluster in the nitrogenase-like DPOR complex

The paper uses Cryo-EM to reveal how asymmetry and allostery coordinate long-range electron transfer in the DPOR enzyme through a bi-copper cluster.

Protein dynamics affect O2-stability of Group B -hydrogenase from Thermosediminibacter oceani

This study identifies ToHydA, an O2-stable [FeFe]-hydrogenase from Thermosediminibacter oceani, and reveals structural features that protect the H-cluster from O2-induced degradation.