Biochemical and Structural Characterization

Int&in: a machine learning-based web server for split site identification in inteins

Int&in is a web server that predicts active and inactive split sites in inteins using a Gaussian Naive Bayes model with 0.76 accuracy.

Sortase A-based Post-Translational Modifications on Encapsulin Nanocompartments

This paper develops a sortase A-mediated system in E. coli to site-specifically attach proteins to encapsulin nanocompartments, both internally and externally, without in vitro steps.

Cell surface sculpting using logic-gated protein actuators

This paper introduces a protein actuator platform that performs Boolean logic operations on cell surfaces using proximity-gated trans-splicing, enabling selective recruitment of enzymatic and cytotoxic activities to specific cells.

In silico discovery of the myxosortases that process MYXO-CTERM and three novel prokaryotic C-terminal protein-sorting signals that share invariant Cys residues

This paper identifies four novel C-terminal protein-sorting signals and their cognate processing enzymes in prokaryotes using computational analysis.

Ffp1, an ancestral Porphyromonas spp. fimbrillin

The paper identifies Ffp1 as the sole fimbrillin in Porphyromonas species, showing it is ancestral and structurally similar to Bacteroides ovatus fimbrillin.

Visible light-induced specific protein reaction delineates early stages of cell adhesion

This paper uses visible light to control specific protein reactions in mammalian cells, revealing early stages of cell adhesion and mechanotransduction.

A Multiplexed Bacterial Two-Hybrid for Rapid Characterization of Protein-Protein Interactions and Iterative Protein Design

The paper develops a high-throughput method to characterize protein-protein interactions and design orthogonal coiled-coil pairs, identifying a large set of specific interactions.

Using LanM Enzymes to Modify Glucagon-Like Peptides 1 and 2 in E.coli

The paper uses LanM enzymes and leader peptides to modify D-Ala in GLP-1 and GLP-2 in E. coli, showing how precursor design affects modification patterns.

Identification of functional Npu DnaE and gp41-1 inteins split in three fragments

The paper identifies new split sites in Npu DnaE and gp41-1 inteins, enabling functional three-piece versions for biotechnological applications.

Myxosortases process MYXO-CTERM and other bacterial C-terminal protein-sorting signals that have invariant Cys residues.

The paper identifies myxosortases as proteases processing C-terminal sorting signals with invariant cysteine residues in bacteria.

helixCAM: A Platform for Programmable Cellular Assembly in Bacteria and Human Cells

The paper introduces helixCAM, a platform for engineering specific cell-cell interactions in bacteria and human cells using coiled-coil peptides, enabling multicellular assembly applications.

Augmenting the Signal Peptide of the Ag43 Autotransporter for the improved heterologous display of sfGFP using Fluorescence-Activated Cell Sorting (FACs)-assisted natural selection.

The paper improves the signal peptide of the Ag43 autotransporter in E. coli to enhance surface display of sfGFP using epPCR and single-cell sorting.

Engineering functional membrane-membrane interface by InterSpy

The paper introduces InterSpy, a synthetic biology tool that forms membrane-membrane interfaces using SpyTag/SpyCatcher, and demonstrates its function in cell-free systems and cells.

Large Scale Discovery of Microbial Fibrillar Adhesins and Identification of Novel Members of Adhesive Domain Families

The paper develops a random forest method to identify fibrillar adhesins in bacterial proteomes, discovering over 6,500 candidates and 24 novel adhesive domain families.

SufB intein splicing in Mycobacterium tuberculosis is influenced by two remote conserved N-extein Histidines

The paper studies the role of two conserved histidines in the intein splicing of SufB in Mycobacterium tuberculosis, showing their impact on splicing and cleavage rates.

Extending Janus lectins architecture: characterization and application to protocells

The paper develops a new chimeric lectin, Janus lectin, with enhanced valency and specificity for fucose and galactose, and demonstrates its application in forming protocell-like structures.

A Universal Chemical Method for Rational Design of Protein-based Nanoreactors

The paper describes a chemical method to design proteasome-like nanoreactors using micelle-assisted labeling and bioconjugation.

Expanding the SiMPl plasmid toolbox for use with spectinomycin/streptomycin

The paper expands the SiMPl plasmid toolbox to include spectinomycin/streptomycin resistance by introducing a new plasmid pair and validating a strategy for splitting antibiotic resistance enzymes.

Coiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion

This paper designs stable coiled-coil peptides for cellular regulation and detects SARS-CoV-2 spike protein-mediated cell fusion.

DNA-Encoded Multivalent Display of Protein Tetramers on Phage: Synthesis and In Vivo Aplications

This paper develops a DNA-encoded method to display multivalent proteins on phage and demonstrates its use to study in vivo clearance rates of L-asparaginase.

Intein-assisted bisection mapping systematically splits proteins for Boolean logic and inducibility engineering

This paper introduces IBM, a method that systematically splits proteins into logic gates using inteins, enabling inducible control of protein function.

Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing

This paper introduces a method to insert synthetic peptides into proteins in eukaryotic cells to enable chemical modifications and non-canonical amino acids, validated using GFP, NaV1.5, and P2X2 receptors.

Control of ΦC31 integrase-mediated site-specific recombination by protein trans splicing

The paper describes a split-intein system for regulating site-specific recombination using protein trans-splicing, demonstrating its application as a reversible AND gate.

Modular Thermal Control of Protein Dimerization

This paper introduces thermomers, temperature-controlled protein dimerization domains that enable remote control of intracellular interactions in various biological contexts.

Molecular Insights on exquisitely Selective SrtA inhibitors towards active site loop forming open/close lid conformations in SrtA from Bacillus anthracis

This study investigates how SrtA inhibitors' activity depends on the closed lid conformation of SrtA from Bacillus anthracis through molecular dynamics and docking analyses.

A genetically encoded adhesin toolbox for programming multicellular morphologies and patterns

This paper introduces a genetically encoded adhesion toolbox in E. coli to program multicellular morphologies and patterns through modular cell-cell adhesion.

Tunable Extracellular Self-Assembly of Multi-Protein Conjugates from Bacillus subtilis

This paper demonstrates a method for tunable extracellular self-assembly of multi-protein conjugates using Bacillus subtilis, enhancing enzyme resilience and enabling functional multi-protein complex formation.