R. N. Kirchdoerfer

An alphacoronavirus polymerase structure reveals conserved co-factor functions

This paper determines the structure of an alphacoronavirus polymerase complex and identifies conserved cofactor interactions important for antiviral drug development.

Role of spike in the pathogenic and antigenic behavior of SARS-CoV-2 BA.1Omicron

This study compares the pathogenicity and immune evasion of an Omicron S-bearing virus with natural Omicron, showing vaccine escape via S mutations but severe disease due to factors outside S.

pH-dependent polymorphism of the structure of SARS-CoV-2 nsp7

This study determines the solution structure of SARS-CoV-2 nsp7 at pH 7.0 using NMR spectroscopy and identifies pH-dependent structural changes in the protein.

From Structure to Sequence: Identification of polyclonal antibody families using cryoEM

This paper uses cryoEM and next-generation sequencing to identify monoclonal antibodies from polyclonal sera and validate their antigen-binding properties.

Mass spectrometric based detection of protein nucleotidylation in the RNA polymerase of SARS-CoV-2

This study detects GMP-modified amino acids in SARS-CoV-2 RdRP proteins using mass spectrometry, identifying novel nucleotidylation sites and suggesting potential drug targets.

Inhibition of SARS-CoV-2 polymerase by nucleotide analogs: a single moleculeperspective

This study investigates how nucleotide analogs like Remdesivir affect SARS-CoV-2 polymerase activity using single-molecule techniques, revealing backtracking rather than termination and evasion of ddhCTP.

A Library of Nucleotide Analogues Terminate RNA Synthesis Catalyzed by Polymerases of Coronaviruses Causing SARS and COVID-19

This paper evaluates nucleotide analogues as inhibitors of SARS-CoV and SARS-CoV-2 RdRp, identifying those that terminate RNA synthesis and resist viral exonuclease activity.

Reporter assays for Ebola virus nucleoprotein oligomerization, virion-like particle budding, and minigenome activity reveal the importance of nucleoprotein amino acid position 111

The paper develops reporter assays to study Ebola virus nucleoprotein (NP) function and finds that amino acid position 111 affects oligomerization, virion budding, and transcription/replication.

Mapping polyclonal antibody responses in non-human primates vaccinated with HIV Env trimer subunit vaccines

This study maps polyclonal antibody responses in non-human primates vaccinated with HIV Env trimer subunit vaccines and identifies epitopes associated with neutralization breadth.

Structure of the SARS-CoV NSP12 polymerase bound to NSP7 and NSP8 co-factors

The paper presents the 3.1 Å resolution structure of SARS-CoV NSP12 polymerase bound to NSP7 and NSP8 co-factors using cryo-EM, revealing its architecture and implications for antiviral drug design.

Structure of the Ebola virus nucleoprotein - RNA complex

The paper presents a 3.1 Å cryo-EM structure of the Ebola virus nucleoprotein-RNA helical filament, providing detailed insights into protein-RNA and protein-protein interactions.

Receptor binding and proteolysis do not induce large conformational changes in the SARS-CoV spike

This study uses cryo-EM to show that ACE2 binding and trypsin cleavage of SARS-CoV spike protein do not cause major conformational changes or expose the S2 cleavage site.