D. N. Kotton

Nascent matrix deposition supports alveolar organoid formation from aggregates in synthetic hydrogels

This study develops a Matrigel-free method using synthetic hydrogels to form alveolar organoids, showing they secrete their own extracellular matrix for growth.

Aberrant macrophage activation and maladaptive lung repair promote tuberculosis progression uniquely in the lung

This study identifies aberrant macrophage activation and maladaptive lung repair as key factors promoting tuberculosis progression in the lung using a novel mouse model.

Generation of human alveolar epithelial type I cells from pluripotent stem cells

This study generates human alveolar epithelial type I cells from iPSCs by activating YAP signaling, revealing Hippo-LATS-YAP's role in their differentiation.

Role of spike in the pathogenic and antigenic behavior of SARS-CoV-2 BA.1Omicron

This study compares the pathogenicity and immune evasion of an Omicron S-bearing virus with natural Omicron, showing vaccine escape via S mutations but severe disease due to factors outside S.

Durable alveolar engraftment of PSC-derived lung epithelial cells into immunocompetent mice

This study demonstrates the durable engraftment of PSC-derived lung epithelial cells into immunocompetent mice, showing long-term retention of both progenitor and differentiated cell phenotypes.

Impact of cell culture on the transcriptomic programs of primary and iPSC-derived human alveolar type 2 cells

This study compares the transcriptomes of primary, cultured, and iPSC-derived human alveolar type 2 cells to assess how culture affects their gene expression profiles and maturation states.

The oral drug nitazoxanide restricts SARS-CoV-2 infection and attenuates disease pathogenesis in Syrian hamsters

The paper shows that nitazoxanide inhibits SARS-CoV-2 replication and pathogenesis in human and hamster models, and synergizes with remdesivir against multiple variants.

Modeling of Alpha-1 Antitrypsin Deficiency with Syngeneic Human iPSC-Hepatocytes Reveals Metabolic Dysregulation and Cellular Heterogeneity in PiMZ and PiZZ Hepatocytes

This study uses CRISPR-Cas9 to create syngeneic iPSC-derived hepatocytes with ZAAT heterozygosity, revealing metabolic dysregulation and cellular heterogeneity in MZ and ZZ cells.

Morphological Cell Profiling of SARS-CoV-2 Infection Identifies Drug Repurposing Candidates for COVID-19

This study identifies 17 FDA-approved compounds with antiviral efficacy against SARS-CoV-2 using high-throughput screening and finds lactoferrin as a promising drug candidate with multiple antiviral mechanisms.

Recombinant Lloviu virus as a model to study inaccessible zoonotic viruses

This study uses recombinant Lloviu virus to investigate replication mechanisms and host interactions of an uncultured filovirus related to Ebola.

Human airway lineages derived from pluripotent stem cells reveal the epithelial responses to SARS-CoV-2 infection

This study uses iPSC-derived airway cells to model SARS-CoV-2 infection, showing multiciliated cells as primary targets and demonstrating antiviral responses to remdesivir and camostat methylate.

Patient-specific iPSCs carrying an SFTPC mutation reveal the intrinsic alveolar epithelial dysfunction at the inception of interstitial lung disease

This study uses patient-derived iPSCs with an SFTPC mutation to model early alveolar epithelial dysfunction in interstitial lung disease, revealing proteostasis, metabolic, and inflammatory changes.

SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway

This study identifies SARS-CoV-2 inhibition of the JAK-STAT pathway as a mechanism to suppress interferon signaling across diverse cell types.

SARS-CoV-2 induces double-stranded RNA-mediated innate immune responses in respiratory epithelial derived cells and cardiomyocytes

This study investigates how SARS-CoV-2 interacts with host antiviral pathways in respiratory and cardiac cells, finding it activates OAS-RNase L and PKR but not IFN, contrasting with MERS-CoV.

Amyloidogenic Proteins Drive Hepatic Proteostasis Remodeling in an Induced Pluripotent Stem Cell Model of Systemic Amyloid Disease

This study uses iPSC-derived hepatic cells to show that liver proteostasis factors and UPR signaling correlate with TTR amyloid production and can reduce amyloid secretion through ER stress-independent activation.