E. O. Saphire

Structure of a SARS-CoV-2 spike S2 subunit in a pre-fusion, open conformation

This study presents a pre-fusion, open conformation of the SARS-CoV-2 S2 subunit and identifies antibodies that recognize it, providing insights into spike dynamics and potential vaccine targets.

Intracellular Ebola Virus nucleocapsid assembly revealed by in situ cryo-electron tomography.

This study uses cryo-electron tomography to reveal the structure of the intracellular Ebola virus nucleocapsid, identifying a new outer layer of NP complexed with VP35 and conserved assembly interfaces.

Identification of mouse CD4+ T cell epitopes in SARS-CoV-2 BA.1 spike and nucleocapsid for use in peptide:MHCII tetramers

The paper identifies SARS-CoV-2 BA.1 epitopes recognized by mouse CD4+ T cells and generates corresponding tetramer reagents for studying immune responses.

Lymphocytic choriomeningitis arenavirus utilises tunnelling nanotube-like intercellular connections for cell-to-cell spread

This paper shows LCMV spreads between cells via tunnelling nanotube-like structures, bypassing extracellular transmission.

Cellular endosomal potassium ion flux regulates arenavirus uncoating during virus entry

This study identifies potassium ion flux as a third requirement for LCMV entry, showing it facilitates uncoating by modulating NP/Z interactions.

HLA topography enforces shared and convergent immunodominant B cell and antibody alloresponses in transplant recipients

The paper identifies an immunodominant B cell response targeting specific HLA residues in transplant recipients, suggesting antigenic topography drives antibody-mediated rejection.

Spatial and functional arrangement of Ebola virus polymerase inside phase-separated viral factories

This study investigates the spatial and functional organization of Ebola virus polymerase within viral factories, revealing distinct structural forms and their roles in viral replication.

Structure of the Inmazeb cocktail and resistance to escape against Ebola virus

The paper presents the cryoEM structure of the Inmazeb cocktail in complex with Ebola virus glycoprotein, showing how the combination prevents escape mutations better than monotherapy.

Proximity interactome analysis of Lassa polymerase reveals eRF3a/GSPT1 as a druggable target for host directed antivirals.

The paper identifies eRF3a/GSPT1 as a druggable host target for antiviral therapy against Lassa virus by analyzing its interactome using proximity proteomics.

Structural basis for antibody-mediated neutralization of Lymphocytic choriomeningitis virus

This study presents the cryoEM structure of LCMV surface glycoprotein and shows that the antibody M28 neutralizes the virus by stabilizing its prefusion state, offering a therapeutic candidate for LCMV infection.

Glycan shield of the ebolavirus envelope glycoprotein GP

This study profiles the site-specific glycosylation patterns of ebolavirus GP using mass spectrometry, revealing complex N-, O-, and C-linked glycans that contribute to the glycan shield and restrict antibody neutralization.

Systematic analysis of human antibody response to ebolavirus glycoprotein reveals high prevalence of neutralizing public clonotypes

The paper analyzes the human antibody response to ebolavirus glycoprotein, identifying public clonotypes with neutralizing activity and implications for vaccine design.

Structure of the rabies virus glycoprotein trimer bound to a pre-fusion specific neutralizing antibody

The paper reports the cryo-EM structure of pre-fusion rabies virus glycoprotein bound to the neutralizing antibody RVA122, revealing its trimeric interface and stabilization mechanism.

Functional interactomes of the Ebola virus polymerase identified by proximity proteomics in the context of viral replication

The paper identifies functional interactors of the Ebola virus polymerase using proximity proteomics and validates their role in promoting viral replication.

Collaboration between the Fab and Fc contribute to maximal protection against SARS-CoV-2 in nonhuman primates following NVX-CoV2373 subunit vaccine with Matrix-M™ vaccination

This study shows that NVX-CoV2373 vaccine induces antibodies with both neutralizing and Fc-effector functions, which together provide maximal protection against SARS-CoV-2 in non-human primates.

Immunological memory to SARS-CoV-2 assessed for up to eight months after infection

The paper assesses SARS-CoV-2 immune memory components over eight months post-infection, finding varying kinetics in antibodies, B cells, and T cells.

Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers

The paper determines the structure of VP40 matrix layers in Ebola and Marburg virus-like particles, revealing they are locally ordered assemblies of VP40 dimers forming 2D lattices.

Reporter assays for Ebola virus nucleoprotein oligomerization, virion-like particle budding, and minigenome activity reveal the importance of nucleoprotein amino acid position 111

The paper develops reporter assays to study Ebola virus nucleoprotein (NP) function and finds that amino acid position 111 affects oligomerization, virion budding, and transcription/replication.

Structure of the Ebola virus nucleoprotein - RNA complex

The paper presents a 3.1 Å cryo-EM structure of the Ebola virus nucleoprotein-RNA helical filament, providing detailed insights into protein-RNA and protein-protein interactions.

Structural Basis of Broad Ebolavirus Neutralization by a Human Survivor Antibody

The paper determines the structure of a broadly neutralizing antibody against ebolavirus, identifies a conserved epitope, and explains factors affecting its activity against related viruses.