T. R. Golub

Translation of non-canonical open reading frames as a cancer cell survival mechanism in childhood medulloblastoma

The paper investigates non-canonical ORF translation in medulloblastoma, identifying ASNSD1-uORF as critical for cell survival through interactions with the prefoldin-like chaperone complex.

Partial gene suppression improves identification of cancer vulnerabilities when CRISPR-Cas9 knockout is pan-lethal

This study compares CRISPR-Cas9 and RNAi screens to identify cancer vulnerabilities, finding that RNAi better detects pan-dependencies while CRISPR identifies more dependencies overall.

CODEC enables 'single duplex' sequencing

CODEC improves mutation detection by combining NGS throughput with single-molecule resolution, achieving similar accuracy with fewer reads.

Phosphate dysregulation via the XPR1:KIDINS220 protein complex is a therapeutic vulnerability in ovarian cancer

The paper identifies XPR1 and KIDINS220 as therapeutic targets in ovarian cancer by showing their role in phosphate dysregulation leading to cell death.

Selective vulnerability of aneuploid human cancer cells to inhibition of the spindle assembly checkpoint

This study identifies aneuploid cancer cells as more vulnerable to spindle assembly checkpoint (SAC) inhibition and explores the underlying mechanisms.

Non-canonical open reading frames encode functional proteins essential for cancer cell survival

The paper identifies non-canonical open reading frames (ORFs) that encode functional proteins essential for cancer cell survival, with one example, GREP1, showing preferential essentiality in breast cancer.

Multiplexed single-cell profiling of post-perturbation transcriptional responses to define cancer vulnerabilities and therapeutic mechanism of action

The paper introduces MIX-Seq, a method for multiplexed single-cell transcriptional profiling of cancer cell responses to perturbations, revealing drug mechanisms and predicting cell viability.

Non-oncology drugs are a source of previously unappreciated anti-cancer activity

The paper identifies non-oncology drugs with anti-cancer activity across 578 cancer cell lines using PRISM, finding that many drugs selectively inhibit cancer cells based on molecular features.

Agreement between two large pan-cancer CRISPR-Cas9 gene dependency datasets

This study compares two large pan-cancer CRISPR-Cas9 screens, finding high concordance in gene dependencies despite differences in protocols and reagents.

Patient-derived xenografts undergo murine-specific tumor evolution

This study shows that PDX tumors acquire new genomic changes over passages, differ from human tumors, and affect drug responses.