Adam J. Mead

CAR T cell therapy selectively depletes disease-driving mutant calreticulin cells in xenotransplants and human organoid models of myelofibrosis

A first-in-class CAR T-cell therapy targeting mutant calreticulin selectively depletes malignant stem cells in vivo and in fibrotic human organoids.

Redefining the topology of the human bone marrow using augmented spatial transcriptomic analysis

This study uses spatial transcriptomic analysis and machine learning to redefine the topology of human bone marrow in health and myeloproliferative neoplasms, revealing new insights into cellular heterogeneity and fibrosis.

Genome editing for treatment of JAK2 V617F-driven myeloproliferative neoplasms

This study uses CRISPR-Cas genome editing to selectively inactivate JAK2 V617F mutations in patient cells, showing that heterozygous cells are not oncogene addicted while homozygous cells are eradicated, suggesting genome editing as a potential treatment for MPNs.

Hypercholesterolaemia promotes epigenetic memory in hematopoietic stem cells that persists after lipid lowering and causes systemic immunometabolic dysfunction mediated by macrophage metabolic reprogramming

This study shows that high cholesterol induces epigenetic changes in hematopoietic stem cells that persist after cholesterol reduction, leading to systemic immunometabolic dysfunction.

comBO: A combined human bone and lympho-myeloid bone marrow organoid for pre-clinical modelling of haematopoietic disorders

This paper introduces comBOs, a new human bone and lympho-myeloid bone marrow organoid model that includes multiple cell types and improves upon existing models for studying haematopoietic disorders.

Robust CRISPR/dCas13 RNA blockers specifically perturb miRNA-target interactions and rescue type 1 myotonic dystrophy pathology

This paper develops CRISPR-Lock, a CRISPR/dCas13 RNA blocker that disrupts miRNA-target interactions and rescues DM1 pathology by correcting splicing biomarkers and reducing nuclear foci.

MARVEL: An integrated alternative splicing analysis platform for single-cell RNA sequencing data

MARVEL is an R package for single-cell alternative splicing analysis, benchmarked against existing tools and applied to iPSC differentiation studies.

Developmental stage- and site-specific transitions in lineage specification and gene regulatory networks in human hematopoietic stem and progenitor cells

This study identifies site- and stage-specific transitions in gene regulatory networks and lineage specification in human hematopoietic stem and progenitor cells across developmental stages.

Unraveling intratumoral heterogeneity through high-sensitivity single-cell mutational analysis and parallel RNA-sequencing

This study introduces TARGET-seq, a method that simultaneously detects mutations and performs RNA-sequencing in single cells, revealing transcriptional and genetic tumor heterogeneity in myeloproliferative neoplasm cells.

From pioneer to repressor: Bimodal foxd3 activity dynamically remodels neural crest regulatory landscape in vivo

This study shows foxd3 acts as a pioneer factor and then switches to a repressor in neural crest cells, dynamically remodeling chromatin structure to control stemness and cell fate.

Telomerecat: A Ploidy-Agnostic Method For Estimating Telomere Length From Whole Genome Sequencing Data

Telomerecat estimates telomere length from whole-genome sequencing data without requiring prior knowledge of ploidy, showing good performance and detecting expected patterns in various data types.