Henning Stahlberg

Cryo-ET and Sub-Volume Analysis of Fibrillar Pathology in the Neurodegenerative Human Brain

This paper uses cryo-ET to study amyloid fibrils in neurodegenerative brain tissue, revealing ultrastructural details of fibril polymorphs and their association with disease.

Ultrastructural remodelling of tau fibrils during ghost tangle formation in Alzheimer's disease brain

This study uses correlative light and electron microscopy to characterize the ultrastructural changes in tau fibrils during ghost tangle formation in Alzheimer's disease brain tissue.

Correlative Ultrastructural Mapping of Lewy Pathology Reveals Regional Diversity in Parkinsons and Dementia with Lewy bodies

This study uses correlative light and electron microscopy to analyze Lewy body pathology in Parkinson's and dementia with Lewy bodies, revealing regional differences in ultrastructure and mitochondrial changes.

Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules

This study investigates whether pulsed electron beams reduce radiation damage in cryo-EM and finds no significant difference compared to conventional illumination.

Temperature-dependent ligand relocation reveals plasticity of TRPM4 inhibition

This study shows that the inhibitor PBA binds to TRPM4 in different locations at 8°C and 37°C, revealing temperature-dependent changes in ligand recognition.

Ultrastructural diversity and subcellular organization of nigral Lewy pathology in Parkinson's disease

The paper examines ultrastructural differences between somatic and neuritic Lewy body inclusions in Parkinson's disease using correlative light and electron microscopy.

Structural basis for a filamentous morpheein model of human cystathionine beta-synthase

This study reveals the structural basis of CBS as a filamentous morpheein, showing how dynamic quaternary transitions regulate its activity and stability.

CryoWriter: A Robotic Solution for Improved Cryo-EM Grid Preparation

The paper introduces a robotic system for cryo-EM grid preparation that improves sample efficiency and reconstruction quality.

Deconvolving the structural heterogeneity of alpha-Synuclein in vitro and in situ

This paper develops a method to quantitatively analyze the structural states of alpha-synuclein in vitro and in situ, revealing conformational changes in yeast and human neurons.

Cryo-EM structure of alpha-Synuclein Fibrils Harboring the Dementia with Lewy Bodies - Associated E83Q Mutation

The paper determines the cryo-EM structure of alpha-synuclein fibrils with the E83Q mutation, revealing structural changes linked to enhanced aggregation and pathogenicity.

Synthetic alpha-synuclein fibrils replicate in mice causing MSA-like pathology

This study shows synthetic alpha-synuclein fibrils can replicate in mice, causing MSA-like pathology and forming glial cytoplasmic inclusions.

Arrestin recognizes GPCRs independently of the receptor state

This study shows arrestin2 binds to the β1-adrenergic receptor independently of receptor conformation or ligand type, driven by a conformational change in arrestin induced by a phosphopeptide.

Multiscale analysis of myelin alterations in skin biopsies from synucleinopathies.

The paper analyzes myelin structure and protein abundance in skin biopsies from synucleinopathy patients to identify structural differences and potential biomarkers.

Correlative light and electron microscopy for human brain and other biological models

This paper provides a protocol for correlative light and electron microscopy (CLEM) optimized for analyzing human brain tissue, enabling detailed ultrastructural analysis and characterization of subcellular features.

Structural basis of SIRT7 nucleosome engagement and substrate specificity

This study determines the structural basis of SIRT7's specificity for H3K36 and H3K18 deacylation by solving nucleosome-bound SIRT7 structures and analyzing enzyme-nucleosome interactions.

Mouse α-synuclein fibrils are structurally and functionally distinct from human fibrils associated with Lewy body diseases

This study reports the atomic structure of mouse α-synuclein fibrils and shows they differ structurally and functionally from human fibrils linked to Parkinson's and MSA.

Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution

This paper applies low-dose cryo-EM and 4D-STEM ptychography to achieve sub-nanometer resolution 3D reconstructions of frozen hydrated proteins.

Rapid iPSC inclusionopathy models shed light on formation, consequence and molecular subtype of alpha-synuclein inclusions

This paper develops an iPSC platform to rapidly generate alpha-synuclein inclusions and investigates their formation, subtypes, and molecular interactions.

Structure-function analysis of the cyclic beta-1,2-glucan synthase

The paper determines the structure of cyclic beta-1,2-glucan synthase from Agrobacterium tumefaciens and clarifies its mechanism of action.

Cryo-EM structure of alpha-synuclein fibrils amplified by PMCA from PD and MSA patient brains

Three fibril polymorphs, amplified from Parkinson's and multiple system atrophy brain homogenate by PMCA, adopt folds unlike any published alpha-synuclein arrangement; their disease relevance is untested.

High-resolution cryo-EM structure of urease from the pathogen Yersinia enterocolitica

The paper reports the high-resolution cryo-EM structure of urease from Yersinia enterocolitica, revealing the bimetal active site and enabling drug development insights.

Two new polymorphic structures of alpha-synuclein solved by cryo-electron microscopy

The paper reports two new polymorphic structures of alpha-synuclein fibrils solved by cryo-EM, showing distinct structural features and interactions compared to previous structures.

Lewy pathology in Parkinson's disease consists of a crowded organellar, membranous medley

The paper uses high-resolution imaging and biophysical methods to show that Lewy pathology in Parkinson's disease consists mainly of a crowded membranous medley of vesicles and dysmorphic organelles, with only a small fraction containing proteinaceous filaments.

Cryo-EM structure of alpha-synuclein fibrils

The paper reports the cryo-EM structure of alpha-synuclein fibrils, revealing key features relevant to disease mechanisms and therapeutic design.

MRCZ - A proposed fast compressed MRC file format and direct detector normalization strategies

The paper proposes a compressed MRC file format and normalization strategies for direct electron detectors to improve data storage and reduce noise in transmission electron microscopy.