Jeffrey N. Bruce

Expanding the therapeutic window of bacteria for cancer therapy

This paper develops a tumor-targeting engineered bacterium that improves therapeutic efficacy and safety in cancer treatment.

Paired Tumor Biopsies Reveal Spatiotemporal Myeloid Remodeling After Local Chemotherapy in Glioblastoma

This study examines the spatiotemporal myeloid response to chemotherapy in glioblastoma using multiple models, showing chemotherapy-induced genotoxic stress and inflammatory remodeling in myeloid cells.

Electrophysiologically Targeted Biopsies Reveal the Transcriptional Landscape of Focal Epilepsy

This study uses electrophysiologically guided biopsies and single-nucleus RNA sequencing to identify transcriptional differences between seizure focus and ictal penumbra in focal epilepsy.

IDH-mutant inhibitors enhance the sensitivity of IDH1-mutant gliomas to cysteine-methionine deprivation and ferroptosis

This study shows that IDH1-mutant glioma cells are more sensitive to cysteine-methionine deprivation and ferroptosis inducers, and that IDH inhibitors enhance this sensitivity.

Convection-enhanced delivery of dexamethasone in glioma suppresses myeloid inflammation while avoiding systemic toxicities

This paper evaluates convection-enhanced delivery of dexamethasone in glioma models, showing reduced systemic toxicity and suppressed myeloid inflammation.

A proteogenomic tool uncovers protein markers for human microglial states

The paper introduces a proteomic tool to identify protein markers for human microglial states and validates their utility in purification and differentiation.

A therapeutic role for a regulatory glucose transporter1 (Glut1)-associated natural antisense transcript

This paper identifies a natural antisense transcript that regulates Glut1 expression and demonstrates its therapeutic potential in Glut1 deficiency syndrome.

Glioma-Induced Alterations in Excitatory Neurons are Reversed by mTOR Inhibition

This study investigates how mTOR inhibition reverses glioma-induced changes in excitatory neurons, showing effects on mRNA, dendritic spines, and neuronal activity.

Scalable co-sequencing of RNA and DNA from individual nuclei

This paper introduces DEFND-seq, a method for jointly profiling DNA and RNA from individual nuclei, enabling analysis of gene expression with copy number and SNV associations.

A pharmacological toolkit for human microglia identifies Topoisomerase I inhibitors as immunomodulators for Alzheimer's disease

The paper identifies Topoisomerase I inhibitors as immunomodulators for Alzheimer's disease by characterizing their effects on human microglial subtypes in vitro and in a zebrafish model.

Consensus scHPF Identifies Cell Type-Specific Drug Responses in Glioma by Integrating Large-Scale scRNA-seq

The paper introduces consensus scHPF, a method to identify robust gene signatures from scRNA-seq data, and applies it to discover cell type-specific drug responses in glioma.

Pre-clinical validation of an RNA-based precision oncology platform for patient-therapy alignment in a diverse set of human malignancies resistant to standard treatments

The paper validates an RNA-based platform for predicting drug sensitivity in treatment-resistant cancers using patient-derived xenograft models and multi-protein therapies.

Integrating single-cell RNA-seq and imaging with SCOPE-seq2

SCOPE-seq2 integrates single-cell imaging and RNA-seq with improved throughput and accuracy for analyzing human glioblastoma cells.

Single-cell based elucidation of molecularly-distinct glioblastoma states and drug sensitivity

The paper identifies four molecularly distinct glioblastoma subtypes using single-cell protein-activity analysis and shows drug sensitivity is state-dependent.

De novo Gene Signature Identification from Single-Cell RNA-Seq with Hierarchical Poisson Factorization

This paper introduces scHPF, a Bayesian method for discovering gene signatures from scRNA-seq without prior normalization, and finds spatially biased expression patterns in glioma regions linked to poor survival.