wet-lab / biochemical assay

This study shows the circadian clock coordinates Notch and Wnt signaling in muscle stem cells to regulate regeneration, with functional effects in dystrophic muscle and human myoblasts.

This study shows that truncating mutations in PPM1D shift its degradation pathway from ubiquitin-independent to UBR5-mediated, leading to increased PPM1D levels and oncogenic effects.

This study identifies a NOD2-estrogen regulatory axis in zebrafish that impacts intestinal homeostasis, showing estrogen can replicate NOD2-deficiency effects and be reversed by tamoxifen.

This study identifies WRN as a therapeutic target in ARID1A-mutated cancers, showing that its inhibition causes DNA damage and cell death, with potential for combination therapy with p21 inhibition.

TLR5 deficiency is linked to pulmonary fibrosis due to its role in preventing microbial dysbiosis following lung injury.

This study shows that Robo4 deletion increases neuroinflammation and impairs motor coordination in Alzheimer's disease mice, with effects dependent on AD mutations and brain region.

This study shows that GSDMD limits host resistance to Toxoplasma gondii while NINJ1 promotes protection, revealing opposing roles of pyroptotic effectors in IL-1β-dependent immunity.

This paper develops a circular DNAzyme platform (CANDe) that targets the lncRNA JINR1 to suppress Japanese Encephalitis Virus infection.

This paper introduces PRO-MOSAIX, a high-accuracy, ultrasensitive multiplex immunoassay that enables 15-plex detection in human plasma with low femtomolar sensitivity.

This study examines the role of helix O alanine residues in CLC channels, showing that mutations at these sites cause proteostatic defects and impair channel function.

This paper shows that the mycobacterial ligand ManLAM uses structural features to engage TLR2 and Dectin-2, reprogramming macrophage lipid metabolism and promoting foam cell formation.

This study shows SHIP2 exists as a homodimer in cells, and its oligomerization affects interactome and cellular response to sodium arsenate-induced stress granule dynamics.

This study identifies FGFR1-phosphate sensing and GSDMD-mediated NETs as drivers of vascular calcification in CKD, offering therapeutic targets.

This study identifies TRIF and IL-1R1 as regulators of intestinal IgA production, showing their roles in controlling IgA class-switching and germinal center B cell differentiation.

This study identifies MpABCG1 and MpABCG36 as key proteins involved in the accumulation of specialized metabolites in oil bodies of Marchantia polymorpha.

This study introduces RAID-MaP to measure RNA structure within biomolecular condensates, revealing insights into rRNA folding and structural dynamics in the nucleolus and 7SK RNA.

This paper develops ORION, a genetically encoded BRET pH sensor with improved performance and broader pH range, and demonstrates its use in identifying acid imprinting states in cancer cells.

This study identifies PKCδ agonism as a method to selectively induce pyroptosis in NF1-deficient cells by targeting KRAS-GDP interactions.

This paper uses iPSC-derived erythroid cells to model genetic diversity in sickle cell disease and shows they reflect heterogeneous responses to HbF-inducing therapies like hydroxyurea.

This paper characterizes EecP, a novel cell wall-anchored phosphodiesterase in E. faecalis that degrades extracellular c-di-AMP, showing its role in modulating host immune responses and infection outcomes.

The paper identifies lysosomal aspartylglucosaminidase (AGA) as a rate-limiting regulator linking glycoprotein catabolism to glycoRNA formation.

The paper compares acrylamide and butynamide stereoprobes to identify covalent inhibitors of the actin maturation protease ACTMAP in human cancer cells.

This study shows that bacterial brain infection in Drosophila activates Toll and IMD immune pathways in the brain, with glia as the primary responders, and that these responses persist even after bacterial clearance.

This paper identifies soluble CD155 as a systemic checkpoint that suppresses DNAM-1 signaling, leading to resistance to immune checkpoint inhibitors, and shows that an anti-sCD155 antibody can reprogram this signaling to enhance antitumor immunity.

This study shows that reducing Flotillin-2 increases T-DM1 internalization and cytotoxicity in HER2-amplified cancers by enhancing HER2 ubiquitination and internalization.

The paper shows that increasing translation initiation rates accelerates nucleation of aggregating proteins at polysomes, suggesting cotranslational nucleation influences proteome evolution.

This study identifies SIRT2 as a regulator of translation by deacetylating and stabilizing 4EBP1, which suppresses protein synthesis under amino acid limitation.

MDNA is a software module that generates and analyzes complex DNA structures, supporting modifications and providing geometric analysis tools for detailed DNA modeling.

This study identifies PI(3)P binding to the 53BP1 tBRCT domain as essential for promoting 53BP1 condensate-like assembly at DNA double-strand breaks.

This study shows Mrc1 balances replication fork stability and flexibility by linking CMG helicase and Pol2, and its phosphorylation alters this balance under checkpoint activation.