Antibiotic Resistance in Bacteria

Transfer potential of F-like plasmids in Escherichia coli differs by animal environment

The paper examines F-like plasmid transfer potential in E. coli from poultry, bovine, and swine environments, finding higher functional completeness in poultry-associated plasmids.

Genomic analysis of extended-spectrum beta-lactamase-producing Enterobacter kobei ST691 strain harboring mcr-10.1 isolated in Yaounde, Cameroon.

The paper reports the first detection of a colistin-resistant ESBL-producing E. kobei strain isolated from a child in Cameroon, highlighting antimicrobial resistance concerns.

Benefits and costs of a hypercapsule and the mechanism of its loss in a clinical isolate of Acinetobacter baumannii

This study identifies a mutation-driven mechanism for capsule switching in Acinetobacter baumannii, revealing a trade-off between immune evasion and environmental adaptation.

From ecology to evolution: plasmid- and colicin-mediated persistence of antibiotic resistant Escherichia coli in gulls

This study tracks the long-term persistence of antibiotic-resistant E. coli in Caspian gulls, revealing plasmid-encoded traits and competitive dynamics that contribute to resistance maintenance in wildlife.

Resistance, heteroresistance and fitness costs drive colistin treatment failure during Acinetobacter baumannii murine pneumonia

Best of Both Worlds? Optimising Graph-Based Antimicrobial Resistance Gene Profiling in Long and Short-Read Metagenomes

This study evaluates graph-based tools for detecting antimicrobial resistance genes in metagenomes, finding that graph alignment improves detection while preserving genomic context.

Rutin derived microbiota metabolite 3,4 dihydroxybenzoic acid restores antibiotic susceptibility in XDR Gram negative pathogens with a system wide susceptibility signature

This study identifies 3,4-dihydroxybenzoic acid (DHBA), a rutin metabolite, as a potent antibiotic potentiator that restores susceptibility in XDR Gram-negative pathogens through a system-wide genetic susceptibility signature.

Genome-Level Hierarchical Attention Transformer with Multi-Head Attention Weighted Sum for Broad-Spectrum Antimicrobial Resistance Prediction and Discovery of Resistance-Related Genomic Contexts

This paper introduces GL-HAT, a transformer model for predicting antimicrobial resistance and identifying resistance-related genomic contexts, achieving high accuracy and interpretability.

The Quantification of Drug Accumulation within Gram-Negative Bacteria

This paper presents a method to quantify intrabacterial drug accumulation and metabolism in Gram-negative bacteria using liquid chromatography-mass spectrometry, showing correlations with drug efficacy and synergy.

Comprehensive profiling of antibiotic resistance, virulence genes, and mobile genetic elements in the gut microbiome of Tibetan antelopes

This study profiles antibiotic resistance, virulence genes, and mobile genetic elements in Tibetan antelope gut microbiomes, identifying species-specific resistance and potential human pathogen strains.

Nutrient-limited conditions reveal the activity of a minor groove binder, MGB-BP-3, against Escherichia coli

This study shows that MGB-BP-3, a minor groove binder, is more effective against Gram-negative bacteria like E. coli under nutrient-limited conditions than in rich media, with in vivo efficacy in a Galleria mellonella model.

Adaptive Laboratory Evolution under gradual ciprofloxacin stress leads to efflux-mediated ciprofloxacin resistance and an increase in endogenous antibiotic production in Streptomyces coelicolor.

This study uses adaptive laboratory evolution to show that Streptomyces coelicolor develops ciprofloxacin resistance via efflux pumps and increased antibiotic production, with cross-resistance and collateral sensitivity to other drugs.

Performance of the BD Phoenix CPO detect assay for the detection and classification of OXA-48 producing-Escherichia coli that do not coproduce ESBL/pAmpC

The paper evaluates the BD Phoenix CPO Detect assay for detecting OXA-48-producing E. coli without co-producing ESBL/pAmpC, showing high accuracy and rapid results.

A Mechanism Based Pharmacokinetic/Pharmacodynamic Analysis of Polymyxin B-Based Combination Therapy Against Carbapenem-Resistant Klebsiella pneumoniae Isolates with Diverse Phenotypic and Genotypic Resistance Mechanisms

This study develops a mechanism-based pharmacokinetic/pharmacodynamic model to evaluate PMB-based combination therapy against CRKP, showing synergistic effects with lower PMB doses.

Molecular epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae: Risk factors and resistance mechanism of ceftazidime/avibactam in China

This study analyzes carbapenem-resistant hypervirulent Klebsiella pneumoniae strains in China, identifying resistance mechanisms and risk factors for ceftazidime/avibactam.

Strain-dependent contribution of the AcrAB-TolC efflux pump to Klebsiella pneumoniae physiology

This study shows that the AcrAB-TolC efflux pump has strain-specific roles in drug resistance, biofilm formation, and virulence in Klebsiella pneumoniae.

IS1216 drives the evolution of pRUM-like multidrug resistance plasmids in Enterococcus faecium

This study identifies IS1216 as a key driver in the evolution of pRUM-like multidrug resistance plasmids in Enterococcus faecium through comparative genomic analysis of 152 plasmid sequences.

Increasing the predictive accuracy of the Resistance Gene Identifier by evaluating antimicrobial resistance gene over- and underprediction

This study evaluates the accuracy of antimicrobial resistance gene prediction by analyzing bit-score cutoff values in CARD models and identifies sources of over- and underprediction in the RGI tool.

Pangenome structure and ecological adaptation in the Klebsiella pneumoniae species complex: insights from a geographically and time limited multi-habitat study

This study analyzes the pangenome structure and ecological adaptation of Klebsiella pneumoniae species complex across different habitats, revealing phylogroup- and niche-specific genomic features.

Antibiotic-adjuvants abolish resistance conferred by the Staphylococcus aureus erythromycin resistance methyltransferase in an Escherichia coli model

This study identifies an adjuvant, JNAL-016, that inhibits SaErmC-mediated resistance in E. coli, restoring antibiotic efficacy against macrolides and lincosamides.

Intracellular Acinetobacter baumannii Acts as a Reservoir in Lung Infection via a 'Persist and Resist' Strategy

This study shows that intracellular Acinetobacter baumannii can act as a reservoir for recurrent lung infections by evading immune responses and antibiotics through a 'persist and resist' strategy.

The acquisition of rmpADC can increase virulence of classical Klebsiella pneumoniae in the absence of other hypervirulence-associated genes

This study investigates whether the rmpADC operon alone can increase virulence in classical Klebsiella pneumoniae strains, finding that HMV is necessary but not sufficient for hypervirulence.

Structural basis of undecaprenyl phosphate glycosylation leading to polymyxin resistance in Gram-negative bacteria

This study presents cryo-EM structures of ArnC, revealing its conformational changes and catalytic mechanism for aminoarabinose glycosylation in polymyxin resistance.

Evolutionary trajectories determine the feasibility of collateral sensitivity based antibiotic treatment strategies in critical bacterial pathogens

This study investigates collateral sensitivity in bacterial pathogens, finding that resistance mutations can sometimes increase susceptibility to other antibiotics, but these mutations are often costly and not present in clinical isolates.

MetaPointFinder: Detection of Antimicrobial Resistance-Conferring Point Mutations (ARMs) from Metagenomic Reads

MetaPointFinder detects antimicrobial resistance-conferring point mutations from metagenomic reads, outperforming existing methods in simulated data.

Allele-Specific Effects of Mutations in the Rifampin Resistance-Determining Region (RRDR) of RpoB on Physiology and Antibiotic Resistance in Enterococcus faecium

This study examines allele-specific effects of Rifampin Resistance-Determining Region (RRDR) mutations in RpoB on E. faecium physiology and antibiotic resistance, revealing varied impacts on growth, susceptibility, and hospital survival.

Cross-scale effect of microbiota dynamics on resistance

This paper develops a cross-scale model to study how gut microbiota dynamics influence antimicrobial resistance persistence and transmission across hospital and community settings.

Genomic characterization of type I-E* CRISPR-Cas systems in Klebsiella pneumoniae: association of spacer diversity and antimicrobial resistance

This study examines CRISPR-Cas systems in Klebsiella pneumoniae, finding no significant association between CRISPR-Cas and multidrug resistance, but noting diverse spacers in non-MDR strains targeting ARG-bearing plasmids.

Identification of Evolutionary Trade-Offs Associated with High-Level Colistin Resistance in Acinetobacter baumannii

This study identifies evolutionary trade-offs in Acinetobacter baumannii associated with high-level colistin resistance through experimental evolution and genomic analysis.

Binding mode of isoxazolyl penicillins to a Class-A beta-lactamase at ambient conditions

The paper determines the binding modes of three isoxazolyl penicillins to a class-A beta-lactamase at near physiological temperatures using crystallography.