Lipid Membrane Structure and Behavior

This paper introduces a correlative light and electron microscopy workflow to quantify lipid densities in membrane nanodomains and finds sphingomyelin partitions into specific endosomal structures.

This study determines the cryo-EM structures of human myoferlin and dysferlin bound to Ca2+ and lipids, revealing how their domain dynamics enable membrane binding and fusion.

The paper uses molecular dynamics simulations to show cardiolipin increases membrane spontaneous curvature and reduces bending rigidity, leading to lateral heterogeneities through curvature sensing.

The paper introduces xMAS Builder, a tool that constructs atomistic membrane models for MD simulations using experimental data, and demonstrates its ability to generate stable models with insights into membrane packing density.

The paper analyzes ergosterol metabolism in yeast to show how successive modifications balance lipid ordering and fluidity for membrane phase separation.

The paper develops a method to engineer lipid-binding probes by screening protein-lipid interactions and identifies structural features that enhance binding to phosphatidylinositol phosphates.

This paper develops a thermodynamic model of non-dilute surface binding and phase separation for membrane-binding proteins and applies it to tight junction formation.

This study investigates how SARS-CoV-2 fusion peptide fragments interact with model membranes, showing the LLF motif's role in membrane fusion and how lipid composition affects fusogenic activity.

This study uses coarse-grained simulations to analyze OMP-phospholipid interactions and water dynamics in E. coli outer membranes.

This paper applies constant-pH MD simulations to study pH-dependent behavior of cationic ionizable lipids in lipid nanoparticles, focusing on their apparent pKa and implications for drug delivery.

The paper compares the reduced volume of plasma membrane vesicles and PC-cholesterol vesicles to infer their spontaneous curvature using different asymmetry conditions.

This study optimizes the modified cDICE method to control GUV size distribution by adjusting rotation time and angular frequency.

The paper uses a computational model to study how archaeal membrane lipids balance stability and flexibility, finding that adding bilayer lipids to bolalipid membranes achieves both.

The paper uses chemically defined lipid diets to study how lipidome complexity affects membrane function and cell phenotypes in minimal cells.

This study uses simulations to show that TMEM16 proteins primarily scramble lipids in an open groove when Ca2+ is bound, but also do so at the dimer interface when Ca2+ is absent.

This study investigates lipid diffusion in bilayers and monolayers, finding that water model properties significantly affect dynamics and suggesting the need for lipid model modifications.

This study uses single-molecule tracking to examine how disrupting the cytoskeleton affects plasma membrane receptor dynamics in plant cells, revealing distinct effects of actin and microtubule disruption.

This study uses molecular dynamics simulations to compare phosphatidylcholine membrane properties between pure and cancer model membranes, finding differences in surface area, chain order, and bilayer thickness.

The paper introduces a solvatochromic probe, C3L, to measure membrane order at the plasma membrane leaflet level, revealing asymmetry and local modulation by transbilayer interactions.

The paper uses calcium-induced hemifusion to create asymmetric GUVs and studies phase behavior changes, finding variability in outer leaflet exchange and correlation with asymmetry.

This study investigates the structural and functional dynamics of Staphylococcus aureus α-hemolysin during membrane interaction, revealing its role in cell necrosis and pore formation.

The paper uses imaging surface plasmon resonance and fluorescence correlation spectroscopy to study toxin-glycolipid interactions and their lipid dependencies on artificial and cellular membranes.

DIB-BOT is an open-source hardware system for rapid, reproducible DIB fabrication, reducing errors and increasing yield compared to manual methods.

This study shows that the conformational changes of the bacterial ABC transporter BmrA are influenced by membrane curvature, affecting its activity and conformational states.

This study investigates how Syt-7 C2 domains generate membrane curvature through electrostatic interactions and the wedge mechanism, using simulations and assays.

This study uses deep graph neural networks trained on MD simulations to predict phosphoinositide interaction sites on PH domains, achieving results comparable to simulations in seconds.

This study uses molecular dynamics simulations to show that membrane curvature affects lipid sorting and phase behavior in complex ways beyond traditional models.

This study identifies Dia2 as a regulator of lipid nanodomain organization at the plasma membrane, impacting JAK/STAT signaling and membrane protein activity.

This study identifies how Mycoplasma pneumoniae uses the protein P116 to transport lipids between membranes without ATP, revealing a simplified lipid uptake mechanism.

The paper shows seasonal changes in goldfish retinal neurons' membrane structure and excitability under constant environmental conditions, indicating internal seasonal regulation of CNS activity.