Reproductive tract infections research

The challenge of sequencing Chlamydia trachomatis and other bacterial STI genomes directly from clinical swabs: the optimum solution

This paper evaluates methods for sequencing bacterial STI genomes from clinical swabs, finding that target enrichment with a pan-genome panel is the most effective approach.

Chlamydia trachomatis genomes from rectal samples: description of new clade comprising ompA-genotype L4 from Argentina

This paper reports whole genome sequences of Chlamydia trachomatis from Argentina and Finland, identifying a novel ompA-genotype L4 clade and a non-LGV strain associated with anorectal proctitis.

TSST-1 promotes colonization of Staphylococcus aureus within the vaginal tract by activation of CD8+T cells

This study shows TSST-1 promotes S. aureus vaginal colonization by activating CD8+ T cells and altering the vaginal microbiota.

Effect of tryptophan starvation on inclusion membrane composition and chlamydial-host interactions

This study investigates how tryptophan starvation affects chlamydial inclusion membrane composition and host interactions during persistence.

Molecular and Pathological Characterization of Cervicovaginal Specimens Evince Significant Protection Against HPV Infections, Bacterial Vaginosis, and Epithelial Cell Abnormalities by Lactobacillus species

The paper analyzes cervicovaginal specimens to show that Lactobacillus species, particularly L. crispatus, protect against HPV infections, bacterial vaginosis, and epithelial abnormalities.

Lactobacillus crispatus S-layer proteins modulate innate immune response and inflammation in the lower female reproductive tract.

This study shows how Lactobacillus crispatus surface proteins modulate immune responses by shielding TLR ligands and interacting with anti-inflammatory receptors, reducing inflammation in the vaginal tract.

Vaginal metatranscriptome meta-analysis reveals functional BV subgroups and novel colonisation strategies

This study analyzes vaginal metatranscriptomes to identify functional subgroups and microbial strategies in bacterial vaginosis, highlighting functional differences over taxonomic ones.

Neisseria gonorrhoeae induces local secretion of IL-10 at the human cervix to promote asymptomatic colonization

This study shows Neisseria gonorrhoeae induces IL-10 secretion at the cervix via CEACAMs to prevent epithelial shedding and inflammation, enabling asymptomatic colonization.

Intranasal immunization with CPAF combined with cyclic-di-AMP induces a memory CD4 T cell response and reduces bacterial burden following intravaginal infection with Chlamydia muridarum

This study shows that intranasal immunization with CPAF and cyclic-di-AMP reduces bacterial burden in mice infected with Chlamydia muridarum by inducing memory CD4 T cell responses.

Geographic-specific Diversification of Vaginal Microbiota in China

This study analyzes vaginal microbiota diversity across five Chinese provinces, finding low alpha diversity, CST stability, and geographic variation in Lactobacillus crispatus prevalence.

The genital tract microbiota of IVF patients and oocyte donors: a comparative analysis

This study compares the genital tract microbiota and bacterial load between IVF patients and oocyte donors, finding minimal variation between groups and anatomical sites.

Evaluation in mice of cell-free produced CT584 as a Chlamydia vaccine antigen

This study evaluates CT584, a Chlamydia antigen produced via cell-free synthesis, as a vaccine in mice, finding it induces antibodies but not protection against infection.

Growth Analysis of Trichomonas vaginalis in Different Culture Media: Leveraging Large Language Models (LLMs) to Predict and Optimize In Vitro Growth Conditions

This study compares RSMT media with standard culture media to optimize Trichomonas vaginalis growth, finding RSMT significantly enhances growth by 85-250%.

Differential Drug Susceptibility Across Trichomonasvirus Species Allows for Generation of Varied Isogenic Clones of Trichomonas vaginalis

The paper uses 2CMC to clear TVV infections in Trichomonas vaginalis isolates, revealing differential drug susceptibility among TVV species and enabling the generation of isogenic clones.

The chlamydial transcriptional regulator Euo is a key switch in cell form developmental progression but is not involved in the committed step to the formation of the infectious form.

This study shows that ectopic expression of the chlamydial transcriptional regulator Euo arrests developmental progression at an early Intermediate Body stage but does not affect the formation of the infectious Elementary Body.

The Chlamydia effector IncE employs two short linear motifs to reprogram host vesicle trafficking

This study identifies two short linear motifs in Chlamydia effector IncE that reprogram host vesicle trafficking to support bacterial replication.

comparison of genetic diversity and phylogenetic structure of 18S gene of Trichomonas vaginalis in Hainan and Xinjiang provinces

This study compares genetic diversity and phylogenetic structure of 18S rRNA gene of Trichomonas vaginalis between Hainan and Xinjiang provinces, finding differences in haplotype and nucleotide diversity.

The Chlamydia trachomatis secreted effector CebN targets nucleoporins and Rae1 to antagonize STAT1 nuclear import

This study identifies CebN, a Chlamydia trachomatis effector that targets nucleoporins and Rae1 to disrupt STAT1 nuclear import, aiding bacterial survival.

Association of diet and inflammation with the vaginal microbiota of pregnant individuals with or without IBD

This study examines how diet and inflammation affect vaginal microbiota in pregnant individuals with or without IBD, finding that healthier diets correlate with higher Lactobacillus crispatus dominance.

Genomic insights into local scale evolution of ocular Chlamydia trachomatis strains within and between individuals in Gambian trachoma-endemic villages

This study analyzes genomic variation in Chlamydia trachomatis serovars A and B from Gambian trachoma-endemic villages to understand strain diversification and evolutionary pressures.

Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips

The paper shows that cervical mucus from Organ Chips protects vaginal chips from inflammation and injury caused by bacterial vaginosis microbiomes, suggesting potential biomarkers and therapeutic targets.

High-resolution functional description of vaginal microbiomes in health and disease

The paper develops metagenomic community state types (mgCSTs) to describe vaginal microbiomes by integrating composition and function, identifying associations with health outcomes and functional capabilities.

Variation of the vaginal microbiome during and after pregnancy in Chinese women

This study profiles the vaginal microbiome of Chinese women during pregnancy and 6 weeks postpartum, identifying variations linked to medical history and pregnancy outcomes.

Large-scale characterisation of the pregnancy vaginal microbiome and sialidase activity in a low-risk Chinese population

The paper characterizes the vaginal microbiome and sialidase activity in 2689 Chinese pregnant women, finding no association with preterm birth risk.

Vaginal Microbiome Topic Modelling of Laboring Ugandan Women With and Without Fever

This study uses microbial topic modeling to identify associations between vaginal microbiome composition and intrapartum fever in Ugandan laboring women, improving prediction beyond clinical variables.

Proteome-wide prediction of bacterial carbohydrate-binding proteins as a tool for understanding commensal and pathogen colonisation of the vaginal microbiome

The paper predicts bacterial carbohydrate-binding proteins (lectins) across genomes to explore their role in vaginal microbiome colonization and pathogenicity.

Whole-Genome Enrichment and Sequencing of Chlamydia trachomatis Directly from Patient Clinical Vaginal and Rectal Swabs

The paper improves whole-genome sequencing of Chlamydia trachomatis from clinical samples by redesigning an RNA bait library, achieving 100% efficiency and identifying genomic recombination in paired specimens.

Genomics of Ocular Chlamydia trachomatis after 5 years of SAFE interventions for trachoma in Amhara, Ethiopia

The paper analyzes genomic variation in Chlamydia trachomatis from Ethiopia to assess its role in continued transmission after SAFE interventions.

Optimal protocols for sequence-based characterization of the human vaginal microbiome

This paper evaluates best practices for sequencing-based characterization of the human vaginal microbiome, comparing 16S rRNA gene sequencing and shotgun metagenomic approaches.

Early transcriptional landscapes of Chlamydia trachomatis-infected epithelial cells at single cell resolution

The paper uses scRNA-Seq to analyze early host cell responses to Chlamydia trachomatis infection, identifying distinct transcriptional patterns and potential biomarkers.