Bacterial biofilms and quorum sensing

The c-di-GMP effector FleQ controls alginate production by repressing transcription of the algD gene in Azotobacter vinelandii

This study shows that FleQ, a c-di-GMP effector, represses algD transcription, controlling alginate production in Azotobacter vinelandii.

PqsE acts as an adaptor protein for the quorum-sensing transcription factor RhlR in Pseudomonas aeruginosa

This study shows that PqsE modulates RhlR activity in Pseudomonas aeruginosa through promoter-specific interactions, affecting gene expression and pathogenic behaviors.

Activation of the Pseudomonas aeruginosa Glycerol Regulon Promotes Antibiotic Persistence and Modulates Virulence Phenotypes

This study shows that loss of GlpR increases antibiotic resistance and biofilm formation in P. aeruginosa CF isolates, suggesting the glycerol regulon promotes persistence in the CF airway.

Phage lysates are reservoirs of biologically active quorum sensing signals

This study shows that phage lysates retain bioactive quorum sensing signals like PQS, which can influence bacterial behavior, independent of phage infection.

Infection dynamics and virulence potential of clinical Pseudomonas aeruginosa isolates in human airway epithelial models

The paper investigates infection dynamics and virulence of Pseudomonas aeruginosa in human airway epithelial models, identifying four infection clusters based on strain-specific virulence and host response.

Pseudomonas aeruginosa population dynamics in a vancomycin-induced murine model of gastrointestinal carriage

This study models Pseudomonas aeruginosa gastrointestinal carriage in mice using vancomycin pretreatment and identifies the stomach as a major barrier to colonization.

Comprehensive Genomic and Evolutionary Analysis of Biofilm Matrix Clusters and Proteins in the Vibrio Genus

This study analyzes the distribution, diversity, and evolution of biofilm matrix clusters and proteins across Vibrio species, revealing structural and evolutionary patterns of key genes.

Coumarins disrupt cell-cell communication for control of pathogenesis and virulence in ESKAPEEs and fungal opportunists

This study investigates how coumarins disrupt cell-cell communication in bacterial and fungal pathogens, revealing their potential to control pathogenesis and virulence.

Evolution of PqsE as a Pseudomonas aeruginosa-specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia

This study investigates the conservation and function of PqsE in Pseudomonas and Burkholderia, showing that PqsE in Pseudomonas enhances RhlR activity while Burkholderia orthologs do not.

Bacterial colony biopsies: spatial discrimination of heterogeneous cell types by cytometric fingerprinting

This study introduces a biopsy technique combined with cytometric fingerprinting to analyze spatial heterogeneity in bacterial colonies, revealing distinct physiological states across different locations.

Characterization and orthogonality assessment of two quorum sensing systems for synthetic biology applications

This study characterizes two quorum sensing systems and assesses their orthogonality to enable their simultaneous use in synthetic biology.

Single-cell bacterial culturing and antibiotic susceptibility testing using permeable hydrogel-shelled microcapsules

The paper introduces Bioflex, a microfluidic technique for isolating single bacterial cells in permeable microcapsules to study their responses to environmental changes like antibiotics.

Autoinduction expression systems by AHL-mediated quorum-sensing circuits in Synechococcus elongatus PCC 7942

This paper develops an AHL-mediated quorum-sensing circuit in Synechococcus elongatus to autoinduce gene expression based on population density, improving biomass recovery.

Role of Pseudomonas aeruginosa Dnr-regulated denitrification in oxic conditions

This study shows Pseudomonas aeruginosa consumes nitrate in oxic conditions, requiring Dnr and Dnr-regulated NorCB for detoxification, despite no growth advantage from nitrate.

The Quorum Sensing regulated sRNA Lrs1 is involved in the adaptation to low iron in Pseudomonas aeruginosa

This study identifies Lrs1, a quorum sensing-regulated sRNA in P. aeruginosa, as a post-transcriptional regulator of iron uptake and virulence under iron limitation.

Interplay of Virulence Factors and Signaling Molecules: Albumin and Calcium-Mediated Biofilm Regulation in Bordetella bronchiseptica

This study investigates how serum albumin and calcium inhibit biofilm formation in Bordetella bronchiseptica through two independent mechanisms involving c-di-GMP and adenylate cyclase toxin.

The quorum-sensing lexicon of Salmonella ameliorates acid stress in the host by a non-canonical mechanism

This study shows Salmonella Typhimurium uses AI-2 quorum sensing to enhance acid tolerance and survival in macrophages by modulating pH-sensing systems and SPI-2 regulators.

Exploring Quorum-Sensing-Based Bacterial GMOs for Addressing Bovine Mastitis: A Developmental Approach.

This study proposes a genetically modified bacterial approach using defensins and DNaseI to combat bovine mastitis without antibiotics, showing efficacy against biofilm formation and pathogen sensing.

Hyphal growth determines spatial organization and coexistence in a pathogenic polymicrobial community within alveoli-like geometries

This study shows that hyphal growth in microfluidic alveoli-like geometries enables coexistence of P. aeruginosa, S. aureus, and C. albicans by creating spatial organization that excludes competitors.

Matrix porosity is associated with Staphylococcus aureus biofilm survival during prosthetic joint infection

This study shows that Eap proteins in S. aureus biofilms reduce porosity and prevent macrophage invasion, enhancing biofilm survival during prosthetic joint infections.

Structural and functional analysis of Pseudomonas aeruginosa PelA provides insight into the modification of the Pel exopolysaccharide

This study determines the structure of PelA and shows it modifies Pel through deacetylation and hydrolysis, with PelAB interaction enhancing deacetylation and guiding export.

Regulatory effects on virulence and phage susceptibility revealed by sdiA mutation in Klebsiella pneumoniae

This study investigates the role of SdiA in K. pneumoniae virulence, showing it affects biofilm formation, phage susceptibility, and serum resistance, with C6-HSL modulating these effects.

A nutrient bottleneck limits antibiotic efficacy in structured bacterial populations

This study shows that nutrient availability limits antibiotic efficacy in structured bacterial populations by creating a traveling death front, which can be overcome or exacerbated by nutrient supply changes.

Biofilm-derived polymer networks constrain immune cell motility through adhesion-mediated physical interactions

This study shows that biofilm exopolysaccharides impede macrophage motility and directional persistence, using microfluidic models and simulations.

Individual-Based Modeling of Microbial Communities Integrating Genetic Mechanisms: A Case Study of LuxS-Mediated Quorum Sensing in Salmonella Typhimurium

The paper presents a digital twin framework for simulating microbial communities at the single-cell level, integrating genetic mechanisms to study quorum sensing and virulence in Salmonella Typhimurium.

Mutations in the Staphylococcus aureus Global Regulator CodY Confer Tolerance to an Interspecies Redox-Active Antimicrobial

This study identifies mutations in CodY that confer Staphylococcus aureus tolerance to pyocyanin, revealing metabolic suppression and enhanced oxidative stress response as protective mechanisms.

Spatial transcriptomics identifies novel Pseudomonas aeruginosa virulence factors

This study uses spatial transcriptomics to identify novel Pseudomonas aeruginosa virulence factors and host-pathogen interactions in ocular infection.

Transcriptional Signatures Associated with the Survival of Escherichia coli Biofilm During Treatment with Plasma-Activated Water

This study identifies transcriptional changes in E. coli biofilms exposed to plasma-activated water, revealing key genes and pathways involved in their survival and response to oxidative stress.

Spatial dynamics and emergent properties of pLS20 conjugation on solid surfaces

This study uses hybrid modeling to show spatial organization and quorum-sensing regulation significantly influence plasmid dissemination during bacterial conjugation on solid surfaces.

Achromobacter xylosoxidans modulates Pseudomonas aeruginosa virulence through a multi-target mechanism of competition

This study shows that Achromobacter xylosoxidans strain Ax 200 reduces Pseudomonas aeruginosa virulence in a zebrafish model through strain-dependent mechanisms affecting biofilm formation, motility, and iron acquisition.