Bacterial biofilms and quorum sensing

Quorum sensing employs a dual regulatory mechanism to repress T3SS gene expression

This study identifies a dual regulatory mechanism by LuxR in Vibrio campbellii that represses T3SS gene expression through transcriptional and post-translational repression of ExsA activity.

Lysyl-phosphatidylglycerol promotes cell-to-cell interaction and biofilm formation of Staphylococcus aureus as a biofilm matrix component

This study identifies lysyl-phosphatidylglycerol (Lys-PG) as a critical biofilm matrix component in Staphylococcus aureus that promotes cell-to-cell interactions and biofilm formation.

Galleria mellonella model for studying Gram-positive bacterial implant biofilms

This study evaluates the Galleria mellonella model for assessing biofilm formation by S. aureus and E. faecalis on implant materials, showing its effectiveness in mimicking clinical infections and antibiotic responses.

Proteomic characterisation of three independent series of sequential cystic fibrosis strains in an international Pseudomonas aeruginosa reference panel indicates positive selection in late infection strains.

The paper identifies proteins that show increased abundance in late-stage Pseudomonas aeruginosa isolates from CF patients, suggesting positive selection and adaptation to chronic infection.

Biofilm selection constitutively activates c-di-GMP synthesis by the bifunctional enzyme MbaA

The paper identifies mutations in the enzyme MbaA that enhance c-di-GMP synthesis, promoting biofilm formation in Vibrio cholerae by disrupting polyamine regulation.

Intracellular Pseudomonas aeruginosa persist and evade antibiotic treatment in a wound infection model

This study shows that Pseudomonas aeruginosa persists intracellularly in zebrafish wounds, evading antibiotics and host immunity, and can be re-sensitized by anti-biofilm agents.

The MutRS quorum sensing system controls lantibiotic mutacin production in the human pathogen Streptococcus mutans

This study identifies the MutRS quorum sensing system as a regulator of lantibiotic mutacin production in Streptococcus mutans and shows its role in inter- and intrastrain crosstalk.

Scanning Electron Microscopy Study of Bacterial Growth in Mycelial Extracellular Matrices

This study uses scanning electron microscopy to examine bacterial attachment to fungal hyphae in co-cultures, finding that EPS and ECM contribute to biofilm formation.

Spatial population dynamics of bacterial colonies with social antibiotic resistance

This study investigates spatial population dynamics of E. faecalis under antibiotic exposure, showing how cooperative interactions between resistant and sensitive strains create complex spatial structures that affect antibiotic response.

Dietary quorum quenching AHL lactonase impairs the adaptation of commensal bacterium to host innate immunity

The paper shows dietary quorum quenching AHL lactonase disrupts Aeromonas adaptation to host immunity, altering zebrafish microbiota composition through ecological competition with Plesiomonas.

Co-zorbs: Motile, multispecies biofilms aid transport of diverse bacterial species

The paper discovers co-zorbs, motile multispecies biofilms formed by Flavobacterium johnsoniae that transport other bacteria, revealing a new type of bacterial movement and cooperation.

Soft X-ray tomography reveals variations in B.subtilis biofilm structure upon tasA deletion

This paper uses soft X-ray tomography to analyze B.subtilis biofilm structure, showing structural changes in a tasA deletion mutant and the effect of TasA protein addition.

Quorum sensing regulation by the nitrogen phosphotransferase system in Pseudomonas aeruginosa

This study investigates how the nitrogen phosphotransferase system (PTSNtr) in Pseudomonas aeruginosa regulates quorum sensing, showing that PtsO and PtsN have distinct roles and regulons in controlling virulence gene expression.

The role of PilU in the surface behaviors of Pseudomonas aeruginosa

This study investigates the role of PilU in Pseudomonas aeruginosa surface behaviors by comparing wild-type and ΔpilU mutants, revealing altered twitching motility and colony formation.

Mapping of Quorum Sensing Landscape of Commensal and Pathogenic Staphylococci Reveals a Largely Inhibitory Interaction Network

This study maps quorum sensing interactions among staphylococcal species and identifies potent QS inhibitors, showing their potential as anti-virulence agents against MRSA.

Electrogenic Dynamics of Biofilm Formation: Correlation Between Genetic Expression and Electrochemical Activity in Bacillus subtilis

This study correlates genetic expression and electrochemical activity in Bacillus subtilis biofilms, showing electrochemical activity is present in biofilm-forming cells but not in biofilm-deficient mutants.

Illuminating microbial mat assembly: Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms

This study investigates how cyanobacteria and Chloroflexota cooperate in forming microbial mats through motility and biofilm formation.

Dynamics of biofilm-forming Bacillus subtilis in Caenorhabditis elegans gut

This study investigates B. subtilis biofilm formation in C. elegans gut, finding no biofilms in live worms but spores and diffused fluorescence, with biofilm formation occurring post-mortem.

Genetic dissection of cyclic di-GMP signaling in Pseudomonas aeruginosa via diguanylate cyclase disruption

This study disrupts all 32 GGDEF domain-containing proteins in P. aeruginosa to analyze c-di-GMP signaling, revealing residual c-di-GMP levels and the importance of functional overlap in bacterial signaling.

Breaking the Phalanx: Overcoming Bacterial Drug Resistance with Quorum Sensing Inhibitors that Enhance Therapeutic Activity of Antibiotics

This study identifies synergistic combinations of quorum-sensing inhibitors and antibiotics to combat antibiotic-resistant bacteria, finding that piperacillin with ketoprofen, indomethacin, and piroxicam shows broad antimicrobial action.

Quorum Sensing Regulates Virulence Factors in the Coral Pathogen Vibrio coralliilyticus

This study identifies an active quorum sensing pathway in Vibrio coralliilyticus that regulates virulence factors like proteases, biofilms, and type VI secretion systems.

The biofilm matrix protects Bacillus subtilis against hydrogen peroxide

This study shows that the biofilm matrix, particularly EPS and TasA fibers, protects Bacillus subtilis against hydrogen peroxide, with sporulation playing a minor role.

Pseudopaline-mediated zinc uptake by Pseudomonas aeruginosa determines specific clinically relevant phenotypes and infection outcome

This study shows that pseudopaline-mediated zinc uptake in Pseudomonas aeruginosa enables clinically relevant phenotypes and influences infection outcomes.

c-di-GMP is required for swarming motility in E. coli: a role for DgcO and colanic acid

This study shows that c-di-GMP is required for swarming motility in E. coli, with DgcO promoting colanic acid production, which has surfactant properties aiding surface navigation.

Surface remodeling and inversion of cell-matrix interactions underlies community recognition and dispersal in Vibrio cholerae biofilms

This study shows that Vibrio cholerae biofilms undergo surface remodeling and inversion of cell-matrix interactions, shifting from attractive to repulsive as biofilms age, which facilitates dispersal.

Human cell-derived extracellular vesicles exhibit anti-biofilm effects against Pseudomonas aeruginosa

This study shows human cell-derived extracellular vesicles reduce Pseudomonas aeruginosa biofilm formation, with effectiveness observed across multiple cell sources.

Chronic wounds and adaptive Pseudomonas aeruginosa: A phenotypic and genotypic characterization

This study examines Pseudomonas aeruginosa in chronic wounds, finding recurring genotypes with minor phenotypic and SNP differences, suggesting adaptation to the host environment.

Biofilm lifestyle as a common trait of ammonia-oxidizing archaea

The paper investigates biofilm formation in ammonia-oxidizing archaea, finding distinct colonization strategies and shared gene expression patterns related to biofilm growth.

Guided assembly of multispecies positive biofilms targeting undesirable bacteria

This study identifies beneficial microbial strains that form positive biofilms to exclude harmful bacteria and shows that compatibility control is needed in synthetic community design.

Engineering microbial consortia: uptake and leakage ratedifferentially shape community arrangement and composition

This study shows that amino acid uptake and leakage rates determine microbial community structure and composition in synthetic E. coli consortia.