RNA Interference and Gene Delivery

PEPsRNA is a database of experimentally tested peptide-based siRNA delivery systems, providing 2266 entries with analysis of peptides, siRNAs, and conjugates.

This study uses simulations to analyze LNP-mediated siRNA delivery, revealing pH-dependent structural changes and a mechanism for endosomal escape involving RNA-lipid interactions.

This paper develops a synergistic RNA switch system that enhances gene expression control by integrating multiple regulatory modules, achieving improved inducibility and reduced off-target effects.

The paper shows that glucose depletion enhances chloroquine-induced endosomal membrane damage in tumor cells, improving siRNA efficacy.

This paper develops a bacteriophage T4-based nanoparticle vector to deliver mRNA and protein payloads into primary human hematopoietic stem cells with up to 20% efficiency.

This study develops a polymer-lipid hybrid nanoparticle (PLNP) to enhance mRNA delivery and T cell immunity, showing improved antigen-specific T cell responses and protection against viral variants and cancer.

This study develops lung-targeted IL-10 mRNA lipid nanoparticles that reduce acute lung injury in a mouse model by attenuating inflammation and tissue damage.

This paper designs an mRNA-LNP vaccine by integrating RNA, lipid, and antigen engineering to achieve 90% protection in a murine model.

This study uses AF4-SAXS and other techniques to reveal structural heterogeneity in mRNA-LNP subpopulations, showing that N/P ratio affects cargo loading and transfection efficiency.

This paper introduces modified nucleosides in siRNA seed regions to enhance specificity and reduce off-target effects while maintaining on-target activity.

This study develops a lipid nanoparticle with endothelial cell membrane hybridization for targeted RNA delivery to treat ocular neovascular diseases, showing improved drug accumulation and safety in a mouse model.

This paper introduces PEG-free lipid nanoparticles stabilized with sodium triphosphate for RNA delivery, showing improved early expression and comparable safety in mice.

The paper identifies a novel regulatory mechanism involving proteolytic processing of N-terminal intrinsically disordered regions in C. elegans Argonaute proteins, which controls small RNA selectivity and activity.

The paper investigates the developmental dynamics and crosstalk between WAGO-1 and WAGO-3 in C. elegans, showing distinct regulatory roles and complex interactions with 22G-RNAs and 26G-RNAs.

This study develops CRISPR/Cas9 RNA lipid nanoparticles to clear HCMV infections, showing high efficacy and broad strain coverage.

SiaRNA predicts siRNA-mRNA efficacy using a Siamese Neural Network with bidirectional cross-attention, outperforming existing methods on benchmark datasets.

This paper develops hPep peptide-based nanoparticles for systemic mRNA delivery, showing effective tissue distribution and protein expression in mice and non-human primates.

This paper develops a gene expression system targeting mineralized tissues using bisphosphonate lipids, demonstrating enhanced RNA delivery and local therapeutic effects in bone regeneration and inflammation.

This paper develops a T cell-activating mRNA delivery platform that enables in vivo CAR T cell generation without exogenous stimulation or antibody conjugation.

This study identifies temporal mismatch between mRNA delivery and spermatogenesis as a barrier to functional rescue, showing that sustained expression aligned with developmental timing enables recovery in genetic infertility models.

This study develops anti-CD38 lipid nanoparticles for targeted mRNA delivery to multiple myeloma cells, improving tumor targeting and reducing hepatic accumulation.

This study shows hydroxychloroquine reduces inflammation from mRNA and viral-vector therapies without affecting their therapeutic effectiveness.

This study examines how mixing techniques and ethanol removal affect lipid nanoparticle (LNP) physicochemical properties using SAXS and computational fluid dynamics.

The paper compares the effects of eleven mixing techniques on mRNA lipid nanoparticle physicochemistry and biological performance, finding significant differences in particle properties and performance across methods.

This paper designs and evaluates RNA-loaded nanoparticles conjugated with short peptides to selectively target dendritic cells via Clec9A, improving immunotherapeutic selectivity and efficacy.

The paper develops a method to deliver collagen-encoding mRNA topically to improve skin condition by enabling in vivo collagen expression.

This paper demonstrates that modified self-amplifying RNA (saRNA) enables long-lasting protein expression in mouse and human brain tissues.

This paper evaluates siIL-1β-anti-CD44-Liposomes as an anti-inflammatory therapy, showing reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines in macrophages.

This study shows that anionic lipids in mRNA-LNPs can modulate immune responses, reducing inflammation and disease in a multiple sclerosis mouse model.

This paper develops DART, a bacterial nanosyringe system for delivering RNA and gene editors, demonstrating its ability to deliver various RNAs and knockout GFP in vitro and in vivo.