RNA Interference and Gene Delivery

This paper engineers protein nanocages to package both RNA and protein in vivo, demonstrating their ability to self-assemble and deliver multiple functional RNAs along with proteins.

This study identifies SID-4 as nck-1 in C. elegans and shows it is required for dsRNA import and systemic RNAi, with interactions and functional redundancy with SID-3.

This study identifies GLH proteins' role in controlling Argonaute pathway specificity and promoting transgenerational silencing in C. elegans by binding to target mRNAs and amplifying small RNAs.

This study shows that N1-methylpseudouridine-modified mRNA increases protein expression and reduces immunogenicity in human fibroblast-like synoviocytes.

This study designs effective siRNAs targeting SARS-CoV-2 structural proteins to silence their genes, identifying 4 siRNAs per gene with minimal off-target effects.

The paper identifies RAB18 as a key regulator that enhances GalNAc-conjugated siRNA efficacy in Hep3B cells by improving siRNA delivery and RISC access.

The paper investigates parental RNAi in Tribolium castaneum, showing dsRNA transmission to eggs and persistence through embryogenesis, while identifying evolutionary patterns of SID-1-like proteins.

This paper describes a method to detect and quantify antisense oligonucleotides in biological samples using splint ligation and quantitative PCR, showing sensitivity across multiple tissues and ASO types.

The paper investigates the role of two Piwi proteins in transgene-induced silencing in Paramecium, showing they have distinct but overlapping functions in generating and processing siRNAs for chromatin-level gene silencing.

The paper shows that a non-targeting shRNA control (SHC016) from the MISSION library causes cell death or growth arrest by targeting SNRPD3, highlighting off-target effects in RNAi studies.

The paper identifies two CSR-1 isoforms in C. elegans and shows they have distinct roles in fertility and immunity.

The paper shows that arginine methylation in CSR-1A enables specific binding of 22G-RNAs associated with spermatogenesis, distinguishing it from CSR-1B which binds oogenesis-related RNAs.

The paper shows that CSR-1 slicer activity and codon usage regulate 22G-RNA biogenesis in C. elegans germline mRNAs.

This paper develops an siRNA-nanoparticle therapy targeting SARS-CoV-2 that reduces viral load and improves survival in mice.

This paper develops a population-scale electroporation method for delivering nucleic acids to C. elegans, enabling efficient RNAi and CRISPR-based genetic targeting.

The paper shows that proteolytic processing of Argonaute proteins by DPF-3 regulates small RNA association, maintaining genome integrity and fertility in C. elegans.

PFRED is a software tool for designing and analyzing antisense oligonucleotides and siRNA, offering customizable design criteria and open-source access.

This paper develops a survivin-driven minicircle system for prostate cancer theranostics, showing it can detect aggressive tumors via urine and treat them with gene-directed therapy.

The paper maps ASO distribution and activity in the CNS of rodents and NHP after central administration, showing widespread effects across cell types.

This study identifies dsRNA processing requirements for efficient RNAi in Tetranychus urticae, showing that long dsRNA and membrane transport genes are critical for RNAi response.

This study demonstrates that gold nanoparticles coated with galactose can efficiently transport oligonucleotides across brain endothelial cells, enhancing transcytosis by 50-fold compared to free DNA.

The paper evaluates tissue-specific RNAi strains in C. elegans and identifies a suitable genetic background for constructing robust tissue-specific RNAi strains.

This study identifies factors regulating Mutator foci in C. elegans germline cells across developmental stages and cell cycle phases.

The paper develops a live-cell imaging assay to study how mRNA structures affect AGO2-siRNA interactions and mRNA degradation.

The paper identifies Xrn1p as a cofactor in the budding-yeast RNAi pathway, showing it enhances silencing through multiple functions.

This study evaluates splice-switching oligonucleotides (SSOs) in a mouse model of Erythropoietic Protoporphyria, showing that an SSO with a cholesteryl group increases correctly spliced FECH transcript by 80% in bone marrow.

The paper investigates the relationship between mRNA delivery timing and protein expression in lipid-based transfection, finding no intrinsic correlation at the single-cell level.

The paper shows that gene silencing via dsRNA in C. elegans neurons can occur in intestinal cells lacking RRF-1, requiring EGO-1 and revealing functional mosaicism.

The paper shows that P granules in C. elegans germ cells prevent RNA interference genes from being silenced by piRNAs, maintaining their expression.

This paper describes a method for spatio-temporally controlled gene manipulation in developing vasculature using lipid reagents and siRNA, showing successful knockdown of VE-cadherin without heart defects.