Advanced Electron Microscopy Techniques and Applications

MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

MagC enables the remote collection of ultrathin sections for volumetric correlative light and electron microscopy by using magnetic resin, improving reliability and reducing manual effort.

Cryo-EM Map of the CFA/I Pilus Rod

The paper determines the 4.0 Å resolution structure of CFA/I pili and confirms the role of proline 13 in structural stability using optical tweezers.

AutoCryoPicker: An Unsupervised Learning Approach for Fully Automated Single Particle Picking in Cryo-EM Images

AutoCryoPicker is an unsupervised method for automated single particle picking in cryo-EM images, improving accuracy and reducing bias compared to manual or supervised approaches.

3.1A structure of yeast RNA polymerase II elongation complex stalled at a cyclobutane pyrimidine dimer lesion solved using streptavidin affinity grids

The paper solves a high-resolution structure of a stalled RNA polymerase II elongation complex using streptavidin affinity grids to overcome air-water interface interactions.

Iron-sequestering nanocompartments as multiplexed Electron Microscopy gene reporters

The paper introduces a genetic iron-biomineralizing nanocompartment for multiplexed EM gene reporter imaging in mammalian cells.

A “Human-in-the-Loop” Approach for Semi-automated Image Restoration in Electron Microscopy

This paper introduces a human-in-the-loop framework for improving EM image quality through interactive image restoration and analysis.

High-throughput transmission electron microscopy with automated serial sectioning

This paper introduces a high-throughput TEM imaging platform with automated serial sectioning for generating detailed biological tissue datasets, particularly for brain circuit analysis.

A new cryo-EM system for electron 3D crystallography by eEFD

This paper introduces a new cryo-EM system for electron 3D crystallography called eEFD, showing improved data quality compared to 200 kV systems without energy filtration.

Electron cryo-tomography of vestibular hair-cell stereocilia

This paper develops a method to rapidly freeze unfixed stereocilia for 3D imaging, revealing structural features of stereocilia in a near-native state.

Magnetic Ultrathin Tissue Sections for Ease of Light and Electron Microscopy

The paper discusses the removal of a manuscript due to a ruling by ETH Zurich.

Sample preparation protocol enabling nano-to-mesoscopic mapping of cellular connectomes and their habitats in human tissues and organs

The paper provides a protocol for preparing human tissue samples for multibeam scanning electron microscopy to enable nano-to-mesoscopic mapping of cellular connectomes and their habitats.

Bayesian weighing of electron cryo-microscopy data for integrative structural modeling

The paper introduces a Bayesian scoring function to rank structural models using cryo-EM data and other information, validated on known complexes and implemented in an open-source platform.

Correlated Light-Serial Scanning Electron Microscopy (CoLSSEM) for ultrastructural visualization of single neurons in vivo

The paper introduces CoLSSEM, a method combining fluorescent labeling and electron microscopy to visualize synaptic connections in single neurons in vivo.

New tools for the analysis and validation of Cryo-EM maps and atomic models

The paper introduces new computational tools for validating cryo-EM maps and atomic models, including methods for resolution estimation, map sharpening, and map analysis.

FluoEM: Virtual labeling of axons in 3-dimensional electron microscopy data for long-range connectomics

FluoEM identifies multi-color fluorescent axons in 3D EM data without fiducial marks by matching neurite reconstructions from light microscopy.

Benchmarking cryo-EM single particle analysis workflow

The paper benchmarks cryo-EM single particle analysis workflows using a standard sample and procedures to ensure high-resolution map production.

Simultaneous Determination of Protein Structure and Dynamics Using Cryo-Electron Microscopy

This paper introduces a method to simultaneously determine protein structure and dynamics from cryo-electron microscopy data, applied to the STRA6 receptor.

Spotiton: New Features and Applications

The paper describes new features and applications of Spotiton, a cryoEM sample vitrification instrument, showing it produces high-quality specimens with no apparent sample damage.

Near-Atomic Cryo-EM Imaging of a Small Protein Displayed on a Designed Scaffolding System

The paper demonstrates that small proteins can be visualized at near-atomic resolution using cryo-EM when attached to a symmetric protein scaffold.

Exploring Applications of Crowdsourcing to Cryo-EM

The paper explores using crowdsourcing for cryo-EM particle picking, showing its potential and limitations through experiments with untrained citizen scientists.

An open-source cryo-storage solution

The paper presents an open-source cryo-storage system to improve sample storage efficiency and organization in cryo-EM laboratories.

The Cryo-Electron Microscopy Structure of the Type 1 Chaperone-Usher Pilus Rod

The paper presents the cryo-EM structure of the type 1 chaperone-usher pilus rod at 4.2 Å resolution, revealing its spring-like biomechanical properties.

EMHP: An accurate automated hole masking algorithm for single-particle cryo-EM image processing

The paper introduces EMHP, an automated hole masking algorithm for single-particle cryo-EM image processing to streamline data treatment.

Quantitative analysis of 3D alignment quality: itsimpact on soft-validation, particle pruning andhomogeneity analysis

This work introduces a soft-alignment validation method to assess alignment precision, accuracy, and data homogeneity in cryo-EM single particle analysis, improving map resolution through data quality enhancement.

MRCZ - A proposed fast compressed MRC file format and direct detector normalization strategies

The paper proposes a compressed MRC file format and normalization strategies for direct electron detectors to improve data storage and reduce noise in transmission electron microscopy.

Helical reconstruction in RELION

RELION 2.0 adds empirical Bayesian helical reconstruction with orientation priors, filament-level half-sets and local twist/rise optimisation, yielding near-atomic maps for four filament datasets.