Advanced Electron Microscopy Techniques and Applications

Low-pass filtering revisited: ramped phase randomization for cryo-EM map comparison and data augmentation

This paper introduces ramped phase randomization to improve cryo-EM map comparison and data augmentation by reducing fine-scale detail while preserving low-frequency content and enhancing machine learning performance.

Cryo-EM structures of the YsaN ATPase reveal dodecameric and tetradecameric assemblies in the Yersinia enterocolitica T3SS

The paper reports cryo-EM structures of YsaN ATPase in dodecameric and tetradecameric states, revealing structural dynamics and mechanisms of oligomer assembly and function in the T3SS.

Agentic cryo-EM structure determination with CryoPilot

CryoPilot uses two language model agents to automate cryo-EM structure determination, improving resolution and model accuracy through adaptive decision-making.

Femtosecond pulsed laser ablation for in-tissue electron cryo-tomography

This paper demonstrates that cryogenic femtosecond pulsed laser ablation can efficiently prepare vitreous specimens of mammalian brain tissue for cryo-ET, enabling routine in situ cell biology of multicellular samples.

Preserving Information and Integrity in Cryo-EM: A Fourier-Space Deposition Approach

The paper identifies limitations in current cryo-EM data deposition practices and proposes an alternative approach to better preserve information and support validation.

Recovery of a minor cryo-EM particle population reveals conformational equilibria linking cofactor loading, turnover, and reactivation in methionine synthase

The paper reanalyzes a cryo-EM dataset using advanced methods to uncover a minor reactivation-state structure of methionine synthase, revealing conformational changes during cofactor turnover.

Correcting Multiple Scattering Enables Dynamical Electron Crystallography in Standard Workflows

GROND improves electron crystallography by correcting multiple scattering, enhancing refinement accuracy and revealing hidden structural details in various materials.

Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching

This paper improves cryo-EM reconstruction of small complexes using 2D template matching and high-resolution structures as priors, enhancing density of ligand-binding sites in a 43 kDa protein kinase.

Replacing H2O with D2O in cryo-EM reduces radiation damage in biological samples

This study shows that replacing H2O with D2O in cryo-EM increases radiation tolerance in protein crystals by reducing recoil energy and bond breakage.

Rapid femtosecond laser trenching accelerates cryo-electron tomography lamella workflows

This paper introduces a method using femtosecond laser trenching to speed up cryo-electron tomography lamella preparation by reducing milling time and damage.

Diffractograph: single-particle reconstruction using electron-diffraction data

Diffractograph reconstructs 3D structures from single-particle electron diffraction data by selecting compatible frames and determining orientations, achieving accurate results on synthetic data.

Fluorescent Biosensors for Cryogenic Correlative Light and Electron Microscopy

The paper develops fluorescent biosensors for cryogenic correlative light and electron microscopy to measure physiological conditions alongside structural data.

Super-Resolution Processing Enables In Situ Subtomogram Averaging Beyond the Nyquist Limit

This paper demonstrates that super-resolution processing of electron-event representation data can recover biologically meaningful information beyond the physical Nyquist limit in in situ subtomogram averaging.

Workflow and instrumentation for multiscale correlative imaging in voluminous cryogenic specimens

This paper introduces a multiscale correlative imaging workflow for cryogenic specimens, enabling fluorescence-guided site preparation and targeted lamellae lift-out for cryo-ET.

In situ cryo-EM reveals how a flagellar motor transmits high torque

This study uses in situ cryo-EM to reveal how the flagellar motor in Borrelia burgdorferi transmits high torque through mechanical coupling and a split washer-shaped FrbA brace.

A learning-to-rank framework for large-scale identification of unknown proteins from cryo-EM and cryo-ET maps

CryoAssign identifies unknown proteins from cryo-EM and cryo-ET maps using a learning-to-rank framework that prioritizes correct candidates.

Ab initio CryoEM maps minimizing preferred orientation problems in heterogeneous mixtures: ReconSIREN

ReconSIREN is a deep learning framework that improves CryoEM map accuracy by addressing preferred orientation errors in heterogeneous samples.

The CAHRA Challenge: A Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms

The paper introduces the CAHRA Challenge, a community-wide assessment of cryo-EM heterogeneous reconstruction algorithms using three benchmark datasets.