Advanced Electron Microscopy Techniques and Applications

Preserving Information and Integrity in Cryo-EM: A Fourier-Space Deposition Approach

The paper identifies limitations in current cryo-EM data deposition practices and proposes an alternative approach to better preserve information and support validation.

Recovery of a minor cryo-EM particle population reveals conformational equilibria linking cofactor loading, turnover, and reactivation in methionine synthase

The paper reanalyzes a cryo-EM dataset using advanced methods to uncover a minor reactivation-state structure of methionine synthase, revealing conformational changes during cofactor turnover.

Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching

This paper improves cryo-EM reconstruction of small complexes using 2D template matching and high-resolution structures as priors, enhancing density of ligand-binding sites in a 43 kDa protein kinase.

Gold Electron Microscopy Grids with Anisotropic Foil Geometry Enable On-Grid Contact Guidance

This paper introduces a wafer-scale fabrication method for all-gold EM grids that enable on-grid actin organization through anisotropic foil geometry without chemical micropatterning.

High-resolution cryoEM of nucleosomes in nuclear extracts of mammalian cells

The paper presents a method for high-resolution cryoEM of nucleosomes in mammalian cell nuclear extracts without requiring thin sectioning.

FPGA-based scanner and SerialEM server for 4D-STEM Electron Tomography

The paper develops an FPGA-based scanner and SerialEM server for 4D-STEM electron tomography, enabling automated complex protocols and demonstrating system performance with a mitochondrial tomogram.

Cryo-EM Sample Preparation with Soft-Landing and Laser Flash Melting

This paper demonstrates a method to rehydrate proteins after soft-landing for cryo-EM by flash melting, enabling native configurations and improving sample preparation.

MemBrain v2: an end-to-end tool for the analysis of membranes in cryo-electron tomography

MemBrain v2 couples a generalist U-Net cryo-ET membrane segmenter, a mesh-based picker reaching 91% F1 for photosystem II from one annotated membrane, and geodesic particle statistics.

Volume Electron Microscopy of Cortical Organoids: Methods for Region Identification, Connectome Reconstruction, and Organelle Segmentation

This paper establishes a workflow for applying volume electron microscopy to human cortical organoids, enabling micro-connectomic reconstruction at nanometer resolution.

Cryoprotectants-assisted plunge freezing of thick brain tissue specimens for targeted physiologically relevant cryo-imaging in situ

This paper develops a cryoprotectant-assisted plunge freezing method for preserving thick mouse brain tissue for cryo-imaging, enabling high-resolution visualization of cellular structures and validating their physiological relevance.

In-cell discovery and characterization of a non-canonical bacterial protein translocation-folding complex

The paper identifies and characterizes a non-canonical bacterial protein translocation-folding complex using cryo-electron tomography, proteomics, and structure prediction in Mycoplasma pneumoniae.

Overcoming air-water interface induced artifacts in Cryo-EM with protein nanocrates

This paper introduces a method to reduce air-water interface artifacts in cryo-EM by encapsulating proteins in hydrophilic shells, demonstrating its effectiveness with three protein examples.

Snapshots of Internal Protein Crystal Architecture at the Nanoscale

This study uses 4D-STEM to visualize nanoscale dynamics in protein microcrystals, revealing continuous rearrangements and radiolytic damage propagation.

Lessons learned from a Kaggle challenge for particle picking in cryo-electron tomography

The paper describes a Kaggle challenge for particle picking in cryo-electron tomography, resulting in improved algorithms and highlighting the need for better annotation quality measures.

Characterization of energy filtering slit widths for MicroED data collection

This study evaluates energy filter slit widths in MicroED to improve signal-to-noise ratio and data quality for macromolecular structure determination.

Near-atomic in-situ architecture and membrane-coupled dynamics of the Vibrio cholerae sheathed flagellum

This study determines the near-atomic structure of Vibrio cholerae's sheathed flagellum and reveals its dynamic architecture and membrane-coupled mechanisms.

FlyTomo: A streamlined software for on-the-fly cryo-ET data processing and diagnosis

FlyTomo streamlines cryo-ET data processing and diagnosis, enabling real-time feedback and structured data management for improved structural biology research.

Streamlined Montage Cryo-Electron Tomography for Exploring the Ultrastructure of Cells and Tissues

This paper introduces a streamlined workflow for montage cryo-electron tomography that simplifies data acquisition and processing, enabling high-throughput, nanoscale-resolution imaging of biological samples.

LowDoseWizard - rapid and standardised setup of low-dose cryo-TEM imaging in SerialEM

LowDoseWizard streamlines low-dose cryo-TEM setup in SerialEM, reducing setup time and improving reproducibility for high-resolution cryo-EM data collection.

Confidence-guided cryo-EM map optimisation with LocScale-2.0

This paper introduces LocScale-2.0 and LocScale-FEM to improve cryo-EM map quality by enhancing local detail while preserving weak structural context and providing confidence scores for interpretation.

WebCalEM: a browser-based tool for routine and accurate pixel size calibration in cryo-EM

WebCalEM provides a browser-based tool for accurate pixel size calibration in cryo-EM, eliminating installation barriers and enabling routine use.

Interactive segmentation of membrane and membrane mimic densities in cryo-EM maps

The paper introduces SURFER, a tool for segmenting membrane and membrane mimic densities in cryo-EM maps to improve analysis and visualization.

IsoNet2 determines cellular structures at submolecular resolution without averaging

IsoNet2 reconstructs high-resolution 3D cellular structures from cryogenic electron tomography without averaging.

EasyGrid: A versatile platform for automated cryo-EM sample preparation and quality control

EasyGrid automates cryo-EM sample preparation and quality control, improving vitrification for macromolecules and mammalian cells.

Asymmetric architecture and adaptation of Treponema flagella

This study determines the asymmetric structure of Treponema denticola flagella and shows how it enables efficient motility.

Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules

This study investigates whether pulsed electron beams reduce radiation damage in cryo-EM and finds no significant difference compared to conventional illumination.

CryoWriter: A Robotic Solution for Improved Cryo-EM Grid Preparation

The paper introduces a robotic system for cryo-EM grid preparation that improves sample efficiency and reconstruction quality.

Affinity purification contaminants identified by cryo-EM and mass spectrometry

The paper identifies two protein contaminants from affinity-purified samples using cryo-EM and mass spectrometry, emphasizing the importance of resin specificity in purification.

AlignPCA-2D: PCA-Reduced Euclidean Vector Alignment for 2D Classification in Cryo-EM

AlignPCA-2D improves 2D classification in cryo-EM by using PCA-reduced Euclidean vector alignment, achieving competitive accuracy with lower computational cost.

AreTomoLive: Automated reconstruction of comprehensively-corrected and denoised cryo-electron tomograms in real-time and at high throughput

AreTomo3 and DenoisET form an automated GPU pipeline that reconstructs cryo-electron tomograms with tile-based contrast transfer function correction, screens their quality, and denoises them during acquisition.