Poxvirus research and outbreaks

Mpox mRNA-1769 Vaccine Inhibits Orthopoxvirus Replication at Intranasal, Intrarectal, and Cutaneous Sites of Inoculation

The paper shows that an mRNA vaccine against mpox reduces virus replication at multiple inoculation sites and provides protection with a single dose, supported by antibody-mediated immunity.

Experimental monkeypox virus infection in rats

This study inoculates rats with MPXV, observing skin lesions and viral shedding, suggesting rats may play a role in MPXV transmission.

Poxviruses induce a nuclear environment in the cytoplasm to promote viral replication

This study investigates the cellular response to poxvirus infection, showing that MVA activates innate immune genes, while Camelpoxvirus activates NF-kB signaling, and poxviruses induce nuclear remodeling by transporting nuclear proteins into viral factories.

Large scale semi-automated quantification of viral spread reveals multi-phasic structure of plaque formation

This study uses live-cell imaging to track viral plaque formation in real-time, revealing three distinct phases and variability in replication kinetics across cell lines.

Preliminary report on the molecular epidemiology of mpox, South Africa 2024

This paper reports on mpox cases in South Africa in 2024, finding that the virus was reintroduced and transmitted locally, clustering with known outbreak variants.

Molecular basis for the host range function of the poxvirus PKR inhibitor E3

This paper identifies species-specific interactions between poxvirus PKR inhibitors E3 and K3 and host PKR proteins, challenging the sequestration model for E3 function.

Regulation of Necroptosis and the Type Interferon Response by Monkeypox/mpox Virus.

This study investigates how MPXV evades necroptosis and the type I interferon response by modifying key proteins, showing that it inhibits necroptosis through RIPK3 degradation and MLKL inhibition, and is sensitive to interferon via ZBP1, RIPK3, and MLKL.

Molecular Architecture of Monkeypox Mature Virus

This study uses cryo-ET and STA to reveal the architecture of mature MPXV particles and identifies irregular virions with distorted core walls.

Genomic analyses of recently emerging clades of mpox virus reveal gene deletion and single nucleotide polymorphisms that correlate with altered virulence and transmission

This study analyzes genomic differences in emerging mpox virus clades, identifying deletions and mutations linked to altered virulence and transmission.

Sero-epidemiological investigation and risk factors associated with camelpox in pastoral areas of Somali region, eastern Ethiopia: a cross-sectional study

This study estimates camelpox seroprevalence and identifies risk factors in Somali region, Ethiopia, finding higher infection rates in females, young camels, and during the rainy season.

Comprehensive Mutational Landscape Analysis of Monkeypox Virus Proteome

This study analyzes the mutational landscape of the Monkeypox virus proteome using ESCOTT and provides a resource for assessing new variants.

MPXV Infects Human PBMCs in a Type I Interferon-Sensitive Manner

This paper shows that MPXV infects human PBMCs in a type I interferon-sensitive manner, with monocytes, cycling NK cells, and regulatory CD4+ T-cells as susceptible cell types.

Mpox virus (MPXV) vertical transmission and fetal demise in a pregnant rhesus macaque model

This study infects pregnant rhesus macaques with clade IIb Mpox virus and finds vertical transmission to the fetus and adverse pregnancy outcomes.

Exploring the Transcriptomic Profile of Human Monkeypox Virus via CAGE and Native RNA Sequencing Approaches

This study uses CAGE and long-read RNA sequencing to analyze the transcriptional architecture of human monkeypox virus, identifying alternative TSSs, TESs, promoter elements, and novel genes.

Genomic sequence analysis of the first mpox virus detected in Kenya

The paper reports the genomic analysis of the first mpox virus case in Kenya, showing it clusters with clade Ib strains linked to the DRC outbreak and highlights the need for expanded sequencing efforts.

Discovery of a Heparan Sulfate Binding Domain in Monkeypox Virus H3 as an Anti-poxviral Drug Target Combining AI and MD Simulations

The paper identifies a conserved heparan sulfate binding domain in MPXV H3 using AI and MD simulations, validates its role in viral adhesion, and demonstrates a protein inhibitor that disrupts this interaction, inhibiting infection in vitro and in vivo.

Genomic Epidemiology of Monkeypox Virus During the 2022 Outbreak in New York City

This study analyzes MPXV genomic data from NYC during the 2022 outbreak, identifying NYC-specific lineages and estimating multiple infections, while highlighting gaps in epidemiological-genetic concordance.

Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus

The paper presents the 3.8 Å cryo-EM structure of the VACV DNA polymerase holoenzyme and shows its structural similarity to MPXV, with transient E9-D4 interactions and conformational flexibility.

Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores

The paper uses cryo-EM and AlphaFold to determine the structure of the poxvirus core, identifying A10 trimers as the key component of the palisade layer.

THE PALISADE LAYER OF THE POXVIRUS CORE IS COMPOSED OF FLEXIBLE A10-TRIMERS

The paper identifies the palisade layer of the vaccinia virus core as composed of flexible A10 trimers using cryoET, STA, and AlphaFold2.

Genomic characterization of the recent monkeypox outbreak

The paper analyzes Monkeypox virus genomes from the 2022 outbreak and compares them to historical and related sequences to determine their origin and genetic characteristics.

Genomic characterization and molecular evolution of human Monkeypox viruses

This study analyzes 604 human Monkeypox virus genomes to examine genomic epidemiology and molecular evolution, identifying synteny breaks, selection pressures, and phylogenetic assignments to the West African clade.

Comparative genomics and integrated system biology approach unveiled undirected phylogeny patterns, mutational hot spots, functional patterns and molecule repurposing for monkey pox virus

The paper analyzes MPXV genomes and host-virus interactions to identify mutational hotspots, functional patterns, and potential drug repurposing targets for monkeypox treatment.

Clade-Specific MPXV PCR Assays

The paper presents clade-specific PCR assays for mpox virus detection, improving accuracy and enabling clade identification during outbreaks.

Drug combinations proposed by machine learning on genes/proteins to improve the efficacy of Tecovirimat in the treatment of Monkeypox: A Systematic Review and Network Meta-analysis.

This study uses machine learning to identify drug combinations that improve Tecovirimat efficacy for Monkeypox, showing significant reductions in p-values for treatment scenarios.

The genome sequence of the Lumpy Skin Disease virus from the outbreak in India suggests a distinct lineage of the virus

The paper reports genome sequences of Lumpy Skin Disease Virus from an outbreak in India, finding a distinct lineage with significant genetic variations compared to the reference genome.

MpoxRadar: a worldwide Mpox genomic surveillance dashboard

MpoxRadar tracks Mpox virus mutations globally through an interactive dashboard, allowing users to analyze mutation profiles and filter data by various parameters.

Evolutionary potential of the monkeypox genome arising from interactions with human APOBEC3 enzymes

The paper analyzes APOBEC3-driven mutagenesis in monkeypox virus genomes, finding over-representation of TC hotspots and under-representation of GC hotspots, suggesting potential for future human APOBEC3-mediated evolution.

A distinct phylogenetic cluster of Monkeypox genomes suggests an early and cryptic spread of the virus

The paper identifies a distinct phylogenetic cluster of monkeypox virus genomes, lineage A.2, which differs from the dominant lineage B.1 associated with the 2022 European outbreak.

Genomic clustering and sequence context of mutations in human monkeypox virus (hMPXV1) genomes

The paper analyzes mutations in hMPXV1 genomes to identify clustering patterns and sequence context, suggesting APOBEC-mediated editing as the mutational mechanism.