Poxvirus research and outbreaks

Antigen-agnostic identification of poxvirus broadly neutralizing antibodies targeting OPG153

The paper identifies OPG153 as a target of broadly neutralizing antibodies against MPXV and related viruses using antigen-agnostic isolation, AlphaFold 3 modeling, and cryo-EM.

Lower pathogenicity but similar genital shedding of monkeypox virus clade Ib compared to clade Ia in a rodent model

Structure and functional analyses of Vaccinia virus entry- fusion complex component J5 protein

The paper determines the NMR structure of J5 protein and identifies functional residues critical for EFC integrity and membrane fusion in Vaccinia virus entry.

Ciclopirox suppresses poxvirus replication by targeting iron metabolism

This study identifies ciclopirox as an antiviral agent that suppresses poxvirus replication by targeting host iron metabolism.

Evolutionary Variation of Poxvirus Genome Architecture

This study analyzes poxvirus genome architecture across the family, finding correlations between genome size, coding density, GC-content, and protein distribution, with implications for viral evolution and biology.

ISG15 Differentially Modulates Clade Ib and II MPXV Infection in MEF cells

This study shows ISG15 modulates Clade Ib and II MPXV infection differently in MEF cells, with clade-specific effects on viral replication and immune evasion.

Enhanced mouse virulence of mpox virus clade Ib over clade IIb despite genomic changes caused by human-to-human transmission

This study compares the virulence of mpox virus clade Ib and IIb, finding clade Ib is more virulent in mice despite genomic changes from human-to-human transmission.

High genetic diversity of mpox virus (MPXV) in three different rodent species in the Democratic Republic of the Congo (DRC)

The paper identifies MPXV diversity in three rodent species in the DRC, showing multiple clades and proximity to human populations.

In situ cryo-electron tomography of vaccinia virus exit from infected cells

This study uses cryo-electron tomography and SIM to analyze vaccinia virus egress, revealing septin and clathrin roles in viral spread and release.

Comment on "Inverted repeats in the monkeypox virus genome are hot spots for mutation"

The paper reanalyzes MPXV mutation data and finds no statistically significant enrichment of APOBEC-signature mutations in cruciform structures formed by inverted repeats.

Structural Dissection of Vaccinia G9 Identifies Residues Essential for Membrane Fusion and Complex Assembly

This study identifies critical residues in the Vaccinia virus G9 protein essential for EFC function and membrane fusion, revealing conserved structural motifs across poxviruses and Nucleocytoviricota.

Highly efficient anogenital transmission of clade Ia mpox virus associated with increased shedding.

This study investigates mpox virus transmission in prairie dogs, finding that anogenital mucosal routes lead to high shedding and efficient transmission.

Development of a replication-defective MPXV platform for fundamental and therapeutic research

This paper develops a replication-defective MPXV platform for antiviral research and virology studies by engineering particles that recapitulate key viral life cycle stages without requiring high-containment facilities.

Non-Redundant Roles of Topoisomerase 2α and 2β in the Cytosolic Replication of Vaccinia Virus

This study identifies distinct roles of TOP2A and TOP2B in vaccinia virus replication, showing TOP2A promotes replication while TOP2B suppresses it by forming antiviral granules.

Pathogenicity and transmissibility of Monkeypox virus in dormice

This study evaluates MPXV pathogenicity and transmissibility in African dormice, finding high susceptibility, lethal infections, and both direct and limited airborne transmission.

Establishing a Digital PCR-Based Reference Measurement Procedure for Monkeypox Virus: An Inter-laboratory Assessment and Standardization Study

This paper establishes a ddPCR-based reference measurement procedure for MPXV quantification, demonstrating high precision and inter-laboratory consistency.

Potent Neutralization by Antibodies Targeting the Mpox A28 Protein

The paper identifies and characterizes monoclonal antibodies targeting the Mpox A28 protein, showing potent neutralization of Mpox and Vaccinia virus through complement-dependent mechanisms and providing protection in mice.

Evaluating the efficacy of LUMPY-NIL herbal powder, dermal Spray and dermal ointment in treating Lumpy Skin Disease (LSD) in Bovines

This study evaluates a polyherbal treatment for Lumpy Skin Disease in cattle, showing reduced lesion size, resolved systemic symptoms, and partial milk yield recovery within 15 days.

Antibody Binding and Neutralizing Targets within the Predicted Structure of the Poxvirus Multiprotein Entry-Fusion Complex

The paper identifies two novel targets in the poxvirus EFC that induce cross-neutralizing antibodies and offers insights into immune evasion strategies.

Drug screen reveals new potent host-targeted antivirals against Mpox virus

The paper identifies potent host-targeted antivirals against Mpox virus through high-throughput screening of a kinase inhibitor library, showing efficacy in vitro and in a mouse model.

Primary bovine white blood cells support dissemination of Lumpy Skin Disease Virus while suppressing viral replication

This study shows that primary bovine white blood cells support LSDV dissemination but suppress viral replication, with heat-treated virus inducing antiviral gene expression.

Novel derivatives of BCV and (S)-HPMPA inhibit orthopoxviruses and human adenoviruses more potently than BCV

This study evaluates novel prodrugs based on (S)-HPMPA and (S)-HPMPC, finding that ODE-(S)-HPMPA formate is more potent against orthopoxviruses and adenoviruses than BCV, but less effective against HSV-1.

The 2.3 A Structure of A21, a Protein Component of the Conserved Poxvirus Entry-Fusion Complex

The paper determines the crystal structure of A21, a poxvirus EFC protein, revealing its conserved fold and interactions within the entry-fusion complex.

Stability and characterization of infectious monkeypox virus extracellular virions in vitro2and vivo; implications for transmission, pathogenesis and treatment

This study characterizes the stability and infectious properties of monkeypox and vaccinia virus extracellular virions in vitro and in vivo, highlighting strain- and cell line-dependent variations in their behavior.

Attenuation of the 2022 global outbreak monkeypox virus relative to its clade IIb ancestor

This study compares a 2022 MPXV lineage B.1 virus with its clade IIb ancestor A.1, showing attenuated phenotypes including reduced virulence and immune evasion.

In vitro and in vivo Antiviral Activity of the Acyclic Nucleoside Phosphonate Prodrug LAVR-289 against Poxvirus and African Swine Fever Virus Replication

This study evaluates LAVR-289, a nucleoside analogue, showing antiviral activity against poxviruses and ASFV in vitro and in vivo with no toxicity.

IMMUNOGENICITY AND SAFETY OF CHICKEN EMBRYO FIBROBLAST ADAPTED FOWLPOX VACCINE

This study adapts fowlpox virus to chicken embryo fibroblasts, demonstrating higher titres and safety compared to a CAM-based vaccine.

Antiviral activity of Tecovirimat against Mpox virus clades 1a, 1b, 2a and 2b

This study evaluates Tecovirimat's antiviral activity against four Mpox virus clades and finds it effectively inhibits all at nanomolar concentrations.

Role of vaccinia virus growth factor in stimulating the mTORC1-CAD axis of the de novo pyrimidine pathway under different nutritional cues

This study identifies vaccinia virus growth factor (VGF) as a key factor enabling mTORC1-CAD axis activation in nutrient-poor conditions, enhancing de novo pyrimidine synthesis for viral replication.

The antiviral activity of licensed therapeutics against Mpox clade Ib, in vitro; alternative options for the treatment of Mpox

This study evaluates the antiviral activity of 12 therapeutics against Mpox clade Ib in vitro, finding that several licensed antivirals and anti-parasitic drugs show inhibitory activity.