Collagen: Extraction and Characterization

P4HA1 and P4HA2 accept both shared and isoenzyme-specific substrate residues in type I collagen to directly and indirectly orchestrate its post-translational modification landscape

This study investigates the substrate specificity of P4HA1 and P4HA2 in collagen post-translational modifications using proteomic and peptide analysis in mouse cells.

Enhancing Collagen Biosynthesis in Mammalian Cells Through Hypoxia-Mimetic Prolyl Hydroxylase Inhibition

This study inhibits prolyl hydroxylase to enhance collagen type I production in mouse cells under normoxia, achieving a 29-fold increase.

A recombinant heterotrimeric mini-procollagen I reveals new functional and structural roles for the α2(I) chain

This study produces and characterizes recombinant procollagen I variants to elucidate the functional role of the α2(I) chain in fibril formation and proteolytic processing.

Mechanical cues of an interpenetrating polysaccharide matrix regulate self-assembly of collagen fibers

This study shows that the viscoelastic properties of an alginate ECM regulate collagen fiber self-assembly through mechanical coupling.

Sequence-dependent Stability and the Apparent Two-state Thermal Transition of Extended Collagen Triple Helices

This study investigates how sequence-dependent stability influences the thermal behavior of extended collagen triple helices using recombinant collagen mimetic peptides.

Local sequence context determines the effect of glycine substitutions in collagen triple helices

The paper shows that glycine substitutions in collagen triple helices have varying effects based on local sequence context, with GP sites being especially restrictive.

Introducing non-enzymatic crosslinks into atomistic simulations of collagen fibrils

The paper extends the ColBuilder framework to include non-enzymatic AGE crosslinks in collagen fibril simulations, showing their distinct mechanical effects compared to enzymatic crosslinks.

Targeting collagen biosynthesis by small molecules inhibiting the function of the peptide-substrate-binding domain of collagen prolyl 4-hydroxylases

The paper develops a FRET-based assay to identify small molecules that inhibit collagen prolyl 4-hydroxylases by targeting their peptide-substrate-binding domain, leading to the discovery of a selective inhibitor.

The Molecular Origin of Water-Mediated Collagen Contraction

This study identifies sequence-dependent rules for collagen contraction driven by water-mediated structural rearrangements during dehydration.

Collagen-based bilayered biomimetic tubular materials for vascular and airway applications

This paper presents collagen-based bilayered tubular scaffolds for vascular and airway applications, demonstrating compliance and flexibility suitable for implants but with limitations in suture retention strength.

Collagen IV of basement membrane: V. Bromide-mediated sulfilimine bonds interlock the quaternary structure of NC1-hexamer of scaffolds enabling metazoan evolution.

The paper shows that sulfilimine bonds stabilize the quaternary structure of Col-IV121 hexamers across species and that bromide-mediated bond formation is evolutionarily conserved.

Relyophilized collagen scaffold to improve handling and small molecule loading

This paper introduces a second lyophilization step to improve collagen scaffold handling and small molecule loading efficiency for regenerative medicine.

P4HA3 is abundantly expressed during early embryonic development, but does not catalyze the formation of 4-hydroxyproline in type I collagen

Computational reconstruction of Tyrannosaurus rex collagen for biomanufacturing of paleo-inspired biomaterials with tuneable stiffness

This paper computationally reconstructs T. rex collagen using paleo-proteomics and protein models, enabling tunable biomaterial stiffness through inducible crosslinking.

Engineered Transdermal Peptide-Recombinant Type III Collagen Hydrogel with Biological Efficacy in Cell Proliferation and Wound Healing

This study develops a transdermal peptide-recombinant type III collagen hydrogel with high purity and structural stability, showing enhanced wound healing effects in cell and mouse experiments.

Overglycosylation introduces local changes in triple helix alignment in collagen type I fibril structure

This study shows that overglycosylation of collagen type I affects fibril structure by altering local packing and sub-band displacements without changing overall periodicity.

Deciphering the intra-tissue specific Collagen PTMs site-specific heterogeneity in Human Adrenal extracellular-matrix

This study identifies and quantifies site-specific collagen post-translational modifications (PTMs) in human adrenal extracellular matrix, revealing intra-tissue heterogeneity in PTM occupancy and potential functional implications.

Evidence for the major role of PH4EFB in the prolyl 4-hydroxylation of Drosophila collagen IV

This study identifies PH4EFB as the primary prolyl 4-hydroxylase for collagen IV in Drosophila through bioinformatic, transcriptomic, and biochemical analyses.

Collagen fibril formation at the plasma membrane occurs independently from collagen secretion

The paper investigates collagen fibril formation in fibroblasts, showing it occurs at the plasma membrane independently of secretion and involves endocytosis.

State diagrams of type I collagen for the rational design of biomimetic materials

This paper establishes state diagrams for type I collagen to understand fibrillogenesis conditions and optimize biomimetic material production.

Reinforcement of Fibrillar Collagen Hydrogels with Bioorthogonal Covalent Crosslinks

This paper develops a method to reinforce collagen hydrogels with bioorthogonal covalent crosslinks while preserving their fibrillar structure for tissue engineering.

Clinical breakthrough: Biomimetic Scaffolds Based on Soluble Collagen for Regenerating Large Volumes of Highly Functional Tissues

This paper presents a collagen-based biomimetic material that successfully regenerates functional tissues in animal models, including sciatic nerve, tendon, and muscle, with high degradation synchronization and regenerative outcomes.

A facile method for fluorescent visualization of newly synthesized fibrous collagen by capturing the allysine aldehyde groups serving as cross-link precursors

The paper describes a method to fluorescently label newly synthesized collagen fibers using DAF-FM and DAR-4M probes to study collagen dynamics in vertebrate tissues.

Decoding Collagen's Thermally Induced Unfolding and Refolding Pathways

This study uses AFM imaging to analyze the thermal unfolding and refolding pathways of collagen types I, III, and IV, identifying sequence-dependent stability features and interchain disulfide bonds as key factors.

Microfluidic Formation of Ultrathin, Handleable Collagen Sheets Exhibiting Toe-heel Tensile Behavior

This paper develops ultrathin collagen sheets with non-linear tensile behavior, mimicking native tissue properties for biomedical applications.

ColBuilder: Flexible structure generation of crosslinked collagen fibrils

ColBuilder generates atomistic models of crosslinked collagen fibrils and validates them through molecular dynamics simulations.

New imaging tools reveal live cellular collagen secretion, fibril dynamics and network organisation.

This study uses advanced imaging techniques to visualize live collagen secretion, fibril dynamics, and network organization in real time.

Molecular dynamics simulations reveal a mechanism of calcium homeostasis driving homotrimerisation and heterotrimerization of type I collagen

This study uses molecular dynamics simulations to show how calcium ions influence the formation of hetero- and homotrimers of type I collagen, explaining the conditions under which homotrimers form.

A Collagen Triple Helix without the Super Helical Twist

The paper designs collagenous assemblies using a peptide self-assembly system and solves their structure with cryo-EM, revealing a non-twisting triple helix conformation that differs from the canonical right-handed triple helix.

Interpenetrating networks of fibrillar and amorphous collagen promote cell spreading and hydrogel stability

This paper introduces an interpenetrating collagen network that supports cell spreading while maintaining hydrogel stability by combining fibrillar and amorphous collagen structures.