Amino Acid Enzymes and Metabolism

Structure-guided development of a potent human B0AT1 inhibitor effective in a mouse model of phenylketonuria

This study develops a potent B0AT1 inhibitor effective in a PKU mouse model by targeting an allosteric site.

Structure and enzymology of glutaminase mutants that disrupt glutamine-glutamate homeostasis and cause neurological disease

This study examines two GLS mutants causing neurological disease, finding they lose glutamate inhibition and phosphate dependency, leading to glutamate accumulation and disrupted homeostasis.

SLC45A4 encodes a peroxisomal putrescine transporter that promotes GABA de novo synthesis

The paper identifies SLC45A4 as a peroxisomal putrescine transporter that enhances GABA de novo synthesis via the AOP pathway.

TXNIP mediates LAT1/SLC7A5 endocytosis to reduce amino acid uptake in cells entering quiescence

The paper identifies TXNIP as a regulator of amino acid uptake during cellular quiescence by mediating endocytosis of the LAT1/SLC7A5 transporter.

Mutational insights into human kynurenine aminotransferase 1: modulation of transamination and β-elimination activities across diverse substrates

This study investigates how mutations in hKYAT1 affect its transamination and β-elimination activities across various substrates, identifying key residues that influence enzyme function.

Saturation kinetics and specificity of transporters for L-arginine and asymmetric dimethylarginine (ADMA) at the blood-brain and blood-CSF barriers.

This study identifies the transporters and kinetic parameters for L-arginine and ADMA across blood-brain and blood-CSF barriers in mice, revealing complex transport mechanisms and potential implications for NO regulation.

Structural basis of the excitatory amino acid transporter 3 substrate recognition

This study determines the cryo-EM structures of EAAT3 bound to different substrates, revealing how conformational changes and sodium binding influence substrate recognition and transport.

Activity regulation of a glutamine amidotransferase bienzyme complex by substrate-induced subunit interface expansion

The paper investigates how substrate-induced subunit interface expansion regulates catalysis in a glutamine amidotransferase complex, revealing a mechanism for synchronized activity across the enzyme family.

SLC7A5 is required for citrulline-dependent growth in arginine limited conditions

This study identifies SLC7A5 as essential for citrulline-dependent growth in arginine-limited conditions and shows its role in citrulline uptake and tumor growth.

Large neutral amino acid levels tune perinatal neuronal excitability and survival

The paper investigates how changes in large neutral amino acid metabolism during development affect neuronal excitability and survival, finding that Slc7a5 deletion disrupts lipid metabolism and leads to long-term circuit dysfunction.

Structural insights into glycine reuptake inhibition

The paper determines the structure of GlyT1 bound to an inhibitor, revealing its mechanism of non-competitive inhibition and informing drug design.

SorCS2 controls functional expression of amino acid transporter EAAT3 to protect neurons from oxidative stress and epilepsy-induced pathology

This study identifies SorCS2 as a regulator of EAAT3 trafficking that protects neurons from oxidative stress and epilepsy-induced damage.