T. P. Stinear

Mycobacterium ulcerans challenge strain selection for a Buruli ulcer controlled human infection model

This paper identifies M. ulcerans JKD8049 as a suitable human challenge strain for Buruli ulcer research due to its stability, antibiotic susceptibility, and ability to produce mycolactone.

A marine sponge-associated mycobacterium closely related to Mycobacterium tuberculosis

This paper describes the discovery of a marine sponge-associated mycobacterium closely related to M. tuberculosis, which shares virulence factors but lacks virulence in mice.

Season of transmission of Ross River/Barmah Forest Virus and Mycobacterium ulcerans closely align in southeastern Australia, supporting mosquitoes as the vector of Buruli ulcer

Tolerance to Haemophilus influenzae infection in human epithelial cells: insights from a primary cell-based model

This study investigates how human nasal epithelial cells respond to H. influenzae infection, revealing an initial inflammatory response followed by tolerance and reactivation of pro-inflammatory cytokines.

The 3'UTR of vigR is required for virulence in Staphylococcus aureus and has expanded through STAR sequence repeat insertions

This study identifies the 3'UTR of vigR as critical for virulence in S. aureus, showing it regulates cell wall metabolism and interacts with mRNAs involved in virulence and antibiotic tolerance.

A transmission chain linking Mycobacterium ulcerans with Aedes notoscriptus mosquitoes, possums and human Buruli ulcer cases in southeastern Australia

The paper investigates the transmission chain of Mycobacterium ulcerans between mosquitoes, possums, and humans in southeastern Australia, finding a significant association between M. ulcerans and Aedes notoscriptus mosquitoes.

Structured surveys of Australian native possum excreta predict Buruli ulcer occurrence in humans

This study uses structured surveys of possum excreta to predict human Buruli ulcer cases, showing improved predictive performance compared to null models.

Novel determinant of antibiotic resistance: a clinically selected Staphylococcus aureus clpP mutant survives daptomycin treatment by reducing binding of the antibiotic and adapting a rod-shaped morphology

The paper identifies a clpP SNP mutation in MRSA that reduces daptomycin binding and enables survival through rod-shaped morphology, contributing to antibiotic resistance.

Uncovering strain- and age-dependent differences in innate immune response to SARS-CoV-2 infection in nasal epithelia using 10X single-cell sequencing

The paper uses single-cell sequencing to compare innate immune responses to SARS-CoV-2 in nasal epithelial cells from adults and adolescents, finding age- and strain-dependent differences.

The two-component regulator WalKR provides an essential link between cell wall homeostasis with DNA replication in Staphylococcus aureus

This study identifies the WalKR two-component system in Staphylococcus aureus as a polyfunctional regulator linking cell wall homeostasis with DNA replication and other essential processes.

A high-throughput cytotoxicity screening platform reveals agr-independent mutations in bacteraemia-associated Staphylococcus aureus that promote intracellular persistence

The paper develops a high-throughput platform to identify S. aureus mutations that enhance intracellular persistence and reduce cytotoxicity, revealing agr-independent pathoadaptive mutations.

Bridging the gap between bioinformatics and the clinical and public health microbiology laboratory: An ISO-accredited genomics workflow for antimicrobial resistance

This paper develops and validates abritAMR, an ISO-accredited bioinformatics platform for detecting antimicrobial resistance genes with high accuracy and specificity.

Global analysis of biosynthetic gene clusters reveals conserved and unique natural products in entomopathogenic nematode-symbiotic bacteria

The paper analyzes biosynthetic gene clusters in entomopathogenic nematode-symbiotic bacteria to identify conserved and unique natural products and their functional roles.

Long-read RNA sequencing identifies polyadenylation elongation and differential transcript usage of host transcripts during SARS-CoV-2 in vitro infection

The paper uses long-read RNA sequencing to study polyadenylation and transcript usage changes in host cells during SARS-CoV-2 infection, identifying differential polyadenylation and transcript usage in infected cells.

Significant Variability exists in the Toxicity of Global Methicillin-resistant Staphylococcus aureus Lineages.

The paper assesses toxicity variability among global MRSA lineages and identifies Agr mutations linked to reduced toxicity, highlighting the role of undefined genes in toxin regulation.

Transcriptional and epi-transcriptional dynamics of SARS-CoV-2 during cellular infection

This study examines SARS-CoV-2 transcriptional and epi-transcriptional dynamics using long-read sequencing in human and primate infection models, revealing distinct patterns of sgRNA regulation and modification.

Validation of a Single-step, Single-tube Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of SARS-CoV-2 RNA

The paper validates a single-step, single-tube RT-LAMP assay for rapid SARS-CoV-2 detection with high specificity and sensitivity.

Small RNA directs symbiosis, virulence, and natural products biosynthesis in entomopathogenic bacteria

This study identifies ArcZ, an Hfq-dependent sRNA, as essential for small molecule production in entomopathogenic bacteria, revealing its broad regulatory role beyond secondary metabolites.

Microbe-metabolite associations linked to the rebounding murine gut microbiome post-colonization with vancomycin resistant Enterococcus faecium

This study investigates how the gut microbiome changes in mice after colonization with vancomycin-resistant Enterococcus faecium, identifying metabolite associations linked to bacterial shifts.

Secreted TAL effectors protect symbiotic bacteria from entrapment within fungal hyphae

The paper shows that BAT proteins from Burkholderia rhizoxinica prevent entrapment of endosymbionts in fungal hyphae, ensuring their survival and symbiosis.

Global analysis of adenylate-forming enzymes reveals β-lactone biosynthesis pathway in pathogenic Nocardia

The paper uses machine learning to predict adenylate-forming enzyme function and substrate specificity, revealing a β-lactone biosynthesis pathway in Nocardia.

High antibody titres induced by protein subunit vaccines against Buruli ulcer using Mycobacterium ulcerans antigens Hsp18 and MUL_3720

This study evaluates the vaccine potential of M. ulcerans antigens Hsp18 and MUL_3720 in mice, finding high antibody titres but no significant protection against Buruli ulcer.

Vaccine-specific immune responses against Mycobacterium ulcerans infection in a low-dose murine challenge model

This study evaluates a prime-boost vaccine against Mycobacterium ulcerans in mice, finding that both the experimental ER vaccine and BCG provide significant protection through distinct immune profiles.

Functional genomics reveals extensive diversity in Staphylococcus epidermidis restriction modification systems compared to Staphylococcus aureus.

The paper identifies extensive diversity in type I restriction modification systems in Staphylococcus epidermidis compared to Staphylococcus aureus, enabling efficient genetic manipulation of clinically relevant strains.

Remodelling of pSK1 Family Plasmids and Enhanced Chlorhexidine Tolerance in Methicillin-Resistant Staphylococcus aureus

This study examines the evolution of pSK1 family plasmids in MRSA ST239 isolates over 32 years, showing their role in enhancing chlorhexidine tolerance and gentamicin resistance.

Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus

The paper identifies zinc binding to the WalK histidine kinase's PAS domain as a negative regulator of the WalKR two-component system in Staphylococcus aureus.

Increasing tolerance of hospital Enterococcus faecium to hand-wash alcohols

The paper shows hospital Enterococcus faecium isolates from 2010 onward are 10-fold more tolerant to alcohol-based disinfectants than older isolates, leading to increased gastrointestinal colonization in mice.