H. J. Kim

Enterohemorrhagic Escherichia coli (EHEC) Disrupts Intestinal Barrier Integrity in Translational Canine Stem Cell-Derived Monolayers

This study develops a canine colonoid-derived monolayer model to study EHEC infection, showing decreased TEER and ZO-1 expression and increased TNF-α production.

Cellular characterization of the mouse collecting lymphatic vessels reveals that lymphatic muscle cells are the innate pacemaker cells

This study identifies lymphatic muscle cells as the pacemaker cells in mouse collecting lymphatic vessels through combined immunofluorescence, scRNA-seq, and electrophysiological analyses.

Interaction between host G3BP and viral nucleocapsid protein regulates SARS-CoV-2 replication

This study identifies the role of G3BP1-N interaction in SARS-CoV-2 replication by showing that disrupting this interaction reduces viral replication and pathogenesis.

Identification of small molecule inhibitors of G3BP-driven stress granule formation

This study identifies two small molecules that inhibit G3BP-driven stress granule formation and dissolve pre-existing granules, showing broad efficacy across cell types and stressors.

A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 is linked to neurodevelopmental disorders

This study identifies a new Karyopherin-β2 binding epitope in HNRNPH2 linked to neurodevelopmental disorders, explaining cytoplasmic accumulation due to nuclear import defects.

Expanding the epitranscriptomic RNA sequencing and modification mapping mass spectrometry toolbox with field asymmetric waveform ion mobility and electrochemical elution liquid chromatography

This paper introduces a combined FAIMS and EMLC approach to improve RNA modification detection and sequencing in complex samples.

A novel meiotic protein required for homolog pairing and regulation of synapsis in C. elegans

This study identifies MJL-1 as a meiosis-specific protein essential for homolog pairing and synapsis in C. elegans by connecting Pairing Centers to LINC complexes.

Harnessing the Intrinsic Photochemistry of Isoxazoles for the Development of Chemoproteomic Crosslinking Methods

This paper introduces a new photo-crosslinking method using isoxazole's intrinsic photochemistry, validated across multiple proteins in vitro and ex vivo.

A conserved long intergenic non-coding RNA containing snoRNA sequences, lncCOBRA1, affects Arabidopsis germination and development

This study identifies lncCOBRA1, a conserved lncRNA in Arabidopsis containing snoRNA domains, showing its role in germination and plant development.

Single-cell transcriptomics elucidates in vitro reprogramming of human intestinal epithelium cultured in a physiodynamic gut-on-a-chip

This study uses single-cell transcriptomics to show that a gut-on-a-chip culture reprograms human intestinal epithelial cells to exhibit in vivo-like heterogeneity and physiological functions.

An efficient and robust HPLC method to determine the sialylation levels of human epithelial cells

This paper develops an HPLC-UV method to quantify sialic acids in cancer cells, showing it is efficient and effective for assessing sialyltransferase inhibitor efficacy.

Structural visualization of de novo initiation of RNA polymerase II transcription

This study reports structures of RNA polymerase II initiation complexes showing TFIIH's role in promoter melting and transcription initiation.

Ubiquitination of G3BP1 mediates stress granule disassembly in a stress-specific manner

The paper identifies ubiquitination of G3BP1 as a mechanism required for heat shock-induced stress granule disassembly through interaction with FAF2 and p97/VCP.

Ubiquitination is essential for recovery of cellular activities followingheat shock

The paper shows that stress-induced ubiquitination is essential for recovery of cellular activities following heat stress.

TMPRSS2 and RNA-dependent RNA polymerase are effective targets of therapeutic intervention for treatment of COVID-19 caused by SARS-CoV-2 variants (B.1.1.7 and B.1.351)

The paper evaluates the effectiveness of TMPRSS2 and RdRp inhibitors against SARS-CoV-2 variants B.1.1.7 and B.1.351, finding they are equally effective as against the original isolate.

A polyyne toxin produced by an antagonistic bacterium blinds and lyses a green microalga

This study identifies a polyyne toxin produced by Pseudomonas protegens that blinds and lyses Chlamydomonas reinhardtii by targeting its eyespot, alongside orfamide A, to disrupt phototaxis.

Exploration of polyyne biosynthetic gene cluster diversity in bacteria leads to the discovery of the Pseudomonas polyyne protegencin

The paper explores polyyne biosynthetic gene clusters in bacteria, identifies a conserved seven-gene cassette in Pseudomonas, and discovers the metabolite protegencin.

Structure of TFIIH/Rad4-Rad23-Rad33 in damaged DNA opening in Nucleotide Excision Repair

The paper presents a cryo-EM structure of an NER initiation complex involving Rad4-Rad23-Rad33 and TFIIH bound to a DNA lesion, revealing DNA unwinding and translocation mechanisms.

Strain variation in Bacillus cereus biofilms and their susceptibility to extracellular matrix-degrading enzymes

This study examines strain variation in Bacillus cereus biofilms and their susceptibility to extracellular matrix-degrading enzymes, revealing distinct structural and compositional differences among strains.

Human spinal cord organoids exhibiting neural tube morphogenesis for a quantifiable drug screening system of neural tube defects

This study develops human spinal cord-like organoids (hSCOs) to enable quantitative drug screening for neural tube defects.

Development of a species diagnostic molecular tool for an invasive pest, Mythimna loreyi using LAMP

This paper develops a LAMP assay for rapid identification of the invasive pest Mythimna loreyi using a species-specific sequence from the NADH dehydrogenase subunit 5 gene.

High fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate

This paper introduces a system for high fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate, bridging in vitro and live cell studies of membraneless organelles.

Nasal commensal, Staphylococcus epidermidis shapes the mucosal environment to prevent influenza virus invasion through Serpine1 induction

This paper shows that Staphylococcus epidermidis in the nasal mucosa induces Serpine1, which blocks influenza virus invasion by inhibiting protease activity and IFN-λ signaling.

COVID-19 patients upregulate toll-like receptor 4-mediated inflammatory signaling that mimics bacterial sepsis

The paper identifies upregulated TLR4-mediated inflammatory signaling in COVID-19 patients, linking it to pathological inflammation and suggesting TLR4 as a therapeutic target.

Cryo-EM Structures of Respiratory bc1-cbb3 type CIII2CIV Super-complex and Electronic Communication Between the Complexes

This paper reports the first cryo-EM structure of a bacterial respiratory bc1-cbb3 super-complex and native CIII2 conformers, revealing structural and electronic communication features between complexes.

E prostanoid receptor 4 expressing macrophages promote the regeneration of the intestinal epithelial barrier upon inflammation

The paper identifies EP4-expressing intestinal macrophages that promote mucosal healing by secreting CXCL1 during inflammation resolution.

Targeted Eicosanoid Profiling Reveals MicroRNA-155 Shifts PGE2/PGD2 Balance Towards Oncogenic State

This study shows miR-155 shifts PGE2/PGD2 balance towards an oncogenic state by regulating enzymes involved in prostaglandin synthesis.

Development of a simple and accurate molecular tool for Spodoptera frugiperda species identification using LAMP

This paper develops a LAMP assay for rapid and accurate identification of Spodoptera frugiperda using a specific mtDNA region, showing high sensitivity and simplicity.

Naringenin as an antibacterial reagent controlling of biofilm formation and fatty acid metabolism in MRSA

This study identifies naringenin as an antibacterial agent that reduces biofilm formation and fatty acid secretion in MRSA and shows synergy with oxacillin.

ThiL is a valid antibacterial target that is essential for both thiamine biosynthesis and salvage pathway in Pseudomonas aeruginosa

This study demonstrates that ThiL is essential for thiamine biosynthesis and salvage in Pseudomonas aeruginosa and identifies inhibitors with potential antibacterial activity.