Gabriele Lignani

Fast-ripples are emergent properties of neuronal networks

Most fast-ripples (250-500 Hz) in rat and human recordings are consistent with chance clustering of action potentials; in awake patients, distinct events make up an estimated 37.5% of detections.

C1q and immunoglobulins mediate activity-dependent synapse loss in the adult brain

This study shows neuronal hyperactivity triggers C1q deposition on synapses, leading to synapse loss, and identifies immunoglobulins as involved in this process.

AAV9-mediated targeting of natural antisense transcript as a novel treatment for Dravet Syndrome

This paper demonstrates the use of AAV9 vectors to deliver AntagoNATs targeting a natural antisense transcript in a DS mouse model, improving survival and reducing seizures.

CANVAS causing AAGGG repeat expansions cause tissue-specific reduction in RFC1 expression and increase sensitivity to DNA damage

AAV delivery of RNA editing machinery rescues SUDEP and seizure phenotype in a mouse model of Dravet Syndrome

This study demonstrates that AAV-delivered RNA editing machinery improves survival and reduces seizures in a mouse model of Dravet Syndrome.

An adaptable, reusable, and light implant for chronic Neuropixels probes

The paper introduces an adaptable implant for chronic Neuropixels recordings in mice, enabling stable, reusable, and lightweight neural recordings across multiple experimental conditions.

Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo

This study demonstrates that CRISPR-CasRx reduces C9orf72 repeat RNAs and DPRs in vitro and in vivo, suggesting its therapeutic potential for ALS/FTD.

Epilepsy-linked kinase CDKL5 phosphorylates voltage-gated calcium channel Cav2.3, altering inactivation kinetics and neuronal excitability

This study identifies CDKL5 as a kinase that phosphorylates Cav2.3, altering its function and contributing to neuronal excitability in CDKL5 deficiency disorder.

Anti-seizure Gene Therapy for Focal Cortical Dysplasia

This paper evaluates gene therapy using Kv1.1 potassium channel overexpression in a mouse model of FCD, showing reduced seizures but no improvement in cognitive or behavioral issues.

Developmental instability of CA1 pyramidal cells in Dravet Syndrome

This study investigates how loss of Scn1a affects CA1 pyramidal cell development and homeostatic plasticity in a Dravet Syndrome model before seizures occur.

REST/NRSF drives homeostatic plasticity of inhibitory synapses in a target-dependent fashion

This study shows REST/NRSF enhances GABAergic inhibition specifically at inhibitory-onto-excitatory synapses in response to hyperactivity, maintaining neuronal homeostasis.

LGI1 downregulation increases neuronal circuit excitability

This study shows that reducing LGI1 levels increases neuronal excitability and synaptic plasticity, potentially contributing to epilepsy and limbic encephalitis.

Alternative splicing tunes sodium channels to support channel- and neuron- specific effects

The paper shows that alternative splicing of sodium channels Nav1.1 and Nav1.2 has cell-type-specific effects on neuronal activity and firing properties.

In vivo CRISPRa decreases seizures and rescues cognitive deficits in a rodent model of epilepsy

This study uses CRISPRa to increase Kv1.1 expression in mouse hippocampal neurons, reducing seizures and cognitive deficits in a temporal lobe epilepsy model.

Olanzapine: a full and potent agonist at the hM4D(Gi) DREADD amenable to clinical translation of chemogenetics

The paper identifies olanzapine as a potent and full agonist for the hM4D(Gi) DREADD, enabling clinical translation of chemogenetics for CNS diseases.